Longitudinal Analysis of Natural Killer Cells in Dengue Virus-Infected Patients in Comparison to Chikungunya and Chikungunya/Dengue Virus-Infected Patients.
<h4>Background</h4>Dengue virus (DENV) is the most prominent arbovirus worldwide, causing major epidemics in South-East Asia, South America and Africa. In 2010, a major DENV-2 outbreak occurred in Gabon with cases of patients co-infected with chikungunya virus (CHIKV). Although the innat...
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Public Library of Science (PLoS)
2016-03-01
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| Series: | PLoS Neglected Tropical Diseases |
| Online Access: | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0004499&type=printable |
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| author | Caroline Petitdemange Nadia Wauquier Hervé Devilliers Hans Yssel Illich Mombo Mélanie Caron Dieudonné Nkoghé Patrice Debré Eric Leroy Vincent Vieillard |
| author_facet | Caroline Petitdemange Nadia Wauquier Hervé Devilliers Hans Yssel Illich Mombo Mélanie Caron Dieudonné Nkoghé Patrice Debré Eric Leroy Vincent Vieillard |
| author_sort | Caroline Petitdemange |
| collection | DOAJ |
| description | <h4>Background</h4>Dengue virus (DENV) is the most prominent arbovirus worldwide, causing major epidemics in South-East Asia, South America and Africa. In 2010, a major DENV-2 outbreak occurred in Gabon with cases of patients co-infected with chikungunya virus (CHIKV). Although the innate immune response is thought to be of primordial importance in the development and outcome of arbovirus-associated pathologies, our knowledge of the role of natural killer (NK) cells during DENV-2 infection is in its infancy.<h4>Methodology</h4>We performed the first extensive comparative longitudinal characterization of NK cells in patients infected by DENV-2, CHIKV or both viruses. Hierarchical clustering and principal component analyses were performed to discriminate between CHIKV and DENV-2 infected patients.<h4>Principal findings</h4>We observed that both activation and differentiation of NK cells are induced during the acute phase of infection by DENV-2 and CHIKV. Combinatorial analysis however, revealed that both arboviruses induced two different signatures of NK-cell responses, with CHIKV more associated with terminal differentiation, and DENV-2 with inhibitory KIRs. We show also that intracellular production of interferon-γ (IFN-γ) by NK cells is strongly stimulated in acute DENV-2 infection, compared to CHIKV.<h4>Conclusions/significance</h4>Although specific differences were observed between CHIKV and DENV-2 infections, the significant remodeling of NK cell populations observed here suggests their potential roles in the control of both infections. |
| format | Article |
| id | doaj-art-0d100b20103c43fe833ddb213bf53564 |
| institution | DOAJ |
| issn | 1935-2727 1935-2735 |
| language | English |
| publishDate | 2016-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Neglected Tropical Diseases |
| spelling | doaj-art-0d100b20103c43fe833ddb213bf535642025-08-20T03:11:11ZengPublic Library of Science (PLoS)PLoS Neglected Tropical Diseases1935-27271935-27352016-03-01103e000449910.1371/journal.pntd.0004499Longitudinal Analysis of Natural Killer Cells in Dengue Virus-Infected Patients in Comparison to Chikungunya and Chikungunya/Dengue Virus-Infected Patients.Caroline PetitdemangeNadia WauquierHervé DevilliersHans YsselIllich MomboMélanie CaronDieudonné NkoghéPatrice DebréEric LeroyVincent Vieillard<h4>Background</h4>Dengue virus (DENV) is the most prominent arbovirus worldwide, causing major epidemics in South-East Asia, South America and Africa. In 2010, a major DENV-2 outbreak occurred in Gabon with cases of patients co-infected with chikungunya virus (CHIKV). Although the innate immune response is thought to be of primordial importance in the development and outcome of arbovirus-associated pathologies, our knowledge of the role of natural killer (NK) cells during DENV-2 infection is in its infancy.<h4>Methodology</h4>We performed the first extensive comparative longitudinal characterization of NK cells in patients infected by DENV-2, CHIKV or both viruses. Hierarchical clustering and principal component analyses were performed to discriminate between CHIKV and DENV-2 infected patients.<h4>Principal findings</h4>We observed that both activation and differentiation of NK cells are induced during the acute phase of infection by DENV-2 and CHIKV. Combinatorial analysis however, revealed that both arboviruses induced two different signatures of NK-cell responses, with CHIKV more associated with terminal differentiation, and DENV-2 with inhibitory KIRs. We show also that intracellular production of interferon-γ (IFN-γ) by NK cells is strongly stimulated in acute DENV-2 infection, compared to CHIKV.<h4>Conclusions/significance</h4>Although specific differences were observed between CHIKV and DENV-2 infections, the significant remodeling of NK cell populations observed here suggests their potential roles in the control of both infections.https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0004499&type=printable |
| spellingShingle | Caroline Petitdemange Nadia Wauquier Hervé Devilliers Hans Yssel Illich Mombo Mélanie Caron Dieudonné Nkoghé Patrice Debré Eric Leroy Vincent Vieillard Longitudinal Analysis of Natural Killer Cells in Dengue Virus-Infected Patients in Comparison to Chikungunya and Chikungunya/Dengue Virus-Infected Patients. PLoS Neglected Tropical Diseases |
| title | Longitudinal Analysis of Natural Killer Cells in Dengue Virus-Infected Patients in Comparison to Chikungunya and Chikungunya/Dengue Virus-Infected Patients. |
| title_full | Longitudinal Analysis of Natural Killer Cells in Dengue Virus-Infected Patients in Comparison to Chikungunya and Chikungunya/Dengue Virus-Infected Patients. |
| title_fullStr | Longitudinal Analysis of Natural Killer Cells in Dengue Virus-Infected Patients in Comparison to Chikungunya and Chikungunya/Dengue Virus-Infected Patients. |
| title_full_unstemmed | Longitudinal Analysis of Natural Killer Cells in Dengue Virus-Infected Patients in Comparison to Chikungunya and Chikungunya/Dengue Virus-Infected Patients. |
| title_short | Longitudinal Analysis of Natural Killer Cells in Dengue Virus-Infected Patients in Comparison to Chikungunya and Chikungunya/Dengue Virus-Infected Patients. |
| title_sort | longitudinal analysis of natural killer cells in dengue virus infected patients in comparison to chikungunya and chikungunya dengue virus infected patients |
| url | https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0004499&type=printable |
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