Characterizing the extractable proteins from tomato leaves – A proteomics study
The ambition to utilize agricultural by-products has spotlighted tomato leaves as a promising source for plant-based proteins. High-yielding protein extractability is key for its industrial use, but previous studies reported decreased protein extractability at later stages of plant development. This...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157524010022 |
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| author | Marietheres Kleuter Yafei Yu Lukas Verdegaal Francesco Pancaldi Antoine H.P. America Atze Jan van der Goot Luisa M. Trindade |
| author_facet | Marietheres Kleuter Yafei Yu Lukas Verdegaal Francesco Pancaldi Antoine H.P. America Atze Jan van der Goot Luisa M. Trindade |
| author_sort | Marietheres Kleuter |
| collection | DOAJ |
| description | The ambition to utilize agricultural by-products has spotlighted tomato leaves as a promising source for plant-based proteins. High-yielding protein extractability is key for its industrial use, but previous studies reported decreased protein extractability at later stages of plant development. This study investigated the underlying factors in protein extractability through a comprehensive proteomics analysis across four plant developmental stages (vegetative, flowering, fruit-forming, mature-fruit). The findings linked reduced yields to a shift in leaf function, from anabolic to catabolic processes and (a)biotic stress responses. This functional shift is accompanied by decreased protein synthesis and increased protein degradation, leading to an overall decrease of the soluble protein fraction. Furthermore, incomplete extraction of soluble proteins from leaves of later developmental stages, suggested the presence of inhibitory molecules hindering the extraction process. These findings indicate that breeding strategies towards increased amounts of soluble proteins and reduced concentration of inhibitory molecules could enhance protein extraction yields. |
| format | Article |
| id | doaj-art-0d7ae415ec6f4b31ad450d3d3ca4d1f2 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-0d7ae415ec6f4b31ad450d3d3ca4d1f22025-02-12T05:32:01ZengElsevierFood Chemistry: X2590-15752025-01-0125102114Characterizing the extractable proteins from tomato leaves – A proteomics studyMarietheres Kleuter0Yafei Yu1Lukas Verdegaal2Francesco Pancaldi3Antoine H.P. America4Atze Jan van der Goot5Luisa M. Trindade6Plant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, the NetherlandsLaboratory of Food Process Engineering, Wageningen University, PO Box 17, 6700, AA, Wageningen, the NetherlandsPlant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, the NetherlandsPlant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, the NetherlandsBU Bioscience, Wageningen Research, Droevendaalsesteeg 1, 6708, PB, Wageningen, the NetherlandsLaboratory of Food Process Engineering, Wageningen University, PO Box 17, 6700, AA, Wageningen, the NetherlandsPlant Breeding, Wageningen University, Droevendaalsesteeg 1, 6708, PB, Wageningen, the Netherlands; Corresponding author at: PO Box 386, 6700, AJ, Wageningen, the Netherlands.The ambition to utilize agricultural by-products has spotlighted tomato leaves as a promising source for plant-based proteins. High-yielding protein extractability is key for its industrial use, but previous studies reported decreased protein extractability at later stages of plant development. This study investigated the underlying factors in protein extractability through a comprehensive proteomics analysis across four plant developmental stages (vegetative, flowering, fruit-forming, mature-fruit). The findings linked reduced yields to a shift in leaf function, from anabolic to catabolic processes and (a)biotic stress responses. This functional shift is accompanied by decreased protein synthesis and increased protein degradation, leading to an overall decrease of the soluble protein fraction. Furthermore, incomplete extraction of soluble proteins from leaves of later developmental stages, suggested the presence of inhibitory molecules hindering the extraction process. These findings indicate that breeding strategies towards increased amounts of soluble proteins and reduced concentration of inhibitory molecules could enhance protein extraction yields.http://www.sciencedirect.com/science/article/pii/S2590157524010022Protein extractionTomato – Solanum lycopersicumPlant developmentAgricultural by-productsProteomics analysisSoluble proteins |
| spellingShingle | Marietheres Kleuter Yafei Yu Lukas Verdegaal Francesco Pancaldi Antoine H.P. America Atze Jan van der Goot Luisa M. Trindade Characterizing the extractable proteins from tomato leaves – A proteomics study Food Chemistry: X Protein extraction Tomato – Solanum lycopersicum Plant development Agricultural by-products Proteomics analysis Soluble proteins |
| title | Characterizing the extractable proteins from tomato leaves – A proteomics study |
| title_full | Characterizing the extractable proteins from tomato leaves – A proteomics study |
| title_fullStr | Characterizing the extractable proteins from tomato leaves – A proteomics study |
| title_full_unstemmed | Characterizing the extractable proteins from tomato leaves – A proteomics study |
| title_short | Characterizing the extractable proteins from tomato leaves – A proteomics study |
| title_sort | characterizing the extractable proteins from tomato leaves a proteomics study |
| topic | Protein extraction Tomato – Solanum lycopersicum Plant development Agricultural by-products Proteomics analysis Soluble proteins |
| url | http://www.sciencedirect.com/science/article/pii/S2590157524010022 |
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