Atherogenic ω-6 Lipids Modulate PPAR- EGR-1 Crosstalk in Vascular Cells
Atherogenic ω-6 lipids are physiological ligands of peroxisome proliferator-activated receptors (PPARs) and elicit pro- and antiatherogenic responses in vascular cells. The objective of this study was to investigate if ω-6 lipids modulated the early growth response-1 (Egr-1)/PPAR crosstalk thereby a...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2011/753917 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832565142076784640 |
|---|---|
| author | Jia Fei Carla Cook Miriah Gillespie Bangning Yu Khyra Fullen Nalini Santanam |
| author_facet | Jia Fei Carla Cook Miriah Gillespie Bangning Yu Khyra Fullen Nalini Santanam |
| author_sort | Jia Fei |
| collection | DOAJ |
| description | Atherogenic ω-6 lipids are physiological ligands of peroxisome proliferator-activated receptors (PPARs) and elicit pro- and antiatherogenic responses in vascular cells. The objective of this study was to investigate if ω-6 lipids modulated the early growth response-1 (Egr-1)/PPAR crosstalk thereby altering vascular function. Rat aortic smooth muscle cells (RASMCs) were exposed to ω-6 lipids, linoleic acid (LA), or its oxidized form, 13-HPODE (OxLA) in the presence or absence of a PPARα antagonist (MK886) or PPARγ antagonist (GW9662) or PPAR-specific siRNA. Our results demonstrate that ω-6 lipids, induced Egr-1 and monocyte chemotactic protein-1 (MCP-1) mRNA and protein levels at the acute phase (1–4 hrs) when PPARα was downregulated and at subacute phase (4–12 hrs) by modulating PPARγ, thus resulting in altered monocyte adhesion to RASMCs. We provide novel insights into the mechanism of action of ω-6 lipids on Egr-1/PPAR interactions in vascular cells and their potential in altering vascular function. |
| format | Article |
| id | doaj-art-0d545510e3584b9d9dcf5d691f75000e |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-0d545510e3584b9d9dcf5d691f75000e2025-02-03T01:09:00ZengWileyPPAR Research1687-47571687-47652011-01-01201110.1155/2011/753917753917Atherogenic ω-6 Lipids Modulate PPAR- EGR-1 Crosstalk in Vascular CellsJia Fei0Carla Cook1Miriah Gillespie2Bangning Yu3Khyra Fullen4Nalini Santanam5Department of Pharmacology, Physiology and Toxicology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USADepartment of Pharmacology, Physiology and Toxicology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USADepartment of Pharmacology, Physiology and Toxicology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USADepartment of Pharmacology and Experimental Therapeutics, LSU Health Sciences Center, New Orleans, LA, USADepartment of Pharmacology, Physiology and Toxicology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USADepartment of Pharmacology, Physiology and Toxicology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USAAtherogenic ω-6 lipids are physiological ligands of peroxisome proliferator-activated receptors (PPARs) and elicit pro- and antiatherogenic responses in vascular cells. The objective of this study was to investigate if ω-6 lipids modulated the early growth response-1 (Egr-1)/PPAR crosstalk thereby altering vascular function. Rat aortic smooth muscle cells (RASMCs) were exposed to ω-6 lipids, linoleic acid (LA), or its oxidized form, 13-HPODE (OxLA) in the presence or absence of a PPARα antagonist (MK886) or PPARγ antagonist (GW9662) or PPAR-specific siRNA. Our results demonstrate that ω-6 lipids, induced Egr-1 and monocyte chemotactic protein-1 (MCP-1) mRNA and protein levels at the acute phase (1–4 hrs) when PPARα was downregulated and at subacute phase (4–12 hrs) by modulating PPARγ, thus resulting in altered monocyte adhesion to RASMCs. We provide novel insights into the mechanism of action of ω-6 lipids on Egr-1/PPAR interactions in vascular cells and their potential in altering vascular function.http://dx.doi.org/10.1155/2011/753917 |
| spellingShingle | Jia Fei Carla Cook Miriah Gillespie Bangning Yu Khyra Fullen Nalini Santanam Atherogenic ω-6 Lipids Modulate PPAR- EGR-1 Crosstalk in Vascular Cells PPAR Research |
| title | Atherogenic ω-6 Lipids Modulate PPAR- EGR-1 Crosstalk in Vascular Cells |
| title_full | Atherogenic ω-6 Lipids Modulate PPAR- EGR-1 Crosstalk in Vascular Cells |
| title_fullStr | Atherogenic ω-6 Lipids Modulate PPAR- EGR-1 Crosstalk in Vascular Cells |
| title_full_unstemmed | Atherogenic ω-6 Lipids Modulate PPAR- EGR-1 Crosstalk in Vascular Cells |
| title_short | Atherogenic ω-6 Lipids Modulate PPAR- EGR-1 Crosstalk in Vascular Cells |
| title_sort | atherogenic ω 6 lipids modulate ppar egr 1 crosstalk in vascular cells |
| url | http://dx.doi.org/10.1155/2011/753917 |
| work_keys_str_mv | AT jiafei atherogenicō6lipidsmodulatepparegr1crosstalkinvascularcells AT carlacook atherogenicō6lipidsmodulatepparegr1crosstalkinvascularcells AT miriahgillespie atherogenicō6lipidsmodulatepparegr1crosstalkinvascularcells AT bangningyu atherogenicō6lipidsmodulatepparegr1crosstalkinvascularcells AT khyrafullen atherogenicō6lipidsmodulatepparegr1crosstalkinvascularcells AT nalinisantanam atherogenicō6lipidsmodulatepparegr1crosstalkinvascularcells |