EFFECT OF SPERM CAPACITATION MEDIA ON IN VITRO FERTILIZATION OF ARABI EWES OOCYTES
The study was conducted at Assisted Reproductive Technology Center (A R T) College of Medicine, University of Basra , from January , 2009 to January ,2010 . The aim was to study the effect of capacitation media on in vitro fertilization of Arabi ewes oocytes . A total of 2279 oocytes were collec...
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| Format: | Article |
| Language: | English |
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College of Agriculture
2012-03-01
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| Series: | Mesopotamia Journal of Agriculture |
| Online Access: | https://magrj.mosuljournals.com/article_63474_1709f39537d85f89a10dc34e1e8d261d.pdf |
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| author | Taha .J . Al-Taha |
| author_facet | Taha .J . Al-Taha |
| author_sort | Taha .J . Al-Taha |
| collection | DOAJ |
| description | The study was conducted at Assisted Reproductive Technology Center (A R T) College of Medicine, University of Basra , from January , 2009 to January ,2010 . The aim was to study the effect of capacitation media on in vitro fertilization of Arabi ewes oocytes . A total of 2279 oocytes were collected from ovaries obtained from Basra Slaughter House .Oocytes were matured in TCM199 (tissue culture medium 199) supplemented with10%Fetal bovin serum (FBS) to study the effect of capacitation media on their fertilizing ability. freshly ejaculated and frozen thawed spermatozoa were capacitated in 1 of 2 media .A Bracket culture medium or Earle’s balanced salt medium . Results revealed that Bracket medium with Hyalouronidase enzyme and Gentamycin instead of Penicillin antibiotics showed significant increase (P≤ 0.05) in number of in vitro fertilized and divided ewe’s oocytes as compared with the Earle’s in both fresh and frozen thawed spermatozoa . |
| format | Article |
| id | doaj-art-0d7dc2b5ba5246c191b95b4e00c28781 |
| institution | OA Journals |
| issn | 1815-316X 2224-9796 |
| language | English |
| publishDate | 2012-03-01 |
| publisher | College of Agriculture |
| record_format | Article |
| series | Mesopotamia Journal of Agriculture |
| spelling | doaj-art-0d7dc2b5ba5246c191b95b4e00c287812025-08-20T02:03:00ZengCollege of AgricultureMesopotamia Journal of Agriculture1815-316X2224-97962012-03-01400525710.33899/magrj.2012.6347463474EFFECT OF SPERM CAPACITATION MEDIA ON IN VITRO FERTILIZATION OF ARABI EWES OOCYTESTaha .J . Al-TahaThe study was conducted at Assisted Reproductive Technology Center (A R T) College of Medicine, University of Basra , from January , 2009 to January ,2010 . The aim was to study the effect of capacitation media on in vitro fertilization of Arabi ewes oocytes . A total of 2279 oocytes were collected from ovaries obtained from Basra Slaughter House .Oocytes were matured in TCM199 (tissue culture medium 199) supplemented with10%Fetal bovin serum (FBS) to study the effect of capacitation media on their fertilizing ability. freshly ejaculated and frozen thawed spermatozoa were capacitated in 1 of 2 media .A Bracket culture medium or Earle’s balanced salt medium . Results revealed that Bracket medium with Hyalouronidase enzyme and Gentamycin instead of Penicillin antibiotics showed significant increase (P≤ 0.05) in number of in vitro fertilized and divided ewe’s oocytes as compared with the Earle’s in both fresh and frozen thawed spermatozoa .https://magrj.mosuljournals.com/article_63474_1709f39537d85f89a10dc34e1e8d261d.pdf |
| spellingShingle | Taha .J . Al-Taha EFFECT OF SPERM CAPACITATION MEDIA ON IN VITRO FERTILIZATION OF ARABI EWES OOCYTES Mesopotamia Journal of Agriculture |
| title | EFFECT OF SPERM CAPACITATION MEDIA ON IN VITRO FERTILIZATION OF ARABI EWES OOCYTES |
| title_full | EFFECT OF SPERM CAPACITATION MEDIA ON IN VITRO FERTILIZATION OF ARABI EWES OOCYTES |
| title_fullStr | EFFECT OF SPERM CAPACITATION MEDIA ON IN VITRO FERTILIZATION OF ARABI EWES OOCYTES |
| title_full_unstemmed | EFFECT OF SPERM CAPACITATION MEDIA ON IN VITRO FERTILIZATION OF ARABI EWES OOCYTES |
| title_short | EFFECT OF SPERM CAPACITATION MEDIA ON IN VITRO FERTILIZATION OF ARABI EWES OOCYTES |
| title_sort | effect of sperm capacitation media on in vitro fertilization of arabi ewes oocytes |
| url | https://magrj.mosuljournals.com/article_63474_1709f39537d85f89a10dc34e1e8d261d.pdf |
| work_keys_str_mv | AT tahajaltaha effectofspermcapacitationmediaoninvitrofertilizationofarabiewesoocytes |