Estrogen-Related Receptor Potential Target Genes in Silkworm (<i>Bombyx mori</i>): Insights into Metabolic Regulation
Estrogen-related receptors (ERRs) are important transcription factors within the nuclear receptor family that regulate cellular energy storage and consumption by binding to estrogen-related receptor response elements (ERREs) on gene promoters. While ERRs’ role in vertebrates is well-studied, their m...
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2025-04-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/16/5/469 |
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| author | Luyu Hou Jinxin Wu Die Liu Haoran Xu Hongbo Yao Yiwen Liang Qingyou Xia Ping Lin Guanwang Shen |
| author_facet | Luyu Hou Jinxin Wu Die Liu Haoran Xu Hongbo Yao Yiwen Liang Qingyou Xia Ping Lin Guanwang Shen |
| author_sort | Luyu Hou |
| collection | DOAJ |
| description | Estrogen-related receptors (ERRs) are important transcription factors within the nuclear receptor family that regulate cellular energy storage and consumption by binding to estrogen-related receptor response elements (ERREs) on gene promoters. While ERRs’ role in vertebrates is well-studied, their molecular mechanisms in insect metabolism and development remain unclear. This study systematically summarizes the functions of ERRs in insects, focusing on silkworms by analyzing gene functions and comparing databases. ERRE-like elements were identified in the 2000 bp upstream promoter regions of 69 metabolism-related silkworm genes. Furthermore, electrophoretic mobility shift assays (EMSAs) revealed that ERREs within the promoters of 15 genes related to sugar, fat, and protein metabolism specifically bind to ERR. Notably, an ERRE in the promoter of the trehalose transporter 1 gene (<i>BmTret1</i>), crucial for trehalose homeostasis in insect hemolymph, exhibited significantly enhanced activity in ERR-overexpressing cells. These findings suggest that ERR is a potential regulatory factor in silkworm metabolism and refine its metabolic regulatory network. This study highlights the broader and more critical role of ERR in insects than that previously recognized, contributing to a deeper understanding of insect metabolism and its potential applications in related fields. |
| format | Article |
| id | doaj-art-0d0ae6afceef44ecb4e5258751a1af67 |
| institution | OA Journals |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj-art-0d0ae6afceef44ecb4e5258751a1af672025-08-20T01:56:19ZengMDPI AGInsects2075-44502025-04-0116546910.3390/insects16050469Estrogen-Related Receptor Potential Target Genes in Silkworm (<i>Bombyx mori</i>): Insights into Metabolic RegulationLuyu Hou0Jinxin Wu1Die Liu2Haoran Xu3Hongbo Yao4Yiwen Liang5Qingyou Xia6Ping Lin7Guanwang Shen8Biological Science Research Center, Southwest University, Chongqing 400715, ChinaBiological Science Research Center, Southwest University, Chongqing 400715, ChinaBiological Science Research Center, Southwest University, Chongqing 400715, ChinaBiological Science Research Center, Southwest University, Chongqing 400715, ChinaBiological Science Research Center, Southwest University, Chongqing 400715, ChinaCollege of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, ChinaBiological Science Research Center, Southwest University, Chongqing 400715, ChinaBiological Science Research Center, Southwest University, Chongqing 400715, ChinaBiological Science Research Center, Southwest University, Chongqing 400715, ChinaEstrogen-related receptors (ERRs) are important transcription factors within the nuclear receptor family that regulate cellular energy storage and consumption by binding to estrogen-related receptor response elements (ERREs) on gene promoters. While ERRs’ role in vertebrates is well-studied, their molecular mechanisms in insect metabolism and development remain unclear. This study systematically summarizes the functions of ERRs in insects, focusing on silkworms by analyzing gene functions and comparing databases. ERRE-like elements were identified in the 2000 bp upstream promoter regions of 69 metabolism-related silkworm genes. Furthermore, electrophoretic mobility shift assays (EMSAs) revealed that ERREs within the promoters of 15 genes related to sugar, fat, and protein metabolism specifically bind to ERR. Notably, an ERRE in the promoter of the trehalose transporter 1 gene (<i>BmTret1</i>), crucial for trehalose homeostasis in insect hemolymph, exhibited significantly enhanced activity in ERR-overexpressing cells. These findings suggest that ERR is a potential regulatory factor in silkworm metabolism and refine its metabolic regulatory network. This study highlights the broader and more critical role of ERR in insects than that previously recognized, contributing to a deeper understanding of insect metabolism and its potential applications in related fields.https://www.mdpi.com/2075-4450/16/5/469estrogen-related receptorestrogen-related receptor response elementmetabolic regulationphysiological functionsilkworm |
| spellingShingle | Luyu Hou Jinxin Wu Die Liu Haoran Xu Hongbo Yao Yiwen Liang Qingyou Xia Ping Lin Guanwang Shen Estrogen-Related Receptor Potential Target Genes in Silkworm (<i>Bombyx mori</i>): Insights into Metabolic Regulation Insects estrogen-related receptor estrogen-related receptor response element metabolic regulation physiological function silkworm |
| title | Estrogen-Related Receptor Potential Target Genes in Silkworm (<i>Bombyx mori</i>): Insights into Metabolic Regulation |
| title_full | Estrogen-Related Receptor Potential Target Genes in Silkworm (<i>Bombyx mori</i>): Insights into Metabolic Regulation |
| title_fullStr | Estrogen-Related Receptor Potential Target Genes in Silkworm (<i>Bombyx mori</i>): Insights into Metabolic Regulation |
| title_full_unstemmed | Estrogen-Related Receptor Potential Target Genes in Silkworm (<i>Bombyx mori</i>): Insights into Metabolic Regulation |
| title_short | Estrogen-Related Receptor Potential Target Genes in Silkworm (<i>Bombyx mori</i>): Insights into Metabolic Regulation |
| title_sort | estrogen related receptor potential target genes in silkworm i bombyx mori i insights into metabolic regulation |
| topic | estrogen-related receptor estrogen-related receptor response element metabolic regulation physiological function silkworm |
| url | https://www.mdpi.com/2075-4450/16/5/469 |
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