In vitro antibacterial activity of Moringa oleifera leaf and seed extracts against antibiotic-resistant bacteria and clinical isolates
Abstract Background Resistance to antibiotic has increased extensively over the years, posing a serious threat to public health. Plants are promising sources of antibiotic-resistance inhibitors. The aim of this study is to investigate the antibacterial properties leaf and seed extracts of Moringa ol...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-07-01
|
| Series: | BMC Complementary Medicine and Therapies |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12906-025-05006-6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849344837765038080 |
|---|---|
| author | Sakyibea Apenteng-Takyiako Cecilia Afua Amoah Takyi Mary Anti Chama Mary-Magdalene Osei Eric Sampane Donkor Godfred Futagbi |
| author_facet | Sakyibea Apenteng-Takyiako Cecilia Afua Amoah Takyi Mary Anti Chama Mary-Magdalene Osei Eric Sampane Donkor Godfred Futagbi |
| author_sort | Sakyibea Apenteng-Takyiako |
| collection | DOAJ |
| description | Abstract Background Resistance to antibiotic has increased extensively over the years, posing a serious threat to public health. Plants are promising sources of antibiotic-resistance inhibitors. The aim of this study is to investigate the antibacterial properties leaf and seed extracts of Moringa oleifera against strains of pathogenic bacteria. Methods Eight extracts of M. oleifera leaves and seeds were prepared using four solvents; water, ethanol, ethyl acetate and petroleum ether, and evaluated against drug-resistant clinical isolates and standard laboratory strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa using disc diffusion method. Minimum inhibitory concentration of the extracts was determined by broth microdilution method. Results The results show that aqueous extracts of both leaves and seeds had higher zones of inhibition for all the isolates, including Methicillin-resistant S. aureus, E. coli ESBL, meropenem-resistant E. coli, than the rest of the extracts, with zones of inhibition ranging from 20.0 mm to 32.5 mm. Ethyl acetate seed extract also inhibited all the isolates except Meropenem-resistant E. coli. Likewise, ethyl acetate leaf extract inhibited all except the E. coli isolates and had significantly higher zones inhibition for almost all the isolates compared to the ethyl acetate seed extract. However, aqueous seed extract showed the lowest minimum inhibitory concentration against all bacteria strains, ranging from 50 mg/ml to 200 mg/ml. Conclusions The data indicates that aqueous and ethyl acetate extracts of M. oleifera, particularly aqueous seed extract, have the potential of capturing inhibitors against drug-resistant and clinical isolates of S. aureus, P. aeruginosa and E. coli. |
| format | Article |
| id | doaj-art-0cdb9db3a14b4743b49fc51454828d28 |
| institution | Kabale University |
| issn | 2662-7671 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj-art-0cdb9db3a14b4743b49fc51454828d282025-08-20T03:42:34ZengBMCBMC Complementary Medicine and Therapies2662-76712025-07-012511810.1186/s12906-025-05006-6In vitro antibacterial activity of Moringa oleifera leaf and seed extracts against antibiotic-resistant bacteria and clinical isolatesSakyibea Apenteng-Takyiako0Cecilia Afua Amoah Takyi1Mary Anti Chama2Mary-Magdalene Osei3Eric Sampane Donkor4Godfred Futagbi5Department of Animal Biology and conservation Science, School of Basic and Applied Sciences, University of GhanaDepartment of Animal Biology and conservation Science, School of Basic and Applied Sciences, University of GhanaDepartment of Chemistry, School of Basic and Applied Sciences, University of GhanaDepartment of Medical Microbiology, Medical School, University of Ghana, University of GhanaDepartment of Medical Microbiology, Medical School, University of Ghana, University of GhanaDepartment of Animal Biology and conservation Science, School of Basic and Applied Sciences, University of GhanaAbstract Background Resistance to antibiotic has increased