Irisin Enhances Osteoblast Differentiation In Vitro
It has been recently demonstrated that exercise activity increases the expression of the myokine Irisin in skeletal muscle, which is able to drive the transition of white to brown adipocytes, likely following a phenomenon of transdifferentiation. This new evidence supports the idea that muscle can...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2014-01-01
|
| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2014/902186 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849308703921012736 |
|---|---|
| author | Graziana Colaianni Concetta Cuscito Teresa Mongelli Angela Oranger Giorgio Mori Giacomina Brunetti Silvia Colucci Saverio Cinti Maria Grano |
| author_facet | Graziana Colaianni Concetta Cuscito Teresa Mongelli Angela Oranger Giorgio Mori Giacomina Brunetti Silvia Colucci Saverio Cinti Maria Grano |
| author_sort | Graziana Colaianni |
| collection | DOAJ |
| description | It has been recently demonstrated that exercise activity increases the expression of the myokine Irisin in skeletal muscle, which is able to drive the transition of white to brown adipocytes, likely following a phenomenon of transdifferentiation. This new evidence supports the idea that muscle can be considered an endocrine organ, given its ability to target adipose tissue by promoting energy expenditure. In accordance with these new findings, we hypothesized that Irisin is directly involved in bone metabolism, demonstrating its ability to increase the differentiation of bone marrow stromal cells into mature osteoblasts.
Firstly, we confirmed that myoblasts from mice subjected to 3 weeks of free wheel running increased Irisin expression compared to nonexercised state. The conditioned media (CM) collected from myoblasts of exercised mice induced osteoblast differentiation in vitro to a greater extent than those of mice housed in resting conditions. Furthermore, the differentiated osteoblasts increased alkaline phosphatase and collagen I expression by an Irisin-dependent mechanism. Our results show, for the first time, that Irisin directly targets osteoblasts, enhancing their differentiation. This finding advances notable perspectives in future studies which could satisfy the ongoing research of exercise-mimetic therapies with anabolic action on the skeleton. |
| format | Article |
| id | doaj-art-0d74bbd8efb643059fb853193cffc774 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-0d74bbd8efb643059fb853193cffc7742025-08-20T03:54:24ZengWileyInternational Journal of Endocrinology1687-83371687-83452014-01-01201410.1155/2014/902186902186Irisin Enhances Osteoblast Differentiation In VitroGraziana Colaianni0Concetta Cuscito1Teresa Mongelli2Angela Oranger3Giorgio Mori4Giacomina Brunetti5Silvia Colucci6Saverio Cinti7Maria Grano8Department of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124 Bari, ItalyDepartment of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124 Bari, ItalyDepartment of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124 Bari, ItalyDepartment of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124 Bari, ItalyDepartment of Clinical and Experimental Medicine, University of Foggia, 71100 Foggia, ItalyDepartment of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124 Bari, ItalyDepartment of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124 Bari, ItalyDepartment of Experimental and Clinical Medicine, Center of Obesity, United Hospitals—University of Ancona, 60020 Ancona, ItalyDepartment of Basic Medical Science, Neuroscience and Sense Organs, University of Bari, 70124 Bari, ItalyIt has been recently demonstrated that exercise activity increases the expression of the myokine Irisin in skeletal muscle, which is able to drive the transition of white to brown adipocytes, likely following a phenomenon of transdifferentiation. This new evidence supports the idea that muscle can be considered an endocrine organ, given its ability to target adipose tissue by promoting energy expenditure. In accordance with these new findings, we hypothesized that Irisin is directly involved in bone metabolism, demonstrating its ability to increase the differentiation of bone marrow stromal cells into mature osteoblasts. Firstly, we confirmed that myoblasts from mice subjected to 3 weeks of free wheel running increased Irisin expression compared to nonexercised state. The conditioned media (CM) collected from myoblasts of exercised mice induced osteoblast differentiation in vitro to a greater extent than those of mice housed in resting conditions. Furthermore, the differentiated osteoblasts increased alkaline phosphatase and collagen I expression by an Irisin-dependent mechanism. Our results show, for the first time, that Irisin directly targets osteoblasts, enhancing their differentiation. This finding advances notable perspectives in future studies which could satisfy the ongoing research of exercise-mimetic therapies with anabolic action on the skeleton.http://dx.doi.org/10.1155/2014/902186 |
| spellingShingle | Graziana Colaianni Concetta Cuscito Teresa Mongelli Angela Oranger Giorgio Mori Giacomina Brunetti Silvia Colucci Saverio Cinti Maria Grano Irisin Enhances Osteoblast Differentiation In Vitro International Journal of Endocrinology |
| title | Irisin Enhances Osteoblast Differentiation In Vitro |
| title_full | Irisin Enhances Osteoblast Differentiation In Vitro |
| title_fullStr | Irisin Enhances Osteoblast Differentiation In Vitro |
| title_full_unstemmed | Irisin Enhances Osteoblast Differentiation In Vitro |
| title_short | Irisin Enhances Osteoblast Differentiation In Vitro |
| title_sort | irisin enhances osteoblast differentiation in vitro |
| url | http://dx.doi.org/10.1155/2014/902186 |
| work_keys_str_mv | AT grazianacolaianni irisinenhancesosteoblastdifferentiationinvitro AT concettacuscito irisinenhancesosteoblastdifferentiationinvitro AT teresamongelli irisinenhancesosteoblastdifferentiationinvitro AT angelaoranger irisinenhancesosteoblastdifferentiationinvitro AT giorgiomori irisinenhancesosteoblastdifferentiationinvitro AT giacominabrunetti irisinenhancesosteoblastdifferentiationinvitro AT silviacolucci irisinenhancesosteoblastdifferentiationinvitro AT saveriocinti irisinenhancesosteoblastdifferentiationinvitro AT mariagrano irisinenhancesosteoblastdifferentiationinvitro |