Separation of intact intestinal epithelium from mesenchyme
Current protocols for separating adult intestinal epithelial cells from the underlying muscular and mesenchymal tissues typically involve extended incubations, harsh mechanical treatment, and exposure to either proteases or chelating agents. The drawbacks of these approaches include fragmentation, c...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2013-07-01
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| Series: | BioTechniques |
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| Online Access: | https://www.future-science.com/doi/10.2144/000114055 |
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| _version_ | 1850152192217972736 |
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| author | Ali Moussavi Nik Peter Carlsson |
| author_facet | Ali Moussavi Nik Peter Carlsson |
| author_sort | Ali Moussavi Nik |
| collection | DOAJ |
| description | Current protocols for separating adult intestinal epithelial cells from the underlying muscular and mesenchymal tissues typically involve extended incubations, harsh mechanical treatment, and exposure to either proteases or chelating agents. The drawbacks of these approaches include fragmentation, contamination with other cell types, reduced viability, and under-representation of crypt cells. Here we describe a gentle procedure that allows harvesting of pure, fully viable sheets of murine intestinal epithelium, with intact crypts and villi, without enzymes or EDTA. The mesenchyme retains intact villus core projections, is virtually free from epithelial cells, and can be cultured in vitro. |
| format | Article |
| id | doaj-art-0d582153a6a04561b337cb31cb4a9250 |
| institution | OA Journals |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2013-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-0d582153a6a04561b337cb31cb4a92502025-08-20T02:26:03ZengTaylor & Francis GroupBioTechniques0736-62051940-98182013-07-01551424410.2144/000114055Separation of intact intestinal epithelium from mesenchymeAli Moussavi Nik0Peter Carlsson11Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden1Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, SwedenCurrent protocols for separating adult intestinal epithelial cells from the underlying muscular and mesenchymal tissues typically involve extended incubations, harsh mechanical treatment, and exposure to either proteases or chelating agents. The drawbacks of these approaches include fragmentation, contamination with other cell types, reduced viability, and under-representation of crypt cells. Here we describe a gentle procedure that allows harvesting of pure, fully viable sheets of murine intestinal epithelium, with intact crypts and villi, without enzymes or EDTA. The mesenchyme retains intact villus core projections, is virtually free from epithelial cells, and can be cultured in vitro.https://www.future-science.com/doi/10.2144/000114055intestineepitheliummesenchymeseparation |
| spellingShingle | Ali Moussavi Nik Peter Carlsson Separation of intact intestinal epithelium from mesenchyme BioTechniques intestine epithelium mesenchyme separation |
| title | Separation of intact intestinal epithelium from mesenchyme |
| title_full | Separation of intact intestinal epithelium from mesenchyme |
| title_fullStr | Separation of intact intestinal epithelium from mesenchyme |
| title_full_unstemmed | Separation of intact intestinal epithelium from mesenchyme |
| title_short | Separation of intact intestinal epithelium from mesenchyme |
| title_sort | separation of intact intestinal epithelium from mesenchyme |
| topic | intestine epithelium mesenchyme separation |
| url | https://www.future-science.com/doi/10.2144/000114055 |
| work_keys_str_mv | AT alimoussavinik separationofintactintestinalepitheliumfrommesenchyme AT petercarlsson separationofintactintestinalepitheliumfrommesenchyme |