Establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple device
Intracellular potassium ion (K+) concentration is higher than extracellular K+ concentration. Some cells maintain intracellular potassium levels by taking up extracellular potassium. However, investigating these details requires techniques to measure intracellular potassium concentrations. We establ...
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AIMS Press
2024-08-01
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| Series: | AIMS Neuroscience |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/Neuroscience.2024018?viewType=HTML |
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| author | Takaya Iwamoto Minori Fujita Yukiko Futamata Teruki Okada Ryuta Morinaga Airi Nishi Toshihiko Kinjo Koichi Kawada Kyosuke Uno Nobuyuki Kuramoto |
| author_facet | Takaya Iwamoto Minori Fujita Yukiko Futamata Teruki Okada Ryuta Morinaga Airi Nishi Toshihiko Kinjo Koichi Kawada Kyosuke Uno Nobuyuki Kuramoto |
| author_sort | Takaya Iwamoto |
| collection | DOAJ |
| description | Intracellular potassium ion (K+) concentration is higher than extracellular K+ concentration. Some cells maintain intracellular potassium levels by taking up extracellular potassium. However, investigating these details requires techniques to measure intracellular potassium concentrations. We established a method to easily measure intracellular potassium concentrations using a simpler electrode. The LAQUAtwin K-11 was capable of linearly quantifying potassium concentrations and was not affected by cellular constituents other than nucleic acids; however, it did not tolerate low temperatures. Interference caused by a high concentration of nucleic acids was eliminated by the addition of cations. It was also suggested that the concentration of nucleic acids in the cell suspension was not sufficiently high to interfere with the measurements. Intracellular potassium concentrations increased and decreased in response to extracellular potassium concentrations. Exposure to valinomycin did not decrease the potassium concentration, suggesting that re-uptake of the potassium released outside the cells occurred immediately. Additionally, potassium concentrations could be measured in the brain tissue homogenates using the device. This measurement method can track the relative changes in potassium concentration in cells under various conditions and in tissues of various disease models. |
| format | Article |
| id | doaj-art-0cf0b82f9d1649e2a83a292b052d96bd |
| institution | OA Journals |
| issn | 2373-7972 |
| language | English |
| publishDate | 2024-08-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Neuroscience |
| spelling | doaj-art-0cf0b82f9d1649e2a83a292b052d96bd2025-08-20T01:47:36ZengAIMS PressAIMS Neuroscience2373-79722024-08-0111327528810.3934/Neuroscience.2024018Establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple deviceTakaya Iwamoto0Minori Fujita 1Yukiko Futamata2Teruki Okada3Ryuta Morinaga4Airi Nishi 5Toshihiko Kinjo 6Koichi Kawada 7 Kyosuke Uno 8Nobuyuki Kuramoto 9Laboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanLaboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan University, Osaka, JapanIntracellular potassium ion (K+) concentration is higher than extracellular K+ concentration. Some cells maintain intracellular potassium levels by taking up extracellular potassium. However, investigating these details requires techniques to measure intracellular potassium concentrations. We established a method to easily measure intracellular potassium concentrations using a simpler electrode. The LAQUAtwin K-11 was capable of linearly quantifying potassium concentrations and was not affected by cellular constituents other than nucleic acids; however, it did not tolerate low temperatures. Interference caused by a high concentration of nucleic acids was eliminated by the addition of cations. It was also suggested that the concentration of nucleic acids in the cell suspension was not sufficiently high to interfere with the measurements. Intracellular potassium concentrations increased and decreased in response to extracellular potassium concentrations. Exposure to valinomycin did not decrease the potassium concentration, suggesting that re-uptake of the potassium released outside the cells occurred immediately. Additionally, potassium concentrations could be measured in the brain tissue homogenates using the device. This measurement method can track the relative changes in potassium concentration in cells under various conditions and in tissues of various disease models.https://www.aimspress.com/article/doi/10.3934/Neuroscience.2024018?viewType=HTMLpotassium ionion metersimple devicehomeostasis of potassium ionintracellular concentration of potassium ioncentral nervous system tissue |
| spellingShingle | Takaya Iwamoto Minori Fujita Yukiko Futamata Teruki Okada Ryuta Morinaga Airi Nishi Toshihiko Kinjo Koichi Kawada Kyosuke Uno Nobuyuki Kuramoto Establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple device AIMS Neuroscience potassium ion ion meter simple device homeostasis of potassium ion intracellular concentration of potassium ion central nervous system tissue |
| title | Establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple device |
| title_full | Establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple device |
| title_fullStr | Establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple device |
| title_full_unstemmed | Establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple device |
| title_short | Establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple device |
| title_sort | establishment of a method to measure the intracellular potassium ion concentration of brain tissue using a simple device |
| topic | potassium ion ion meter simple device homeostasis of potassium ion intracellular concentration of potassium ion central nervous system tissue |
| url | https://www.aimspress.com/article/doi/10.3934/Neuroscience.2024018?viewType=HTML |
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