Exploring the therapeutic effects of Ulvan species (Ulva fasciata and Ulva rigida) on hyperglycemia, hyperlipidemia and adipose oxidative injury
This study elucidated the protective effects of two Ulvan seaweed species, Ulva rigida and Ulva fasciata on oxidative stress, obesity and diabetes related parameters, using in vitro, and ex vivo experimental models. Dried whole U. rigida and U. fasciata were used to obtain the crude aqueous and etha...
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Elsevier
2025-03-01
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| Series: | Scientific African |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468227624004605 |
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| author | S'thandiwe N. Magwaza Veronica F. Salau Kolawole A. Olofinsan Md. Shahidul Islam |
| author_facet | S'thandiwe N. Magwaza Veronica F. Salau Kolawole A. Olofinsan Md. Shahidul Islam |
| author_sort | S'thandiwe N. Magwaza |
| collection | DOAJ |
| description | This study elucidated the protective effects of two Ulvan seaweed species, Ulva rigida and Ulva fasciata on oxidative stress, obesity and diabetes related parameters, using in vitro, and ex vivo experimental models. Dried whole U. rigida and U. fasciata were used to obtain the crude aqueous and ethanol extracts. The in vitro studies were used to evaluate the effects of the seaweed extracts on key lipid and carbohydrate digestive enzymes including α- glucosidase, α- amylase and pancreatic lipase. For ex vivo studies, the intestinal glucose absorption inhibitory activities as well as muscle glucose uptake stimulating activities of the extracts were assessed. Furthermore, for antioxidant activities, the oxidative damage was induced by incubating the adipose tissue with 0.1 M ferrous sulphate (FeSO4), and then treated with the crude aqueous and ethanol extracts of Ulva rigida and Ulva fasciata for 30 min at 37°C. All the experiments were conducted using established methods. The aqueous extract of Ulva rigida exhibited strongest α- amylase and α- glucosidase inhibitory activities (IC50 96.29 µg/ml and 93.56 µg/ml), while the highest pancreatic lipase inhibition was depicted by the aqueous extract of Ulva fasciata (IC50 184.35 µg/ml). The extracts significantly (P < 0.05) decreased intestinal glucose absorption and promoted glucose uptake by the muscle. Moreover, following treatment of oxidatively damaged adipose with Ulva rigida and Ulva fasciata extracts, there was decreased levels of lipid peroxidation and increased glutathione peroxidase, superoxide dismutase, and catalase activities. The aqueous and ethanol extracts of Ulva rigida and Ulva fasciata revealed the presence of flavonoids and phenols when screened under Liquid Chromatogram Mass spectrometry (LCMS). Both Ulvan species demonstrated anti-hyperglycaemic and anti-hyperlipidaemic effects in a dose dependant manner. Further in vivo experimental studies are required to substantiate the present results. Additionally, future research on isolated compounds from the seaweed extracts that could be responsible for these observed effects are required towards the development of anti-diabetic and anti-obesity drugs. |
| format | Article |
| id | doaj-art-0d075d544853420da1f5e5ba1b870128 |
| institution | DOAJ |
| issn | 2468-2276 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Scientific African |
| spelling | doaj-art-0d075d544853420da1f5e5ba1b8701282025-08-20T03:01:42ZengElsevierScientific African2468-22762025-03-0127e0251810.1016/j.sciaf.2024.e02518Exploring the therapeutic effects of Ulvan species (Ulva fasciata and Ulva rigida) on hyperglycemia, hyperlipidemia and adipose oxidative injuryS'thandiwe N. Magwaza0Veronica F. Salau1Kolawole A. Olofinsan2Md. Shahidul Islam3Department of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 4000, South AfricaDepartment of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 4000, South Africa; Department of Pharmacology, University of the Free State, Bloemfontein 9300, South AfricaDepartment of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 4000, South Africa; Department of Pharmacology, University