Acoustic loudness factor as an experimental parameter for benchmarking small molecule photoacoustic probes
Abstract Photoacoustic imaging (PAI) is an emerging biomedical imaging modality with promise as a point-of-care diagnostic. This imaging modality relies on optical excitation of an absorber followed by production of ultrasound through the photoacoustic effect, resulting in high spatial resolution wi...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-04-01
|
| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-59121-6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850170777498812416 |
|---|---|
| author | Frederik Brøndsted Julia L. McAfee Jerimiah D. Moore Harry R. Shield Luca Menozzi Xinqi Zhou Yuan Fang Ruwen Yin Junjie Yao Kelsey P. Kubelick Cliff I. Stains |
| author_facet | Frederik Brøndsted Julia L. McAfee Jerimiah D. Moore Harry R. Shield Luca Menozzi Xinqi Zhou Yuan Fang Ruwen Yin Junjie Yao Kelsey P. Kubelick Cliff I. Stains |
| author_sort | Frederik Brøndsted |
| collection | DOAJ |
| description | Abstract Photoacoustic imaging (PAI) is an emerging biomedical imaging modality with promise as a point-of-care diagnostic. This imaging modality relies on optical excitation of an absorber followed by production of ultrasound through the photoacoustic effect, resulting in high spatial resolution with imaging depths in the centimeter range. Herein, we disclose the discovery of the first benchmarking parameter for small molecule dye performance in PAI, which we term the acoustic loudness factor (ALF). ALF can predict dye performance in PAI without the need for access to photoacoustic instrumentation and can be used to guide the systematic evaluation of design strategies to enhance photoacoustic signal. Lastly, we demonstrate that enhancements in ALF can be translated to in vivo PAI. Akin to the use of fluorescence brightness in fluorophore design and evaluation for fluorescence imaging, we anticipate that ALF will guide the design and evaluation of improved probes for PAI. |
| format | Article |
| id | doaj-art-0d15c45fb37c468dac7ad36702f8ce72 |
| institution | OA Journals |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-0d15c45fb37c468dac7ad36702f8ce722025-08-20T02:20:25ZengNature PortfolioNature Communications2041-17232025-04-0116111210.1038/s41467-025-59121-6Acoustic loudness factor as an experimental parameter for benchmarking small molecule photoacoustic probesFrederik Brøndsted0Julia L. McAfee1Jerimiah D. Moore2Harry R. Shield3Luca Menozzi4Xinqi Zhou5Yuan Fang6Ruwen Yin7Junjie Yao8Kelsey P. Kubelick9Cliff I. Stains10Department of Chemistry, University of VirginiaDepartment of Chemistry, University of VirginiaDepartment of Chemistry, University of VirginiaDepartment of Chemistry, University of VirginiaDepartment of Biomedical Engineering, Duke UniversityDepartment of Chemistry, University of Nebraska-LincolnDepartment of Chemistry, University of VirginiaDepartment of Chemistry, University of VirginiaDepartment of Biomedical Engineering, Duke UniversityDepartment of Biomedical Engineering, University of VirginiaDepartment of Chemistry, University of VirginiaAbstract Photoacoustic imaging (PAI) is an emerging biomedical imaging modality with promise as a point-of-care diagnostic. This imaging modality relies on optical excitation of an absorber followed by production of ultrasound through the photoacoustic effect, resulting in high spatial resolution with imaging depths in the centimeter range. Herein, we disclose the discovery of the first benchmarking parameter for small molecule dye performance in PAI, which we term the acoustic loudness factor (ALF). ALF can predict dye performance in PAI without the need for access to photoacoustic instrumentation and can be used to guide the systematic evaluation of design strategies to enhance photoacoustic signal. Lastly, we demonstrate that enhancements in ALF can be translated to in vivo PAI. Akin to the use of fluorescence brightness in fluorophore design and evaluation for fluorescence imaging, we anticipate that ALF will guide the design and evaluation of improved probes for PAI.https://doi.org/10.1038/s41467-025-59121-6 |
| spellingShingle | Frederik Brøndsted Julia L. McAfee Jerimiah D. Moore Harry R. Shield Luca Menozzi Xinqi Zhou Yuan Fang Ruwen Yin Junjie Yao Kelsey P. Kubelick Cliff I. Stains Acoustic loudness factor as an experimental parameter for benchmarking small molecule photoacoustic probes Nature Communications |
| title | Acoustic loudness factor as an experimental parameter for benchmarking small molecule photoacoustic probes |
| title_full | Acoustic loudness factor as an experimental parameter for benchmarking small molecule photoacoustic probes |
| title_fullStr | Acoustic loudness factor as an experimental parameter for benchmarking small molecule photoacoustic probes |
| title_full_unstemmed | Acoustic loudness factor as an experimental parameter for benchmarking small molecule photoacoustic probes |
| title_short | Acoustic loudness factor as an experimental parameter for benchmarking small molecule photoacoustic probes |
| title_sort | acoustic loudness factor as an experimental parameter for benchmarking small molecule photoacoustic probes |
| url | https://doi.org/10.1038/s41467-025-59121-6 |
| work_keys_str_mv | AT frederikbrøndsted acousticloudnessfactorasanexperimentalparameterforbenchmarkingsmallmoleculephotoacousticprobes AT julialmcafee acousticloudnessfactorasanexperimentalparameterforbenchmarkingsmallmoleculephotoacousticprobes AT jerimiahdmoore acousticloudnessfactorasanexperimentalparameterforbenchmarkingsmallmoleculephotoacousticprobes AT harryrshield acousticloudnessfactorasanexperimentalparameterforbenchmarkingsmallmoleculephotoacousticprobes AT lucamenozzi acousticloudnessfactorasanexperimentalparameterforbenchmarkingsmallmoleculephotoacousticprobes AT xinqizhou acousticloudnessfactorasanexperimentalparameterforbenchmarkingsmallmoleculephotoacousticprobes AT yuanfang acousticloudnessfactorasanexperimentalparameterforbenchmarkingsmallmoleculephotoacousticprobes AT ruwenyin acousticloudnessfactorasanexperimentalparameterforbenchmarkingsmallmoleculephotoacousticprobes AT junjieyao acousticloudnessfactorasanexperimentalparameterforbenchmarkingsmallmoleculephotoacousticprobes AT kelseypkubelick acousticloudnessfactorasanexperimentalparameterforbenchmarkingsmallmoleculephotoacousticprobes AT cliffistains acousticloudnessfactorasanexperimentalparameterforbenchmarkingsmallmoleculephotoacousticprobes |