Identification and characterization of fungi associated with leaf spot/blight and melting-out of turfgrass in Algeria
Symptoms of foliar blight were observed on turfgrass in Oran (Algeria), including yellow chlorotic patches on leaves during the 2020 summer (temperatures between 35 and 40°C). Symptoms extended downward from leaf tips and entire leaves became blighted, leading to irregular discoloured areas that lat...
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Firenze University Press
2023-05-01
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| Series: | Phytopathologia Mediterranea |
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| Online Access: | https://oajournals.fupress.net/index.php/pm/article/view/14169 |
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| author | Nabahat BESSADAT Bruno HAMON Nelly BATAILLÉ-SIMONEAU Nisserine HAMINI-KADAR Mabrouk KIHAL Philippe SIMONEAU |
| author_facet | Nabahat BESSADAT Bruno HAMON Nelly BATAILLÉ-SIMONEAU Nisserine HAMINI-KADAR Mabrouk KIHAL Philippe SIMONEAU |
| author_sort | Nabahat BESSADAT |
| collection | DOAJ |
| description | Symptoms of foliar blight were observed on turfgrass in Oran (Algeria), including yellow chlorotic patches on leaves during the 2020 summer (temperatures between 35 and 40°C). Symptoms extended downward from leaf tips and entire leaves became blighted, leading to irregular discoloured areas that later turned brown. Isolations from infected plants included 214 isolates identified as Curvularia or Bipolaris, based on morphological traits. Other isolates included Fusarium, Myrothecium and Acremonium spp. Three molecular loci, ITS rDNA, gpd and tef1, were amplified and sequenced. Morphological and multi-locus phylogenetic analyses revealed four fungal species viz. B. sorokiniana, C. spicifera, C. verruculosa, C. geniculata, and two additional Curvularia lineages, some of these fungi are reported are first records for Algeria. Koch’s postulates were confirmed by inoculating potted turfgrass with spore suspensions of 16 isolates and re-isolating of the inoculated pathogens from symptomatic tissues. Bipolaris sorokiniana was the most virulent pathogen causing numerous foliar necrotic lesions similar to those observed in the field. Other isolates infected basal leaves only, and caused less severe symptoms. The results show that Curvularia species may be secondary pathogens infecting stressed plants, and that simultaneous occurrence of high temperatures and poor water quality have influenced disease progression. Correct identification of these pathogens is important for applying appropriate and timely disease management. |
| format | Article |
| id | doaj-art-0ce8b258cfbb41b590f1e54402acb251 |
| institution | DOAJ |
| issn | 0031-9465 1593-2095 |
| language | English |
| publishDate | 2023-05-01 |
| publisher | Firenze University Press |
| record_format | Article |
| series | Phytopathologia Mediterranea |
| spelling | doaj-art-0ce8b258cfbb41b590f1e54402acb2512025-08-20T03:05:25ZengFirenze University PressPhytopathologia Mediterranea0031-94651593-20952023-05-01621739310.36253/phyto-1416913012Identification and characterization of fungi associated with leaf spot/blight and melting-out of turfgrass in AlgeriaNabahat BESSADAT0https://orcid.org/0000-0001-7795-2606Bruno HAMON1https://orcid.org/0000-0001-5868-0676Nelly BATAILLÉ-SIMONEAU2https://orcid.org/0000-0001-6200-4259Nisserine HAMINI-KADAR3https://orcid.org/0000-0002-3240-5913Mabrouk KIHAL4https://orcid.org/0000-0003-2901-373XPhilippe SIMONEAU5https://orcid.org/0000-0002-3890-9848Université Angers, Institut Agro, INRAE, UMR 1345 IRHS, SFR QUASAV, Beaucouzé, 49071Université Angers, Institut Agro, INRAE, UMR 1345 IRHS, SFR QUASAV, Beaucouzé, 49071Université Angers, Institut Agro, INRAE, UMR 1345 IRHS, SFR QUASAV, Beaucouzé, 49071Université Oran1 Ahmed Ben Bella, Laboratoire de Microbiologie Appliquée, BP1524 El M’naouer 31000 OranUniversité Oran1 Ahmed Ben Bella, Laboratoire de Microbiologie Appliquée, BP1524 El M’naouer 31000 OranUniversité Angers, Institut Agro, INRAE, UMR 1345 IRHS, SFR QUASAV, Beaucouzé, 49071Symptoms of foliar blight were observed on turfgrass in Oran (Algeria), including yellow chlorotic patches on leaves during the 2020 summer (temperatures between 35 and 40°C). Symptoms extended downward from leaf tips and entire leaves became blighted, leading to irregular discoloured areas that later turned brown. Isolations from infected plants included 214 isolates identified as Curvularia or Bipolaris, based on morphological traits. Other isolates included Fusarium, Myrothecium and Acremonium spp. Three molecular loci, ITS rDNA, gpd and tef1, were amplified and sequenced. Morphological and multi-locus phylogenetic analyses revealed four fungal species viz. B. sorokiniana, C. spicifera, C. verruculosa, C. geniculata, and two additional Curvularia lineages, some of these fungi are reported are first records for Algeria. Koch’s postulates were confirmed by inoculating potted turfgrass with spore suspensions of 16 isolates and re-isolating of the inoculated pathogens from symptomatic tissues. Bipolaris sorokiniana was the most virulent pathogen causing numerous foliar necrotic lesions similar to those observed in the field. Other isolates infected basal leaves only, and caused less severe symptoms. The results show that Curvularia species may be secondary pathogens infecting stressed plants, and that simultaneous occurrence of high temperatures and poor water quality have influenced disease progression. Correct identification of these pathogens is important for applying appropriate and timely disease management.https://oajournals.fupress.net/index.php/pm/article/view/14169morphological characterizationmulti-gene phylogenykoch’s postulatesbipolaris spp.a. carbonarius |
| spellingShingle | Nabahat BESSADAT Bruno HAMON Nelly BATAILLÉ-SIMONEAU Nisserine HAMINI-KADAR Mabrouk KIHAL Philippe SIMONEAU Identification and characterization of fungi associated with leaf spot/blight and melting-out of turfgrass in Algeria Phytopathologia Mediterranea morphological characterizationmulti-gene phylogeny koch’s postulates bipolaris spp. a. carbonarius |
| title | Identification and characterization of fungi associated with leaf spot/blight and melting-out of turfgrass in Algeria |
| title_full | Identification and characterization of fungi associated with leaf spot/blight and melting-out of turfgrass in Algeria |
| title_fullStr | Identification and characterization of fungi associated with leaf spot/blight and melting-out of turfgrass in Algeria |
| title_full_unstemmed | Identification and characterization of fungi associated with leaf spot/blight and melting-out of turfgrass in Algeria |
| title_short | Identification and characterization of fungi associated with leaf spot/blight and melting-out of turfgrass in Algeria |
| title_sort | identification and characterization of fungi associated with leaf spot blight and melting out of turfgrass in algeria |
| topic | morphological characterizationmulti-gene phylogeny koch’s postulates bipolaris spp. a. carbonarius |
| url | https://oajournals.fupress.net/index.php/pm/article/view/14169 |
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