The fifth adaptor protein complex.
Adaptor protein (AP) complexes sort cargo into vesicles for transport from one membrane compartment of the cell to another. Four distinct AP complexes have been identified, which are present in most eukaryotes. We report the existence of a fifth AP complex, AP-5. Tagged AP-5 localises to a late endo...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-10-01
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| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.1001170&type=printable |
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| author | Jennifer Hirst Lael D Barlow Gabriel Casey Francisco Daniela A Sahlender Matthew N J Seaman Joel B Dacks Margaret S Robinson |
| author_facet | Jennifer Hirst Lael D Barlow Gabriel Casey Francisco Daniela A Sahlender Matthew N J Seaman Joel B Dacks Margaret S Robinson |
| author_sort | Jennifer Hirst |
| collection | DOAJ |
| description | Adaptor protein (AP) complexes sort cargo into vesicles for transport from one membrane compartment of the cell to another. Four distinct AP complexes have been identified, which are present in most eukaryotes. We report the existence of a fifth AP complex, AP-5. Tagged AP-5 localises to a late endosomal compartment in HeLa cells. AP-5 does not associate with clathrin and is insensitive to brefeldin A. Knocking down AP-5 subunits interferes with the trafficking of the cation-independent mannose 6-phosphate receptor and causes the cell to form swollen endosomal structures with emanating tubules. AP-5 subunits can be found in all five eukaryotic supergroups, but they have been co-ordinately lost in many organisms. Concatenated phylogenetic analysis provides robust resolution, for the first time, into the evolutionary order of emergence of the adaptor subunit families, showing AP-3 as the basal complex, followed by AP-5, AP-4, and AP-1 and AP-2. Thus, AP-5 is an evolutionarily ancient complex, which is involved in endosomal sorting, and which has links with hereditary spastic paraplegia. |
| format | Article |
| id | doaj-art-0ce8ae3c5c374d01acf2edd3c4202f5c |
| institution | OA Journals |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2011-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj-art-0ce8ae3c5c374d01acf2edd3c4202f5c2025-08-20T02:34:06ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852011-10-01910e100117010.1371/journal.pbio.1001170The fifth adaptor protein complex.Jennifer HirstLael D BarlowGabriel Casey FranciscoDaniela A SahlenderMatthew N J SeamanJoel B DacksMargaret S RobinsonAdaptor protein (AP) complexes sort cargo into vesicles for transport from one membrane compartment of the cell to another. Four distinct AP complexes have been identified, which are present in most eukaryotes. We report the existence of a fifth AP complex, AP-5. Tagged AP-5 localises to a late endosomal compartment in HeLa cells. AP-5 does not associate with clathrin and is insensitive to brefeldin A. Knocking down AP-5 subunits interferes with the trafficking of the cation-independent mannose 6-phosphate receptor and causes the cell to form swollen endosomal structures with emanating tubules. AP-5 subunits can be found in all five eukaryotic supergroups, but they have been co-ordinately lost in many organisms. Concatenated phylogenetic analysis provides robust resolution, for the first time, into the evolutionary order of emergence of the adaptor subunit families, showing AP-3 as the basal complex, followed by AP-5, AP-4, and AP-1 and AP-2. Thus, AP-5 is an evolutionarily ancient complex, which is involved in endosomal sorting, and which has links with hereditary spastic paraplegia.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.1001170&type=printable |
| spellingShingle | Jennifer Hirst Lael D Barlow Gabriel Casey Francisco Daniela A Sahlender Matthew N J Seaman Joel B Dacks Margaret S Robinson The fifth adaptor protein complex. PLoS Biology |
| title | The fifth adaptor protein complex. |
| title_full | The fifth adaptor protein complex. |
| title_fullStr | The fifth adaptor protein complex. |
| title_full_unstemmed | The fifth adaptor protein complex. |
| title_short | The fifth adaptor protein complex. |
| title_sort | fifth adaptor protein complex |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.1001170&type=printable |
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