of the Free State, Bloemfontein 9300, South AfricaDepartment of Biochemistry, School of Life Sciences, University of KwaZulu-Natal, Westville Campus, Durban 4000, South Africa; Corresponding author.This study elucidated the protective effects of two Ulvan seaweed species, Ulva rigida and Ulva fasciata on oxidative stress, obesity and diabetes related parameters, using in vitro, and ex vivo experimental models. Dried whole U. rigida and U. fasciata were used to obtain the crude aqueous and ethanol extracts. The in vitro studies were used to evaluate the effects of the seaweed extracts on key lipid and carbohydrate digestive enzymes including α- glucosidase, α- amylase and pancreatic lipase. For ex vivo studies, the intestinal glucose absorption inhibitory activities as well as muscle glucose uptake stimulating activities of the extracts were assessed. Furthermore, for antioxidant activities, the oxidative damage was induced by incubating the adipose tissue with 0.1 M ferrous sulphate (FeSO4), and then treated with the crude aqueous and ethanol extracts of Ulva rigida and Ulva fasciata for 30 min at 37°C. All the experiments were conducted using established methods. The aqueous extract of Ulva rigida exhibited strongest α- amylase and α- glucosidase inhibitory activities (IC50 96.29 µg/ml and 93.56 µg/ml), while the highest pancreatic lipase inhibition was depicted by the aqueous extract of Ulva fasciata (IC50 184.35 µg/ml). The extracts significantly (P < 0.05) decreased intestinal glucose absorption and promoted glucose uptake by the muscle. Moreover, following treatment of oxidatively damaged adipose with Ulva rigida and Ulva fasciata extracts, there was decreased levels of lipid peroxidation and increased glutathione peroxidase, superoxide dismutase, and catalase activities. The aqueous and ethanol extracts of Ulva rigida and Ulva fasciata revealed the presence of flavonoids and phenols when screened under Liquid Chromatogram Mass spectrometry (LCMS). Both Ulvan species demonstrated anti-hyperglycaemic and anti-hyperlipidaemic effects in a dose dependant manner. Further in vivo experimental studies are required to substantiate the present results. Additionally, future research on isolated compounds from the seaweed extracts that could be responsible for these observed effects are required towards the development of anti-diabetic and anti-obesity drugs.http://www.sciencedirect.com/science/article/pii/S2468227624004605Intestinal glucose absorptionKey digestive enzymesOxidative stressSeaweedsUlvan rigidaUlvan fasciata |
| spellingShingle | S'thandiwe N. Magwaza Veronica F. Salau Kolawole A. Olofinsan Md. Shahidul Islam Exploring the therapeutic effects of Ulvan species (Ulva fasciata and Ulva rigida) on hyperglycemia, hyperlipidemia and adipose oxidative injury Scientific African Intestinal glucose absorption Key digestive enzymes Oxidative stress Seaweeds Ulvan rigida Ulvan fasciata |
| title | Exploring the therapeutic effects of Ulvan species (Ulva fasciata and Ulva rigida) on hyperglycemia, hyperlipidemia and adipose oxidative injury |
| title_full | Exploring the therapeutic effects of Ulvan species (Ulva fasciata and Ulva rigida) on hyperglycemia, hyperlipidemia and adipose oxidative injury |
| title_fullStr | Exploring the therapeutic effects of Ulvan species (Ulva fasciata and Ulva rigida) on hyperglycemia, hyperlipidemia and adipose oxidative injury |
| title_full_unstemmed | Exploring the therapeutic effects of Ulvan species (Ulva fasciata and Ulva rigida) on hyperglycemia, hyperlipidemia and adipose oxidative injury |
| title_short | Exploring the therapeutic effects of Ulvan species (Ulva fasciata and Ulva rigida) on hyperglycemia, hyperlipidemia and adipose oxidative injury |
| title_sort | exploring the therapeutic effects of ulvan species ulva fasciata and ulva rigida on hyperglycemia hyperlipidemia and adipose oxidative injury |
| topic | Intestinal glucose absorption Key digestive enzymes Oxidative stress Seaweeds Ulvan rigida Ulvan fasciata |
| url | http://www.sciencedirect.com/science/article/pii/S2468227624004605 |
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