Purinergic inhibitory regulation of esophageal smooth muscle is mediated by P2Y receptors and ATP-dependent potassium channels in rats
Abstract Purines such as ATP are regulatory transmitters in motility of the gastrointestinal tract. The aims of this study were to propose functional roles of purinergic regulation of esophageal motility. An isolated segment of the rat esophagus was placed in an organ bath, and mechanical responses...
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Elsevier
2024-04-01
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| Series: | Journal of Physiological Sciences |
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| Online Access: | https://doi.org/10.1186/s12576-024-00916-5 |
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| author | Takahiko Shiina Yuji Suzuki Kazuhiro Horii Tomoya Sawamura Natsufu Yuki Yuuki Horii Yasutake Shimizu |
| author_facet | Takahiko Shiina Yuji Suzuki Kazuhiro Horii Tomoya Sawamura Natsufu Yuki Yuuki Horii Yasutake Shimizu |
| author_sort | Takahiko Shiina |
| collection | DOAJ |
| description | Abstract Purines such as ATP are regulatory transmitters in motility of the gastrointestinal tract. The aims of this study were to propose functional roles of purinergic regulation of esophageal motility. An isolated segment of the rat esophagus was placed in an organ bath, and mechanical responses were recorded using a force transducer. Exogenous application of ATP (10–100 μM) evoked relaxation of the esophageal smooth muscle in a longitudinal direction under the condition of carbachol (1 μM) -induced precontraction. Pretreatment with a non-selective P2 receptor antagonist, suramin (500 μM), and a P2Y receptor antagonist, cibacron blue F3GA (200 μM), inhibited the ATP (100 μM) -induced relaxation, but a P2X receptor antagonist, pyridoxal phosphate-6-azophenyl-2,4-disulfonic acid (50 μM), did not affect it. A blocker of ATP-dependent potassium channels (KATP channels), glibenclamide (200 μM), inhibited the ATP-induced relaxation and application of an opener of KATP channels, nicorandil (50 μM), produced relaxation. The findings suggest that ATP is involved in inhibitory regulation of the longitudinal smooth muscle in the muscularis mucosae of the rat esophagus via activation of P2Y receptors and then opening of KATP channels. |
| format | Article |
| id | doaj-art-0ce033dac2624bf8b2bae08339892a12 |
| institution | Kabale University |
| issn | 1880-6562 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
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| series | Journal of Physiological Sciences |
| spelling | doaj-art-0ce033dac2624bf8b2bae08339892a122025-08-20T03:54:15ZengElsevierJournal of Physiological Sciences1880-65622024-04-0174111210.1186/s12576-024-00916-5Purinergic inhibitory regulation of esophageal smooth muscle is mediated by P2Y receptors and ATP-dependent potassium channels in ratsTakahiko Shiina0Yuji Suzuki1Kazuhiro Horii2Tomoya Sawamura3Natsufu Yuki4Yuuki Horii5Yasutake Shimizu6Department of Basic Veterinary Science, Laboratory of Physiology, Joint Graduate School of Veterinary Sciences, Gifu UniversityDepartment of Basic Veterinary Science, Laboratory of Physiology, Joint Graduate School of Veterinary Sciences, Gifu UniversityDivision of Biological Principles, Department of Physiology, Graduate School of Medicine, Gifu UniversityDepartment of Basic Veterinary Science, Laboratory of Physiology, Joint Graduate School of Veterinary Sciences, Gifu UniversityDepartment of Basic Veterinary Science, Laboratory of Physiology, Joint Graduate School of Veterinary Sciences, Gifu UniversityInstitute for Glyco-Core Research (iGCORE), Gifu UniversityDepartment of Basic Veterinary Science, Laboratory of Physiology, Joint Graduate School of Veterinary Sciences, Gifu UniversityAbstract Purines such as ATP are regulatory transmitters in motility of the gastrointestinal tract. The aims of this study were to propose functional roles of purinergic regulation of esophageal motility. An isolated segment of the rat esophagus was placed in an organ bath, and mechanical responses were recorded using a force transducer. Exogenous application of ATP (10–100 μM) evoked relaxation of the esophageal smooth muscle in a longitudinal direction under the condition of carbachol (1 μM) -induced precontraction. Pretreatment with a non-selective P2 receptor antagonist, suramin (500 μM), and a P2Y receptor antagonist, cibacron blue F3GA (200 μM), inhibited the ATP (100 μM) -induced relaxation, but a P2X receptor antagonist, pyridoxal phosphate-6-azophenyl-2,4-disulfonic acid (50 μM), did not affect it. A blocker of ATP-dependent potassium channels (KATP channels), glibenclamide (200 μM), inhibited the ATP-induced relaxation and application of an opener of KATP channels, nicorandil (50 μM), produced relaxation. The findings suggest that ATP is involved in inhibitory regulation of the longitudinal smooth muscle in the muscularis mucosae of the rat esophagus via activation of P2Y receptors and then opening of KATP channels.https://doi.org/10.1186/s12576-024-00916-5ATP-dependent potassium channelEsophagusP2 receptorPurineSmooth muscle |
| spellingShingle | Takahiko Shiina Yuji Suzuki Kazuhiro Horii Tomoya Sawamura Natsufu Yuki Yuuki Horii Yasutake Shimizu Purinergic inhibitory regulation of esophageal smooth muscle is mediated by P2Y receptors and ATP-dependent potassium channels in rats Journal of Physiological Sciences ATP-dependent potassium channel Esophagus P2 receptor Purine Smooth muscle |
| title | Purinergic inhibitory regulation of esophageal smooth muscle is mediated by P2Y receptors and ATP-dependent potassium channels in rats |
| title_full | Purinergic inhibitory regulation of esophageal smooth muscle is mediated by P2Y receptors and ATP-dependent potassium channels in rats |
| title_fullStr | Purinergic inhibitory regulation of esophageal smooth muscle is mediated by P2Y receptors and ATP-dependent potassium channels in rats |
| title_full_unstemmed | Purinergic inhibitory regulation of esophageal smooth muscle is mediated by P2Y receptors and ATP-dependent potassium channels in rats |
| title_short | Purinergic inhibitory regulation of esophageal smooth muscle is mediated by P2Y receptors and ATP-dependent potassium channels in rats |
| title_sort | purinergic inhibitory regulation of esophageal smooth muscle is mediated by p2y receptors and atp dependent potassium channels in rats |
| topic | ATP-dependent potassium channel Esophagus P2 receptor Purine Smooth muscle |
| url | https://doi.org/10.1186/s12576-024-00916-5 |
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