Influence of Liquid Level Height on Flow Characteristics and Mixing Efficiency in a Loop Bioreactor
This study systematically investigates the influence of liquid level height ([Formula: see text]) on hydrodynamic performance and mixing efficiency in loop bioreactors, a promising technology for energy-efficient single-cell protein production. Through controlled tracer experiments across varied liq...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Chemical Engineering of Japan |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/00219592.2025.2525541 |
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| author | Fan Xu Xiaoping Guan Ning Yang Maximilian Lackner |
| author_facet | Fan Xu Xiaoping Guan Ning Yang Maximilian Lackner |
| author_sort | Fan Xu |
| collection | DOAJ |
| description | This study systematically investigates the influence of liquid level height ([Formula: see text]) on hydrodynamic performance and mixing efficiency in loop bioreactors, a promising technology for energy-efficient single-cell protein production. Through controlled tracer experiments across varied liquid levels ([Formula: see text] to 3) and stirring speeds (1200–1800 rpm), we demonstrate that reduced liquid levels ([Formula: see text]) induce gas entrainment at high speeds ([Formula: see text] 1600 rpm), increasing flow resistance and reducing mean fluid velocity. Conversely, elevated liquid levels ([Formula: see text]) enhance diffusion-driven mixing, shortening mixing time by 2/3 at [Formula: see text] compared to [Formula: see text] A critical transition from convection-dominated to diffusion-dominated regimes is identified at [Formula: see text] with mixing efficiency becoming less sensitive to stirring speed at [Formula: see text] These findings provide feasible guidelines for optimizing loop scalability and advancing reactor optimization strategies for continuous bioprocessing. |
| format | Article |
| id | doaj-art-0d01ecd853fe428ca87e973536b91601 |
| institution | Kabale University |
| issn | 0021-9592 1881-1299 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Chemical Engineering of Japan |
| spelling | doaj-art-0d01ecd853fe428ca87e973536b916012025-08-20T03:50:15ZengTaylor & Francis GroupJournal of Chemical Engineering of Japan0021-95921881-12992025-12-0158110.1080/00219592.2025.2525541Influence of Liquid Level Height on Flow Characteristics and Mixing Efficiency in a Loop BioreactorFan Xu0Xiaoping Guan1Ning Yang2Maximilian Lackner3State Key Laboratory of Mesoscience and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mesoscience and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Mesoscience and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, ChinaCirce Biotechnologie GmbH, Vienna, AustriaThis study systematically investigates the influence of liquid level height ([Formula: see text]) on hydrodynamic performance and mixing efficiency in loop bioreactors, a promising technology for energy-efficient single-cell protein production. Through controlled tracer experiments across varied liquid levels ([Formula: see text] to 3) and stirring speeds (1200–1800 rpm), we demonstrate that reduced liquid levels ([Formula: see text]) induce gas entrainment at high speeds ([Formula: see text] 1600 rpm), increasing flow resistance and reducing mean fluid velocity. Conversely, elevated liquid levels ([Formula: see text]) enhance diffusion-driven mixing, shortening mixing time by 2/3 at [Formula: see text] compared to [Formula: see text] A critical transition from convection-dominated to diffusion-dominated regimes is identified at [Formula: see text] with mixing efficiency becoming less sensitive to stirring speed at [Formula: see text] These findings provide feasible guidelines for optimizing loop scalability and advancing reactor optimization strategies for continuous bioprocessing.https://www.tandfonline.com/doi/10.1080/00219592.2025.2525541Loop bioreactorLiquid level heightGas entrainmentHydrodynamic stabilityMixing efficiency |
| spellingShingle | Fan Xu Xiaoping Guan Ning Yang Maximilian Lackner Influence of Liquid Level Height on Flow Characteristics and Mixing Efficiency in a Loop Bioreactor Journal of Chemical Engineering of Japan Loop bioreactor Liquid level height Gas entrainment Hydrodynamic stability Mixing efficiency |
| title | Influence of Liquid Level Height on Flow Characteristics and Mixing Efficiency in a Loop Bioreactor |
| title_full | Influence of Liquid Level Height on Flow Characteristics and Mixing Efficiency in a Loop Bioreactor |
| title_fullStr | Influence of Liquid Level Height on Flow Characteristics and Mixing Efficiency in a Loop Bioreactor |
| title_full_unstemmed | Influence of Liquid Level Height on Flow Characteristics and Mixing Efficiency in a Loop Bioreactor |
| title_short | Influence of Liquid Level Height on Flow Characteristics and Mixing Efficiency in a Loop Bioreactor |
| title_sort | influence of liquid level height on flow characteristics and mixing efficiency in a loop bioreactor |
| topic | Loop bioreactor Liquid level height Gas entrainment Hydrodynamic stability Mixing efficiency |
| url | https://www.tandfonline.com/doi/10.1080/00219592.2025.2525541 |
| work_keys_str_mv | AT fanxu influenceofliquidlevelheightonflowcharacteristicsandmixingefficiencyinaloopbioreactor AT xiaopingguan influenceofliquidlevelheightonflowcharacteristicsandmixingefficiencyinaloopbioreactor AT ningyang influenceofliquidlevelheightonflowcharacteristicsandmixingefficiencyinaloopbioreactor AT maximilianlackner influenceofliquidlevelheightonflowcharacteristicsandmixingefficiencyinaloopbioreactor |