Mechanism of Swimming-Induced Inhibition of Colorectal Cancer Progression via the Wnt/β-catenin Signaling Pathway
ObjectiveTo explore the mechanism by which swimming inhibits the progression of colorectal cancer (CRC) in mice through the Wnt/β-catenin signaling pathway.Methods<italic>In vitro</italic> experiments were conducted where CT26 cells in good growth condition were randomly divided into neg...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Office of Rehabilitation Medicine
2025-02-01
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| Series: | 康复学报 |
| Subjects: | |
| Online Access: | http://kfxb.publish.founderss.cn/thesisDetails#10.3724/SP.J.1329.2025.01007 |
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| Summary: | ObjectiveTo explore the mechanism by which swimming inhibits the progression of colorectal cancer (CRC) in mice through the Wnt/β-catenin signaling pathway.Methods<italic>In vitro</italic> experiments were conducted where CT26 cells in good growth condition were randomly divided into negative control group (si NC group) and β-catenin knockdown group (si β-catenin group). After knockdown of the β-catenin gene by siRNA technology, the proliferation of CT26 cells was observed under a microscope, and the cells were counted. Western blot method was used to detect the expression levels of downstream related factors β-catenin, c-Myc, Cyclin D1, and Axin2 in the Wnt/β-catenin signaling pathway. <italic>In vivo</italic> experiments, sixteen healthy male 8-week-old BALB/C mice were randomly divided into control group and swimming group with 8 mice in each group. The control group was placed in a dry container, while the swimming group underwent swimming training in a pool, six times a week, 30 minutes each time, for a total of 3 weeks. The mice were then sacrificed and their body weight was measured. The levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatinine (Cr), and blood urea nitrogen (BUN) in the serum were detected using corresponding assay kits; Tumor volumes were determined by electronic vernier calipers, and the tumor mass was weighed and photographed. Immunohistochemistry (IHC) was used to detect the expression of proliferating cell nuclear antigen Ki-67 in tumor tissues. Western blot was used to detect the relative expression levels of total protein phospho-β-catenin and nuclear β-catenin in tumor tissues.ResultsCompared with the si NC group, the cell number in the si β-catenin group was significantly reduced (<italic>P</italic><0.05); Compared with the si NC group, the protein expression levels of β-catenin, c-Myc, Cyclin D1, and Axin2 in the si β-catenin group significantly decreased (<italic>P</italic><0.05). Compared with the control group, the swimming group showed no statistically significant difference in body weight (<italic>P</italic>>0.05). Compared with the control group, the swimming group showed no statistically significant difference in the levels of ALT, AST, Cr and BUN in the serum (<italic>P</italic>>0.05). Compared with the control group, the tumor volume was significantly reduced (<italic>P</italic><0.05), and tumor mass decreased in the swimming group (<italic>P</italic><0.05). Compared with the control group, the expression of Ki-67 in the tumor tissues of the swimming group significantly decreased (<italic>P</italic><0.05); compared with the control group, the total protein level of p-β- catenin significantly increased (P<0.05), and the expression level of nuclear β-catenin in the swimming group decreased significantly (<italic>P</italic><0.05).ConclusionThis study indicates that swimming can inhibit the progression of colorectal tumors in mice by downregulating the Wnt/β-catenin signaling pathway, suggesting that swimming may serve as a potential adjuvant therapy for the recovery of CRC patients mediated by the nuclear translocation of β-catenin. |
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| ISSN: | 2096-0328 |