Deficient induction response in a Xenopus nucleocytoplasmic hybrid.
Incompatibilities between the nucleus and the cytoplasm of sufficiently distant species result in developmental arrest of hybrid and nucleocytoplasmic hybrid (cybrid) embryos. Several hypotheses have been proposed to explain their lethality, including problems in embryonic genome activation (EGA) an...
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Public Library of Science (PLoS)
2011-11-01
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| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.1001197&type=printable |
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| author | Patrick Narbonne David E Simpson John B Gurdon |
| author_facet | Patrick Narbonne David E Simpson John B Gurdon |
| author_sort | Patrick Narbonne |
| collection | DOAJ |
| description | Incompatibilities between the nucleus and the cytoplasm of sufficiently distant species result in developmental arrest of hybrid and nucleocytoplasmic hybrid (cybrid) embryos. Several hypotheses have been proposed to explain their lethality, including problems in embryonic genome activation (EGA) and/or nucleo-mitochondrial interactions. However, conclusive identification of the causes underlying developmental defects of cybrid embryos is still lacking. We show here that while over 80% of both Xenopus laevis and Xenopus (Silurana) tropicalis same-species androgenetic haploids develop to the swimming tadpole stage, the androgenetic cybrids formed by the combination of X. laevis egg cytoplasm and X. tropicalis sperm nucleus invariably fail to gastrulate properly and never reach the swimming tadpole stage. In spite of this arrest, these cybrids show quantitatively normal EGA and energy levels at the stage where their initial gastrulation defects are manifested. The nucleocytoplasmic incompatibility between these two species instead results from a combination of factors, including a reduced emission of induction signal from the vegetal half, a decreased sensitivity of animal cells to induction signals, and differences in a key embryonic protein (Xbra) concentration between the two species, together leading to inefficient induction and defective convergence-extension during gastrulation. Indeed, increased exposure to induction signals and/or Xbra signalling partially rescues the induction response in animal explants and whole cybrid embryos. Altogether, our study demonstrates that the egg cytoplasm of one species may not support the development promoted by the nucleus of another species, even if this nucleus does not interfere with the cytoplasmic/maternal functions of the egg, while the egg cytoplasm is also capable of activating the genome of that nucleus. Instead, our results provide evidence that inefficient signalling and differences in the concentrations of key proteins between species lead to developmental defects in cybrids. Finally, they show that the incompatibilities of cybrids can be corrected by appropriate treatments. |
| format | Article |
| id | doaj-art-0d3a4e9484934e51af03556f98f329a0 |
| institution | DOAJ |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2011-11-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Biology |
| spelling | doaj-art-0d3a4e9484934e51af03556f98f329a02025-08-20T03:10:07ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852011-11-01911e100119710.1371/journal.pbio.1001197Deficient induction response in a Xenopus nucleocytoplasmic hybrid.Patrick NarbonneDavid E SimpsonJohn B GurdonIncompatibilities between the nucleus and the cytoplasm of sufficiently distant species result in developmental arrest of hybrid and nucleocytoplasmic hybrid (cybrid) embryos. Several hypotheses have been proposed to explain their lethality, including problems in embryonic genome activation (EGA) and/or nucleo-mitochondrial interactions. However, conclusive identification of the causes underlying developmental defects of cybrid embryos is still lacking. We show here that while over 80% of both Xenopus laevis and Xenopus (Silurana) tropicalis same-species androgenetic haploids develop to the swimming tadpole stage, the androgenetic cybrids formed by the combination of X. laevis egg cytoplasm and X. tropicalis sperm nucleus invariably fail to gastrulate properly and never reach the swimming tadpole stage. In spite of this arrest, these cybrids show quantitatively normal EGA and energy levels at the stage where their initial gastrulation defects are manifested. The nucleocytoplasmic incompatibility between these two species instead results from a combination of factors, including a reduced emission of induction signal from the vegetal half, a decreased sensitivity of animal cells to induction signals, and differences in a key embryonic protein (Xbra) concentration between the two species, together leading to inefficient induction and defective convergence-extension during gastrulation. Indeed, increased exposure to induction signals and/or Xbra signalling partially rescues the induction response in animal explants and whole cybrid embryos. Altogether, our study demonstrates that the egg cytoplasm of one species may not support the development promoted by the nucleus of another species, even if this nucleus does not interfere with the cytoplasmic/maternal functions of the egg, while the egg cytoplasm is also capable of activating the genome of that nucleus. Instead, our results provide evidence that inefficient signalling and differences in the concentrations of key proteins between species lead to developmental defects in cybrids. Finally, they show that the incompatibilities of cybrids can be corrected by appropriate treatments.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.1001197&type=printable |
| spellingShingle | Patrick Narbonne David E Simpson John B Gurdon Deficient induction response in a Xenopus nucleocytoplasmic hybrid. PLoS Biology |
| title | Deficient induction response in a Xenopus nucleocytoplasmic hybrid. |
| title_full | Deficient induction response in a Xenopus nucleocytoplasmic hybrid. |
| title_fullStr | Deficient induction response in a Xenopus nucleocytoplasmic hybrid. |
| title_full_unstemmed | Deficient induction response in a Xenopus nucleocytoplasmic hybrid. |
| title_short | Deficient induction response in a Xenopus nucleocytoplasmic hybrid. |
| title_sort | deficient induction response in a xenopus nucleocytoplasmic hybrid |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.1001197&type=printable |
| work_keys_str_mv | AT patricknarbonne deficientinductionresponseinaxenopusnucleocytoplasmichybrid AT davidesimpson deficientinductionresponseinaxenopusnucleocytoplasmichybrid AT johnbgurdon deficientinductionresponseinaxenopusnucleocytoplasmichybrid |