Microbiological characteristics of hypermucoviscous Klebsiella pneumoniae isolates from different body fluids

Introduction: Reports of hypermucoviscous Klebsiella pneumoniae (hvKP) isolated from fluids other than blood or abscess are rare. The aim of the study was to compare clinical and microbiological characteristics of hvKP found in blood or abscess fluid with those isolated from other loci. Methodol...

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Main Authors: Xiumei Xiao, Bei Yao, Qingtao Zhou, Jie Zhang
Format: Article
Language:English
Published: The Journal of Infection in Developing Countries 2017-09-01
Series:Journal of Infection in Developing Countries
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Online Access:https://jidc.org/index.php/journal/article/view/8132
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author Xiumei Xiao
Bei Yao
Qingtao Zhou
Jie Zhang
author_facet Xiumei Xiao
Bei Yao
Qingtao Zhou
Jie Zhang
author_sort Xiumei Xiao
collection DOAJ
description Introduction: Reports of hypermucoviscous Klebsiella pneumoniae (hvKP) isolated from fluids other than blood or abscess are rare. The aim of the study was to compare clinical and microbiological characteristics of hvKP found in blood or abscess fluid with those isolated from other loci. Methodology: A total of 24 non-repetitive hvKP isolates were collected from January 2013 to June 2014 from patients with hvKP infections. There were 15 in Group 1 (fluid other than blood or abscess) and 9 in Group 2 (blood or abscess fluid). Medical records of all patients were reviewed. Capsular polysaccharide (CPS) typing, virulence factor determination, and multilocus sequence typing (MLST) of hvKP isolates were performed. Results: Seventeen sequence types (STs) and 6 capsular serotypes were identified. Type K2CC65 was most commonly identified in Group 1 and type K2CC86 in Group 2. Deletion of pLVPK-derived loci were found in K2 and non-K1/K2 hvKP strains. Two virulent genes, fimH and ycfM, were identified more frequently in Group 2 than in Group 1. There was no difference in the frequency of other virulent genes or serotypes in the two groups. Two imipenem resistant hvKP isolates (cr-hvKP) were found in non-blood or abscess samples. Conclusions: hvKP isolated from different body fluids had similar clinical and microbiological characteristics. cr-hvKP identified in non-blood or abscess samples should raise our attention to the challenging situation and management of hvKP infection.
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spelling doaj-art-0d58c9a650e544faa94f987cb4d615dc2025-08-20T02:27:23ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-09-01110910.3855/jidc.8132Microbiological characteristics of hypermucoviscous Klebsiella pneumoniae isolates from different body fluidsXiumei Xiao0Bei Yao1Qingtao Zhou2Jie Zhang3Peking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, ChinaPeking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, ChinaPeking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, ChinaPeking University Third Hospital, No. 49 North Garden Road, Haidian District, Beijing 100191, China Introduction: Reports of hypermucoviscous Klebsiella pneumoniae (hvKP) isolated from fluids other than blood or abscess are rare. The aim of the study was to compare clinical and microbiological characteristics of hvKP found in blood or abscess fluid with those isolated from other loci. Methodology: A total of 24 non-repetitive hvKP isolates were collected from January 2013 to June 2014 from patients with hvKP infections. There were 15 in Group 1 (fluid other than blood or abscess) and 9 in Group 2 (blood or abscess fluid). Medical records of all patients were reviewed. Capsular polysaccharide (CPS) typing, virulence factor determination, and multilocus sequence typing (MLST) of hvKP isolates were performed. Results: Seventeen sequence types (STs) and 6 capsular serotypes were identified. Type K2CC65 was most commonly identified in Group 1 and type K2CC86 in Group 2. Deletion of pLVPK-derived loci were found in K2 and non-K1/K2 hvKP strains. Two virulent genes, fimH and ycfM, were identified more frequently in Group 2 than in Group 1. There was no difference in the frequency of other virulent genes or serotypes in the two groups. Two imipenem resistant hvKP isolates (cr-hvKP) were found in non-blood or abscess samples. Conclusions: hvKP isolated from different body fluids had similar clinical and microbiological characteristics. cr-hvKP identified in non-blood or abscess samples should raise our attention to the challenging situation and management of hvKP infection. https://jidc.org/index.php/journal/article/view/8132Hypermucoviscous Klebsiella pneumoniaehypermucoviscous Klebsiella pneumoniaemicrobiological characteristicsnon-blood/abscess samplevirulencefluid
spellingShingle Xiumei Xiao
Bei Yao
Qingtao Zhou
Jie Zhang
Microbiological characteristics of hypermucoviscous Klebsiella pneumoniae isolates from different body fluids
Journal of Infection in Developing Countries
Hypermucoviscous Klebsiella pneumoniae
hypermucoviscous Klebsiella pneumoniae
microbiological characteristics
non-blood/abscess sample
virulence
fluid
title Microbiological characteristics of hypermucoviscous Klebsiella pneumoniae isolates from different body fluids
title_full Microbiological characteristics of hypermucoviscous Klebsiella pneumoniae isolates from different body fluids
title_fullStr Microbiological characteristics of hypermucoviscous Klebsiella pneumoniae isolates from different body fluids
title_full_unstemmed Microbiological characteristics of hypermucoviscous Klebsiella pneumoniae isolates from different body fluids
title_short Microbiological characteristics of hypermucoviscous Klebsiella pneumoniae isolates from different body fluids
title_sort microbiological characteristics of hypermucoviscous klebsiella pneumoniae isolates from different body fluids
topic Hypermucoviscous Klebsiella pneumoniae
hypermucoviscous Klebsiella pneumoniae
microbiological characteristics
non-blood/abscess sample
virulence
fluid
url https://jidc.org/index.php/journal/article/view/8132
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AT qingtaozhou microbiologicalcharacteristicsofhypermucoviscousklebsiellapneumoniaeisolatesfromdifferentbodyfluids
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