extensively over the years, posing a serious threat to public health. Plants are promising sources of antibiotic-resistance inhibitors. The aim of this study is to investigate the antibacterial properties leaf and seed extracts of Moringa oleifera against strains of pathogenic bacteria. Methods Eight extracts of M. oleifera leaves and seeds were prepared using four solvents; water, ethanol, ethyl acetate and petroleum ether, and evaluated against drug-resistant clinical isolates and standard laboratory strains of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa using disc diffusion method. Minimum inhibitory concentration of the extracts was determined by broth microdilution method. Results The results show that aqueous extracts of both leaves and seeds had higher zones of inhibition for all the isolates, including Methicillin-resistant S. aureus, E. coli ESBL, meropenem-resistant E. coli, than the rest of the extracts, with zones of inhibition ranging from 20.0 mm to 32.5 mm. Ethyl acetate seed extract also inhibited all the isolates except Meropenem-resistant E. coli. Likewise, ethyl acetate leaf extract inhibited all except the E. coli isolates and had significantly higher zones inhibition for almost all the isolates compared to the ethyl acetate seed extract. However, aqueous seed extract showed the lowest minimum inhibitory concentration against all bacteria strains, ranging from 50 mg/ml to 200 mg/ml. Conclusions The data indicates that aqueous and ethyl acetate extracts of M. oleifera, particularly aqueous seed extract, have the potential of capturing inhibitors against drug-resistant and clinical isolates of S. aureus, P. aeruginosa and E. coli.https://doi.org/10.1186/s12906-025-05006-6MoringaAntimicrobial activityDrug-resistant bacteria |
| spellingShingle | Sakyibea Apenteng-Takyiako Cecilia Afua Amoah Takyi Mary Anti Chama Mary-Magdalene Osei Eric Sampane Donkor Godfred Futagbi In vitro antibacterial activity of Moringa oleifera leaf and seed extracts against antibiotic-resistant bacteria and clinical isolates BMC Complementary Medicine and Therapies Moringa Antimicrobial activity Drug-resistant bacteria |
| title | In vitro antibacterial activity of Moringa oleifera leaf and seed extracts against antibiotic-resistant bacteria and clinical isolates |
| title_full | In vitro antibacterial activity of Moringa oleifera leaf and seed extracts against antibiotic-resistant bacteria and clinical isolates |
| title_fullStr | In vitro antibacterial activity of Moringa oleifera leaf and seed extracts against antibiotic-resistant bacteria and clinical isolates |
| title_full_unstemmed | In vitro antibacterial activity of Moringa oleifera leaf and seed extracts against antibiotic-resistant bacteria and clinical isolates |
| title_short | In vitro antibacterial activity of Moringa oleifera leaf and seed extracts against antibiotic-resistant bacteria and clinical isolates |
| title_sort | in vitro antibacterial activity of moringa oleifera leaf and seed extracts against antibiotic resistant bacteria and clinical isolates |
| topic | Moringa Antimicrobial activity Drug-resistant bacteria |
| url | https://doi.org/10.1186/s12906-025-05006-6 |
| work_keys_str_mv | AT sakyibeaapentengtakyiako invitroantibacterialactivityofmoringaoleiferaleafandseedextractsagainstantibioticresistantbacteriaandclinicalisolates AT ceciliaafuaamoahtakyi invitroantibacterialactivityofmoringaoleiferaleafandseedextractsagainstantibioticresistantbacteriaandclinicalisolates AT maryantichama invitroantibacterialactivityofmoringaoleiferaleafandseedextractsagainstantibioticresistantbacteriaandclinicalisolates AT marymagdaleneosei invitroantibacterialactivityofmoringaoleiferaleafandseedextractsagainstantibioticresistantbacteriaandclinicalisolates AT ericsampanedonkor invitroantibacterialactivityofmoringaoleiferaleafandseedextractsagainstantibioticresistantbacteriaandclinicalisolates AT godfredfutagbi invitroantibacterialactivityofmoringaoleiferaleafandseedextractsagainstantibioticresistantbacteriaandclinicalisolates |