The Complete Chloroplast Genome Sequence of Callisia fragrans (Lindl.) Woodson (Commelinaceae)

ABSTRACT Callisia fragrans (Lindl.) Woodson (Commelinaceae) is an invasive species in Vietnam but exhibits ornamental and potential medicinal values. However, the genomic data of C. fragrans have not been discovered. In this study, we employed the Illumina sequencing platform to complete the chlorop...

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Main Authors: Khang Vo‐Tan, Van Truong Thi Bich, Men Tran Thanh, Tai Tran Tien, Hoang Dang Khoa Do, Ngoc‐Van Thi Nguyen
Format: Article
Language:English
Published: Wiley 2025-05-01
Series:Ecology and Evolution
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Online Access:https://doi.org/10.1002/ece3.71402
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author Khang Vo‐Tan
Van Truong Thi Bich
Men Tran Thanh
Tai Tran Tien
Hoang Dang Khoa Do
Ngoc‐Van Thi Nguyen
author_facet Khang Vo‐Tan
Van Truong Thi Bich
Men Tran Thanh
Tai Tran Tien
Hoang Dang Khoa Do
Ngoc‐Van Thi Nguyen
author_sort Khang Vo‐Tan
collection DOAJ
description ABSTRACT Callisia fragrans (Lindl.) Woodson (Commelinaceae) is an invasive species in Vietnam but exhibits ornamental and potential medicinal values. However, the genomic data of C. fragrans have not been discovered. In this study, we employed the Illumina sequencing platform to complete the chloroplast genome of C. fragrans, which was 163,887 bp in length. This quadripartite genome consisted of a large single copy region of 90,751 bp, a small single copy region of 18,684 bp, and two inverted repeat regions of 27,226 bp each. Additionally, there were 79 protein‐coding genes, 30 transfer RNA genes, and four ribosomal RNA genes in the chloroplast genome of C. fragrans. Comparative genomic analysis revealed a conserved pattern of genome structure and gene content among Callisia species. However, in contrast to the pseudogenization of accD and rpoA in C. repens and C. insignis, these genes were intact in C. fragrans. Comparative genomic analysis revealed seven variable regions in the chloroplast genomes of three Callisia species, including rps16‐trnQ_UUG, psbI‐trnG_UCC, rpoB‐psbM, trnP_UGG‐rpl33, ndhF‐trnL_UAG, rps15‐ycf1, and ycf1. Phylogenetic analysis indicated the monophyly of Callisia species and a close relationship between C. fragrans and C. repens. This study provides initial data of the chloroplast genome for further genomic studies examining genetic populations, phylogeny, and molecular markers of C. fragrans and related species in Commelinaceae.
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spelling doaj-art-0d3d8213f82f48c387dc355ac8fc7b422025-08-20T02:34:20ZengWileyEcology and Evolution2045-77582025-05-01155n/an/a10.1002/ece3.71402The Complete Chloroplast Genome Sequence of Callisia fragrans (Lindl.) Woodson (Commelinaceae)Khang Vo‐Tan0Van Truong Thi Bich1Men Tran Thanh2Tai Tran Tien3Hoang Dang Khoa Do4Ngoc‐Van Thi Nguyen5Can Tho University Can Tho VietnamCan Tho University Can Tho VietnamCan Tho University Can Tho VietnamPham Ngoc Thach University of Medicine Ho Chi Minh VietnamNTT Hi‐Tech Institute Nguyen Tat Thanh University Ho Chi Minh VietnamCan Tho University of Medicine and Pharmacy Can Tho VietnamABSTRACT Callisia fragrans (Lindl.) Woodson (Commelinaceae) is an invasive species in Vietnam but exhibits ornamental and potential medicinal values. However, the genomic data of C. fragrans have not been discovered. In this study, we employed the Illumina sequencing platform to complete the chloroplast genome of C. fragrans, which was 163,887 bp in length. This quadripartite genome consisted of a large single copy region of 90,751 bp, a small single copy region of 18,684 bp, and two inverted repeat regions of 27,226 bp each. Additionally, there were 79 protein‐coding genes, 30 transfer RNA genes, and four ribosomal RNA genes in the chloroplast genome of C. fragrans. Comparative genomic analysis revealed a conserved pattern of genome structure and gene content among Callisia species. However, in contrast to the pseudogenization of accD and rpoA in C. repens and C. insignis, these genes were intact in C. fragrans. Comparative genomic analysis revealed seven variable regions in the chloroplast genomes of three Callisia species, including rps16‐trnQ_UUG, psbI‐trnG_UCC, rpoB‐psbM, trnP_UGG‐rpl33, ndhF‐trnL_UAG, rps15‐ycf1, and ycf1. Phylogenetic analysis indicated the monophyly of Callisia species and a close relationship between C. fragrans and C. repens. This study provides initial data of the chloroplast genome for further genomic studies examining genetic populations, phylogeny, and molecular markers of C. fragrans and related species in Commelinaceae.https://doi.org/10.1002/ece3.71402Commelinoideaecomparative genomicsinvasive speciesspiderworts
spellingShingle Khang Vo‐Tan
Van Truong Thi Bich
Men Tran Thanh
Tai Tran Tien
Hoang Dang Khoa Do
Ngoc‐Van Thi Nguyen
The Complete Chloroplast Genome Sequence of Callisia fragrans (Lindl.) Woodson (Commelinaceae)
Ecology and Evolution
Commelinoideae
comparative genomics
invasive species
spiderworts
title The Complete Chloroplast Genome Sequence of Callisia fragrans (Lindl.) Woodson (Commelinaceae)
title_full The Complete Chloroplast Genome Sequence of Callisia fragrans (Lindl.) Woodson (Commelinaceae)
title_fullStr The Complete Chloroplast Genome Sequence of Callisia fragrans (Lindl.) Woodson (Commelinaceae)
title_full_unstemmed The Complete Chloroplast Genome Sequence of Callisia fragrans (Lindl.) Woodson (Commelinaceae)
title_short The Complete Chloroplast Genome Sequence of Callisia fragrans (Lindl.) Woodson (Commelinaceae)
title_sort complete chloroplast genome sequence of callisia fragrans lindl woodson commelinaceae
topic Commelinoideae
comparative genomics
invasive species
spiderworts
url https://doi.org/10.1002/ece3.71402
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