Indirect Spectrofluorometric Method for the Determination of Cefotaxime Sodium, Ciprofloxacin Hydrochloride and Famotidine in Pharmaceuticals Using Bromate-Bromide and Acriflavine Dye
An Indirect simple sensitive and applicable spectrofluorometric method has been developed for the determination of Cefotaxime Sodium (CEF), ciprofloxacin Hydrochloride (CIP) and Famotidine (FAM) using reaction system bromate-bromide and acriflavine (AF) as fluorescent dye. The method is based on the...
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University of Baghdad, College of Science for Women
2020-03-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4920 |
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| author | Saeed et al. |
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| collection | DOAJ |
| description | An Indirect simple sensitive and applicable spectrofluorometric method has been developed for the determination of Cefotaxime Sodium (CEF), ciprofloxacin Hydrochloride (CIP) and Famotidine (FAM) using reaction system bromate-bromide and acriflavine (AF) as fluorescent dye. The method is based on the oxidation of drugs with known excess bromate-bromide mixture in acidic medium and subsequent determination of unreacted oxidant by quenching fluorescence of AF. Fluorescence intensity of residual AF was measured at 528 nm after excitation at 402 nm. The fluorescence-concentration plots were rectilinear over the ranges 0.1-3.0, 0.05-2.6 and 0.1-3.8 µg ml-1 with lower detection limits of 0.013, 0.018 and 0.021 µg ml-1 and quantitation limits of 0.044, 0.060 and 0.069 µg ml-1 for CEF, CIP and FAM respectively. The common excipients and additives didn’t interfere in their determination. The developed method was successfully applied for determination of the studied drugs in their dosage forms resulted in a good agreement with standard British pharmacopeia method and standard addition procedure. |
| format | Article |
| id | doaj-art-0cf11ac3d2cc4f41908deaab0810fdf7 |
| institution | DOAJ |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2020-03-01 |
| publisher | University of Baghdad, College of Science for Women |
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| series | مجلة بغداد للعلوم |
| spelling | doaj-art-0cf11ac3d2cc4f41908deaab0810fdf72025-08-20T02:54:58ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862020-03-0117110.21123/bsj.2020.17.1.0066Indirect Spectrofluorometric Method for the Determination of Cefotaxime Sodium, Ciprofloxacin Hydrochloride and Famotidine in Pharmaceuticals Using Bromate-Bromide and Acriflavine DyeSaeed et al.An Indirect simple sensitive and applicable spectrofluorometric method has been developed for the determination of Cefotaxime Sodium (CEF), ciprofloxacin Hydrochloride (CIP) and Famotidine (FAM) using reaction system bromate-bromide and acriflavine (AF) as fluorescent dye. The method is based on the oxidation of drugs with known excess bromate-bromide mixture in acidic medium and subsequent determination of unreacted oxidant by quenching fluorescence of AF. Fluorescence intensity of residual AF was measured at 528 nm after excitation at 402 nm. The fluorescence-concentration plots were rectilinear over the ranges 0.1-3.0, 0.05-2.6 and 0.1-3.8 µg ml-1 with lower detection limits of 0.013, 0.018 and 0.021 µg ml-1 and quantitation limits of 0.044, 0.060 and 0.069 µg ml-1 for CEF, CIP and FAM respectively. The common excipients and additives didn’t interfere in their determination. The developed method was successfully applied for determination of the studied drugs in their dosage forms resulted in a good agreement with standard British pharmacopeia method and standard addition procedure.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4920Acriflavine, Cefotaxime Sodium, Ciprofloxacin Hydrochloride, Famotidine, Spectrofluorometric Determination. |
| spellingShingle | Saeed et al. Indirect Spectrofluorometric Method for the Determination of Cefotaxime Sodium, Ciprofloxacin Hydrochloride and Famotidine in Pharmaceuticals Using Bromate-Bromide and Acriflavine Dye مجلة بغداد للعلوم Acriflavine, Cefotaxime Sodium, Ciprofloxacin Hydrochloride, Famotidine, Spectrofluorometric Determination. |
| title | Indirect Spectrofluorometric Method for the Determination of Cefotaxime Sodium, Ciprofloxacin Hydrochloride and Famotidine in Pharmaceuticals Using Bromate-Bromide and Acriflavine Dye |
| title_full | Indirect Spectrofluorometric Method for the Determination of Cefotaxime Sodium, Ciprofloxacin Hydrochloride and Famotidine in Pharmaceuticals Using Bromate-Bromide and Acriflavine Dye |
| title_fullStr | Indirect Spectrofluorometric Method for the Determination of Cefotaxime Sodium, Ciprofloxacin Hydrochloride and Famotidine in Pharmaceuticals Using Bromate-Bromide and Acriflavine Dye |
| title_full_unstemmed | Indirect Spectrofluorometric Method for the Determination of Cefotaxime Sodium, Ciprofloxacin Hydrochloride and Famotidine in Pharmaceuticals Using Bromate-Bromide and Acriflavine Dye |
| title_short | Indirect Spectrofluorometric Method for the Determination of Cefotaxime Sodium, Ciprofloxacin Hydrochloride and Famotidine in Pharmaceuticals Using Bromate-Bromide and Acriflavine Dye |
| title_sort | indirect spectrofluorometric method for the determination of cefotaxime sodium ciprofloxacin hydrochloride and famotidine in pharmaceuticals using bromate bromide and acriflavine dye |
| topic | Acriflavine, Cefotaxime Sodium, Ciprofloxacin Hydrochloride, Famotidine, Spectrofluorometric Determination. |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4920 |
| work_keys_str_mv | AT saeedetal indirectspectrofluorometricmethodforthedeterminationofcefotaximesodiumciprofloxacinhydrochlorideandfamotidineinpharmaceuticalsusingbromatebromideandacriflavinedye |