Development of transgenic wheat plants withstand salt stress via the MDAR1 gene
In light of the fact that climate change has emerged as one of the difficulties confronting the global food system, researchers are obligated to work toward developing fundamental crops, particularly wheat, to combat environmental stress, including drought and salt. In the present study, genetic eng...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2025-12-01
|
| Series: | GM Crops & Food |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21645698.2025.2463139 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850255830438379520 |
|---|---|
| author | Mohamed Abdelsattar Ahmed M. Ramadan Amin E. Eltayeb Osama M. Saleh Fatthy M. Abdel-Tawab Eman M. Fahmy Sameh E. Hassanein Hani M. Ali Najla B. S. Al-Saud Hussien. F. Alameldin Sabah M. Hassan Nermin G. Mohamed Ahmed Z. Abdel Azeiz Ahmed Bahieldin Hala F. Eissa |
| author_facet | Mohamed Abdelsattar Ahmed M. Ramadan Amin E. Eltayeb Osama M. Saleh Fatthy M. Abdel-Tawab Eman M. Fahmy Sameh E. Hassanein Hani M. Ali Najla B. S. Al-Saud Hussien. F. Alameldin Sabah M. Hassan Nermin G. Mohamed Ahmed Z. Abdel Azeiz Ahmed Bahieldin Hala F. Eissa |
| author_sort | Mohamed Abdelsattar |
| collection | DOAJ |
| description | In light of the fact that climate change has emerged as one of the difficulties confronting the global food system, researchers are obligated to work toward developing fundamental crops, particularly wheat, to combat environmental stress, including drought and salt. In the present study, genetic engineering was used to transfer the Arabidopsis MDAR1 gene, which controls the buildup of ascorbic acid (AsA) to make bread wheat less likely to be sensitive to salt stress. The biolistic bombardment was used to transfer cDNA from the Arabidopsis thaliana plant that encodes MDAR1 into Bobwhite 56 cultivar wheat plants. A molecular investigation was performed on six different transgenic lines to confirm the integration of the transgene, the copy number, and the expression of the transgene. There were one to three copies of the transgene, and there was no association found between the number of copies of the transgene and All the data generated or analyzed during this study are included in this published article [and its supplementary information files].the presence of its expression. Compared to plants that were not transgenic, the amount of ascorbic acid (AsA) that accumulated in the transgenic plants was twice as high. ROS concentrations are significantly lower in transgenic plants compared to non-transgenic plants under both control and salt stress conditions, effectively reducing oxidative stress. By cultivating transgenic T2 plants in a greenhouse, we were able to determine whether they were able to tolerate the potentially damaging effects of salt stress (200 mm). The study concluded that transgenic wheat plants that consistently expressed the MDAR1 gene become tolerant to salt stress with improvement in growth characteristics. |
| format | Article |
| id | doaj-art-0d39e623c4f348c485ce960c4126d943 |
| institution | OA Journals |
| issn | 2164-5698 2164-5701 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | GM Crops & Food |
| spelling | doaj-art-0d39e623c4f348c485ce960c4126d9432025-08-20T01:56:46ZengTaylor & Francis GroupGM Crops & Food2164-56982164-57012025-12-0116117318710.1080/21645698.2025.2463139Development of transgenic wheat plants withstand salt stress via the MDAR1 geneMohamed Abdelsattar0Ahmed M. Ramadan1Amin E. Eltayeb2Osama M. Saleh3Fatthy M. Abdel-Tawab4Eman M. Fahmy5Sameh E. Hassanein6Hani M. Ali7Najla B. S. Al-Saud8Hussien. F. Alameldin9Sabah M. Hassan10Nermin G. Mohamed11Ahmed Z. Abdel Azeiz12Ahmed Bahieldin13Hala F. Eissa14Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, EgyptDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi ArabiaArid Land Research Center, Tottori University, Tottori, JapanDepartment of Natural Products Research, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy AuthorityDepartment of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, EgyptDepartment of Genetics, Faculty of Agriculture, Ain Shams University, Cairo, EgyptAgricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, EgyptDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi ArabiaDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi ArabiaAgricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, EgyptDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi ArabiaCollege of Biotechnology, Misr University for Science and TechnologyCollege of Biotechnology, Misr University for Science and TechnologyDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi ArabiaAgricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, EgyptIn light of the fact that climate change has emerged as one of the difficulties confronting the global food system, researchers are obligated to work toward developing fundamental crops, particularly wheat, to combat environmental stress, including drought and salt. In the present study, genetic engineering was used to transfer the Arabidopsis MDAR1 gene, which controls the buildup of ascorbic acid (AsA) to make bread wheat less likely to be sensitive to salt stress. The biolistic bombardment was used to transfer cDNA from the Arabidopsis thaliana plant that encodes MDAR1 into Bobwhite 56 cultivar wheat plants. A molecular investigation was performed on six different transgenic lines to confirm the integration of the transgene, the copy number, and the expression of the transgene. There were one to three copies of the transgene, and there was no association found between the number of copies of the transgene and All the data generated or analyzed during this study are included in this published article [and its supplementary information files].the presence of its expression. Compared to plants that were not transgenic, the amount of ascorbic acid (AsA) that accumulated in the transgenic plants was twice as high. ROS concentrations are significantly lower in transgenic plants compared to non-transgenic plants under both control and salt stress conditions, effectively reducing oxidative stress. By cultivating transgenic T2 plants in a greenhouse, we were able to determine whether they were able to tolerate the potentially damaging effects of salt stress (200 mm). The study concluded that transgenic wheat plants that consistently expressed the MDAR1 gene become tolerant to salt stress with improvement in growth characteristics.https://www.tandfonline.com/doi/10.1080/21645698.2025.2463139Wheat transformationMDAR1 geneAsAsalt stress |
| spellingShingle | Mohamed Abdelsattar Ahmed M. Ramadan Amin E. Eltayeb Osama M. Saleh Fatthy M. Abdel-Tawab Eman M. Fahmy Sameh E. Hassanein Hani M. Ali Najla B. S. Al-Saud Hussien. F. Alameldin Sabah M. Hassan Nermin G. Mohamed Ahmed Z. Abdel Azeiz Ahmed Bahieldin Hala F. Eissa Development of transgenic wheat plants withstand salt stress via the MDAR1 gene GM Crops & Food Wheat transformation MDAR1 gene AsA salt stress |
| title | Development of transgenic wheat plants withstand salt stress via the MDAR1 gene |
| title_full | Development of transgenic wheat plants withstand salt stress via the MDAR1 gene |
| title_fullStr | Development of transgenic wheat plants withstand salt stress via the MDAR1 gene |
| title_full_unstemmed | Development of transgenic wheat plants withstand salt stress via the MDAR1 gene |
| title_short | Development of transgenic wheat plants withstand salt stress via the MDAR1 gene |
| title_sort | development of transgenic wheat plants withstand salt stress via the mdar1 gene |
| topic | Wheat transformation MDAR1 gene AsA salt stress |
| url | https://www.tandfonline.com/doi/10.1080/21645698.2025.2463139 |
| work_keys_str_mv | AT mohamedabdelsattar developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT ahmedmramadan developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT amineeltayeb developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT osamamsaleh developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT fatthymabdeltawab developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT emanmfahmy developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT samehehassanein developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT hanimali developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT najlabsalsaud developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT hussienfalameldin developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT sabahmhassan developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT nermingmohamed developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT ahmedzabdelazeiz developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT ahmedbahieldin developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene AT halafeissa developmentoftransgenicwheatplantswithstandsaltstressviathemdar1gene |