Trimetazidine effects on intracellular Са2+ ion concentration in HL-60 human promyelocytes
Aim. To investigate the effects of an anti-ischemic medication trimetazidine on Са2+ ion metabolism in human leukocyte and monocyte precursors – HL-60 promyelocytes.Material and methods. HL-60 cells were cultivated in vitro. Changes in intracellular free Са2+ ion concentration were registered with f...
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| Format: | Article |
| Language: | Russian |
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«SILICEA-POLIGRAF» LLC
2008-10-01
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| Series: | Кардиоваскулярная терапия и профилактика |
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| Online Access: | https://cardiovascular.elpub.ru/jour/article/view/1102 |
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| author | E. I. Astashkin M. G. Glezer S. V. Grachev |
| author_facet | E. I. Astashkin M. G. Glezer S. V. Grachev |
| author_sort | E. I. Astashkin |
| collection | DOAJ |
| description | Aim. To investigate the effects of an anti-ischemic medication trimetazidine on Са2+ ion metabolism in human leukocyte and monocyte precursors – HL-60 promyelocytes.Material and methods. HL-60 cells were cultivated in vitro. Changes in intracellular free Са2+ ion concentration were registered with fluorescent Са2+ probes Fura-2. Results. For the first time it was demonstrated for differentiated and non-differentiated HL-60 cells that an antianginal agent trimetazidine suppressed the activity of plasmatic membrane store operated Са2+ channels (SOC channels). Importantly, trimetazidine did not cause Са2+ ion release from the stores and did not open SOC channels, in contrast to other SOC channel inhibitors, e.g., miconazole. In open SOC channels, trimetazidine and miconazole targeted the same binding areas.Conclusion. The results obtained demonstrate additional trimetazidine effects, linked to selective SOC channel blockade. It provides new potential explanations for trimetazidine effects on electro-inert cells (e.g. blood phagocytes) and its effects in myocardial ischemia. |
| format | Article |
| id | doaj-art-0cef02cebf0c4f449d7de0b8bded009f |
| institution | DOAJ |
| issn | 1728-8800 2619-0125 |
| language | Russian |
| publishDate | 2008-10-01 |
| publisher | «SILICEA-POLIGRAF» LLC |
| record_format | Article |
| series | Кардиоваскулярная терапия и профилактика |
| spelling | doaj-art-0cef02cebf0c4f449d7de0b8bded009f2025-08-20T02:59:54Zrus«SILICEA-POLIGRAF» LLCКардиоваскулярная терапия и профилактика1728-88002619-01252008-10-01756267814Trimetazidine effects on intracellular Са2+ ion concentration in HL-60 human promyelocytesE. I. Astashkin0M. G. Glezer1S. V. Grachev2I.M. Sechenov Moscow Medical Academy. MoscowI.M. Sechenov Moscow Medical Academy. MoscowI.M. Sechenov Moscow Medical Academy. MoscowAim. To investigate the effects of an anti-ischemic medication trimetazidine on Са2+ ion metabolism in human leukocyte and monocyte precursors – HL-60 promyelocytes.Material and methods. HL-60 cells were cultivated in vitro. Changes in intracellular free Са2+ ion concentration were registered with fluorescent Са2+ probes Fura-2. Results. For the first time it was demonstrated for differentiated and non-differentiated HL-60 cells that an antianginal agent trimetazidine suppressed the activity of plasmatic membrane store operated Са2+ channels (SOC channels). Importantly, trimetazidine did not cause Са2+ ion release from the stores and did not open SOC channels, in contrast to other SOC channel inhibitors, e.g., miconazole. In open SOC channels, trimetazidine and miconazole targeted the same binding areas.Conclusion. The results obtained demonstrate additional trimetazidine effects, linked to selective SOC channel blockade. It provides new potential explanations for trimetazidine effects on electro-inert cells (e.g. blood phagocytes) and its effects in myocardial ischemia.https://cardiovascular.elpub.ru/jour/article/view/1102trimetazidinehl-60 cellssoc channelsregulation of intracellular free са2+ ion concentration |
| spellingShingle | E. I. Astashkin M. G. Glezer S. V. Grachev Trimetazidine effects on intracellular Са2+ ion concentration in HL-60 human promyelocytes Кардиоваскулярная терапия и профилактика trimetazidine hl-60 cells soc channels regulation of intracellular free са2+ ion concentration |
| title | Trimetazidine effects on intracellular Са2+ ion concentration in HL-60 human promyelocytes |
| title_full | Trimetazidine effects on intracellular Са2+ ion concentration in HL-60 human promyelocytes |
| title_fullStr | Trimetazidine effects on intracellular Са2+ ion concentration in HL-60 human promyelocytes |
| title_full_unstemmed | Trimetazidine effects on intracellular Са2+ ion concentration in HL-60 human promyelocytes |
| title_short | Trimetazidine effects on intracellular Са2+ ion concentration in HL-60 human promyelocytes |
| title_sort | trimetazidine effects on intracellular са2 ion concentration in hl 60 human promyelocytes |
| topic | trimetazidine hl-60 cells soc channels regulation of intracellular free са2+ ion concentration |
| url | https://cardiovascular.elpub.ru/jour/article/view/1102 |
| work_keys_str_mv | AT eiastashkin trimetazidineeffectsonintracellularsa2ionconcentrationinhl60humanpromyelocytes AT mgglezer trimetazidineeffectsonintracellularsa2ionconcentrationinhl60humanpromyelocytes AT svgrachev trimetazidineeffectsonintracellularsa2ionconcentrationinhl60humanpromyelocytes |