Diagnosis of polycystic ovaries syndrome: establishment of reference values of nine hormones on Maglumi X- 8
Abstract Background Polycystic ovary syndrome (PCOS) is one of the most common causes of infertility. Availability of some hormones like free testosterone (fT), dehydroepiandrosterone sulfate (DHEAS), anti-Müllerian hormone (AMH), and androstenedione (A4) assay on a user-friendly immunoassay system...
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SpringerOpen
2025-04-01
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| Series: | Middle East Fertility Society Journal |
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| Online Access: | https://doi.org/10.1186/s43043-025-00225-w |
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| author | Aamir Ijaz Shakila Khadim Insharah Ali Sahi Ayesha Akbar Sara Saeed Farhan Ahmed Khalid Muhammad Yasir Waheed |
| author_facet | Aamir Ijaz Shakila Khadim Insharah Ali Sahi Ayesha Akbar Sara Saeed Farhan Ahmed Khalid Muhammad Yasir Waheed |
| author_sort | Aamir Ijaz |
| collection | DOAJ |
| description | Abstract Background Polycystic ovary syndrome (PCOS) is one of the most common causes of infertility. Availability of some hormones like free testosterone (fT), dehydroepiandrosterone sulfate (DHEAS), anti-Müllerian hormone (AMH), and androstenedione (A4) assay on a user-friendly immunoassay system will be a milestone in the diagnosis of PCOS. Aim of the study was to establish reference values of fT, total testosterone (TT), luteinizing hormones (LH), follicle stimulating hormones (FSH), prolactin (Prl), estradiol (E2), DHEAS, AMH, and A4. Results Serum specimens of 135 disease-free adult females were collected from Mohi Uddin Teaching Hospital, Mirpur, AJ&K, Pakistan (MOTH) which is the affiliated hospital of Mohi Uddin Islamic Medical College, Mirpur, AJK, Pakistan (MIMC) and after obtaining written informed consent. Blood specimens were analyzed in the Pathology Laboratory of MOTH using state-of-the-art fully automatic chemiluminescence analyzer Maglumi X8 (Snibe, China) for all nine parameters. Reference values of fertility hormones were AMH: 0.84–10.2 ng/mL (14.3–49.0 pmol/L); A4: 10.5–50.9 ng/dL (3.7–17.8 mmol/L); DHEAS: 81–426 µg/dL (0.8–10.2 µmol/L); E2: 18.9–147 pg/mL (70–542 pmol/L); fT: 0.5–2.57 pg/mL (1.7–8.9 pmol/L); FSH (follicular phase): 2.36–13.1 mIU/mL; LH (follicular phase): 0.66–14.7 mIU/mL; Prl: 3.85–30 ng/mL (81–623 mIU/L); and TT: 0.98–8.2 ng/dL (0.03–0.28 nmol/L). Conclusion We have established reference values of nine hormones used for the diagnosis and monitoring of PCOS in Pakistani and other populations of sub-continent. |
| format | Article |
| id | doaj-art-0ce58c341f9c4aa696c0573804be8cc2 |
| institution | DOAJ |
| issn | 2090-3251 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Middle East Fertility Society Journal |
| spelling | doaj-art-0ce58c341f9c4aa696c0573804be8cc22025-08-20T03:06:48ZengSpringerOpenMiddle East Fertility Society Journal2090-32512025-04-013011910.1186/s43043-025-00225-wDiagnosis of polycystic ovaries syndrome: establishment of reference values of nine hormones on Maglumi X- 8Aamir Ijaz0Shakila Khadim1Insharah Ali Sahi2Ayesha Akbar3Sara Saeed4Farhan Ahmed5Khalid Muhammad6Yasir Waheed7NUST School of Health Sciences (NSHS), National University of Sciences and Technology (NUST)Mohi Uddin Islamic Medical College and HospitalMohi Uddin Islamic Medical College and HospitalMohi Uddin Islamic Medical College and HospitalMohi Uddin Islamic Medical College and HospitalMilton Keynes University Hospital NHS Trust, Oxford University HospitalsDepartment of Biology, College of Science, UAE UniversityNUST School of Health Sciences (NSHS), National University of Sciences and Technology (NUST)Abstract Background Polycystic ovary syndrome (PCOS) is one of the most common causes of infertility. Availability of some hormones like free testosterone (fT), dehydroepiandrosterone sulfate (DHEAS), anti-Müllerian hormone (AMH), and androstenedione (A4) assay on a user-friendly immunoassay system will be a milestone in the diagnosis of PCOS. Aim of the study was to establish reference values of fT, total testosterone (TT), luteinizing hormones (LH), follicle stimulating hormones (FSH), prolactin (Prl), estradiol (E2), DHEAS, AMH, and A4. Results Serum specimens of 135 disease-free adult females were collected from Mohi Uddin Teaching Hospital, Mirpur, AJ&K, Pakistan (MOTH) which is the affiliated hospital of Mohi Uddin Islamic Medical College, Mirpur, AJK, Pakistan (MIMC) and after obtaining written informed consent. Blood specimens were analyzed in the Pathology Laboratory of MOTH using state-of-the-art fully automatic chemiluminescence analyzer Maglumi X8 (Snibe, China) for all nine parameters. Reference values of fertility hormones were AMH: 0.84–10.2 ng/mL (14.3–49.0 pmol/L); A4: 10.5–50.9 ng/dL (3.7–17.8 mmol/L); DHEAS: 81–426 µg/dL (0.8–10.2 µmol/L); E2: 18.9–147 pg/mL (70–542 pmol/L); fT: 0.5–2.57 pg/mL (1.7–8.9 pmol/L); FSH (follicular phase): 2.36–13.1 mIU/mL; LH (follicular phase): 0.66–14.7 mIU/mL; Prl: 3.85–30 ng/mL (81–623 mIU/L); and TT: 0.98–8.2 ng/dL (0.03–0.28 nmol/L). Conclusion We have established reference values of nine hormones used for the diagnosis and monitoring of PCOS in Pakistani and other populations of sub-continent.https://doi.org/10.1186/s43043-025-00225-wLuteinizing hormones (LH)Follicle stimulating hormones (FSH)ProlactinEstradiolTotal testosteroneFree testosterone |
| spellingShingle | Aamir Ijaz Shakila Khadim Insharah Ali Sahi Ayesha Akbar Sara Saeed Farhan Ahmed Khalid Muhammad Yasir Waheed Diagnosis of polycystic ovaries syndrome: establishment of reference values of nine hormones on Maglumi X- 8 Middle East Fertility Society Journal Luteinizing hormones (LH) Follicle stimulating hormones (FSH) Prolactin Estradiol Total testosterone Free testosterone |
| title | Diagnosis of polycystic ovaries syndrome: establishment of reference values of nine hormones on Maglumi X- 8 |
| title_full | Diagnosis of polycystic ovaries syndrome: establishment of reference values of nine hormones on Maglumi X- 8 |
| title_fullStr | Diagnosis of polycystic ovaries syndrome: establishment of reference values of nine hormones on Maglumi X- 8 |
| title_full_unstemmed | Diagnosis of polycystic ovaries syndrome: establishment of reference values of nine hormones on Maglumi X- 8 |
| title_short | Diagnosis of polycystic ovaries syndrome: establishment of reference values of nine hormones on Maglumi X- 8 |
| title_sort | diagnosis of polycystic ovaries syndrome establishment of reference values of nine hormones on maglumi x 8 |
| topic | Luteinizing hormones (LH) Follicle stimulating hormones (FSH) Prolactin Estradiol Total testosterone Free testosterone |
| url | https://doi.org/10.1186/s43043-025-00225-w |
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