THERAPEUTIC ASSESSMENT OF LEMON PEEL EXTRACT MEDIATED GOLD NANOPARTICLES AGAINST LEAD AND ARSENIC INDUCED REPRODUCTIVE TOXICITY IN MALE WISTAR RATS
Gold has been used since ancient times as a rejuvenator, and reproduction enhancer in males and can be used for alleviating reproductive toxicity. Arsenic (As) and lead (Pb) exposure caused multiorgan toxicity, including reproductive organs. Therefore, a study was planned to assess the potential of...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
West Bengal Veterinary Alumni Association
2024-12-01
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| Series: | Exploratory Animal and Medical Research |
| Subjects: | |
| Online Access: | https://animalmedicalresearch.org/Vol.14_Issue-2_December_2024/THERAPEUTIC%20ASSESSMENT%20OF%20LEMON%20PEEL%20EXTRACT%20MEDIATED%20GOLD.pdf |
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| Summary: | Gold has been used since ancient times as a rejuvenator, and reproduction enhancer in males
and can be used for alleviating reproductive toxicity. Arsenic (As) and lead (Pb) exposure caused multiorgan toxicity, including reproductive organs. Therefore, a study was planned to assess the potential of
lemon peel extract-mediated gold nanoparticles (LPGNP) on As and Pb-induced reproductive toxicity in
male rats. The LPGNP synthesized with lemon peel extract and HAuCl4 and characterized by UV-VIS
spectral analysis, FTIR spectrometry, XRD analysis, Zeta potential measurement, NTA and FESEM.
Sodium Arsenate and Lead Acetate were administered to rats @13.8mg/kg and 116.4 mg/kg, respectively for
14 days followed by LPGNP supplementation at @10 and 20 mg/kg for 6 weeks. Alterations in reproductive
parameters, antioxidant enzymes and histopathology of testes were recorded from different groups. In the
results, GNP formation is indicated by changes from pale yellow to dark purple color formation in a
combined solution of lemon peel extract and HAuCl4
. The peak absorbance at 557 nm confirms the synthesis
of GNP. The LPGNP measures from 10 nm to 120 nm and the concentration was found to be 2.5 X 109
particles/ml. Our study showed serum testosterone concentration, sperm motility, total sperm count, sperm
abnormalities, and sperm viability altered significantly in As and Pb toxic groups whereas LPGNP
supplementation caused marked improvement. In histopathology of the testis, As and Pb caused degenerative
changes of seminiferous tubules and sloughing of spermatogenic cells. These changes were observed as
minimal in LPGNP-treated rats. Thus, LPGNP supplementation caused significant improvement against As
and Pb-induced deleterious effects on the reproductive system. |
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| ISSN: | 2277-470X 2319-247X |