Considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivo
Abstract Bone tissue engineering aims to harness materials to develop functional bone tissue to heal ‘critical-sized’ bone defects. This study examined a robust, coated poly(caprolactone) trimethacrylate (PCL-TMA) 3D-printable scaffold designed to augment bone formation. Following optimisation of th...
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Nature Portfolio
2024-10-01
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| Online Access: | https://doi.org/10.1038/s41598-024-75198-3 |
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| author | Karen M. Marshall Jonathan P. Wojciechowski Vineetha Jayawarna Abshar Hasan Cécile Echalier Øystein Øvrebø Tao Yang Kun Zhou Janos M. Kanczler Alvaro Mata Manuel Salmeron-Sanchez Molly M. Stevens Richard O. C. Oreffo |
| author_facet | Karen M. Marshall Jonathan P. Wojciechowski Vineetha Jayawarna Abshar Hasan Cécile Echalier Øystein Øvrebø Tao Yang Kun Zhou Janos M. Kanczler Alvaro Mata Manuel Salmeron-Sanchez Molly M. Stevens Richard O. C. Oreffo |
| author_sort | Karen M. Marshall |
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| description | Abstract Bone tissue engineering aims to harness materials to develop functional bone tissue to heal ‘critical-sized’ bone defects. This study examined a robust, coated poly(caprolactone) trimethacrylate (PCL-TMA) 3D-printable scaffold designed to augment bone formation. Following optimisation of the coatings, three bioactive coatings were examined, i) elastin-like polypeptide (ELP), ii) poly(ethyl acrylate) (PEA), fibronectin (FN) and bone morphogenetic protein-2 (BMP-2) applied sequentially (PEA/FN/BMP-2) and iii) both ELP and PEA/FN/BMP-2 coatings applied concurrently. The scaffold material was robust and showed biodegradability. The coatings demonstrated a significant (p < 0.05) osteogenic response in vitro in alkaline phosphatase gene upregulation and alkaline phosphatase production. The PCL-TMA scaffold and coatings supported angiogenesis and displayed excellent biocompatibility following evaluation on the chorioallantoic membrane assay. No significant (p < 0.05) heterotopic bone formed on the scaffolds within a murine subcutaneous implantation model, compared to the positive control of BMP-2 loaded collagen sponge following examination by micro-computed tomography or histology. The current studies demonstrate a range of innovative coated scaffold constructs with in vitro efficacy and clearly illustrate the importance of an appropriate in vivo environment to validate in vitro functionality prior to scale up and preclinical application. |
| format | Article |
| id | doaj-art-0d419b7d66ce40a3b41f8cab151ffb49 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-0d419b7d66ce40a3b41f8cab151ffb492025-08-20T02:18:28ZengNature PortfolioScientific Reports2045-23222024-10-0114111810.1038/s41598-024-75198-3Considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivoKaren M. Marshall0Jonathan P. Wojciechowski1Vineetha Jayawarna2Abshar Hasan3Cécile Echalier4Øystein Øvrebø5Tao Yang6Kun Zhou7Janos M. Kanczler8Alvaro Mata9Manuel Salmeron-Sanchez10Molly M. Stevens11Richard O. C. Oreffo12Bone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of SouthamptonDepartment of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College LondonSchool of Engineering, Centre for the Cellular Microenvironment, Advanced Research Centre, University of GlasgowSchool of Pharmacy, University of NottinghamDepartment of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College LondonDepartment of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College LondonDepartment of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College LondonDepartment of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College LondonBone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of SouthamptonSchool of Pharmacy, University of NottinghamSchool of Engineering, Centre for the Cellular Microenvironment, Advanced Research Centre, University of GlasgowDepartment of Materials, Department of Bioengineering and Institute for Biomedical Engineering, Imperial College LondonBone and Joint Research Group, Centre for Human Development, Stem Cells and Regeneration, Institute of Developmental Sciences, University of SouthamptonAbstract Bone tissue engineering aims to harness materials to develop functional bone tissue to heal ‘critical-sized’ bone defects. This study examined a robust, coated poly(caprolactone) trimethacrylate (PCL-TMA) 3D-printable scaffold designed to augment bone formation. Following optimisation of the coatings, three bioactive coatings were examined, i) elastin-like polypeptide (ELP), ii) poly(ethyl acrylate) (PEA), fibronectin (FN) and bone morphogenetic protein-2 (BMP-2) applied sequentially (PEA/FN/BMP-2) and iii) both ELP and PEA/FN/BMP-2 coatings applied concurrently. The scaffold material was robust and showed biodegradability. The coatings demonstrated a significant (p < 0.05) osteogenic response in vitro in alkaline phosphatase gene upregulation and alkaline phosphatase production. The PCL-TMA scaffold and coatings supported angiogenesis and displayed excellent biocompatibility following evaluation on the chorioallantoic membrane assay. No significant (p < 0.05) heterotopic bone formed on the scaffolds within a murine subcutaneous implantation model, compared to the positive control of BMP-2 loaded collagen sponge following examination by micro-computed tomography or histology. The current studies demonstrate a range of innovative coated scaffold constructs with in vitro efficacy and clearly illustrate the importance of an appropriate in vivo environment to validate in vitro functionality prior to scale up and preclinical application.https://doi.org/10.1038/s41598-024-75198-3Bioactive coatingBiomaterialBone tissue engineeringCAM assayAnimal models |
| spellingShingle | Karen M. Marshall Jonathan P. Wojciechowski Vineetha Jayawarna Abshar Hasan Cécile Echalier Øystein Øvrebø Tao Yang Kun Zhou Janos M. Kanczler Alvaro Mata Manuel Salmeron-Sanchez Molly M. Stevens Richard O. C. Oreffo Considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivo Scientific Reports Bioactive coating Biomaterial Bone tissue engineering CAM assay Animal models |
| title | Considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivo |
| title_full | Considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivo |
| title_fullStr | Considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivo |
| title_full_unstemmed | Considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivo |
| title_short | Considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivo |
| title_sort | considerations of growth factor and material use in bone tissue engineering using biodegradable scaffolds in vitro and in vivo |
| topic | Bioactive coating Biomaterial Bone tissue engineering CAM assay Animal models |
| url | https://doi.org/10.1038/s41598-024-75198-3 |
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