Investigating the Effect of Alcoholic Extract as Antimicrobial, Antioxidant, and Anticancer Drug Biosynthesized from Onion Allium Plant
Background and Objective: The use of chemical drugs results in substantial adverse effects and the development of drug resistance. Onion Allium cepa (O. Allium cepa) is one of the most well-known herbal treatments due to its ability to reduce inflammation and strengthen the body's immune system...
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| Format: | Article |
| Language: | English |
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Babol University of Medical Sciences
2024-03-01
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| Series: | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul |
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| Online Access: | http://jbums.org/article-1-11432-en.pdf |
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| author | AH Hameed EA Mohamed RH Sami BA Hasoon KF Al-Azawi NG Abdul Kareem Alawsi Z Abbas Fadhil S Mahdi Nasser |
| author_facet | AH Hameed EA Mohamed RH Sami BA Hasoon KF Al-Azawi NG Abdul Kareem Alawsi Z Abbas Fadhil S Mahdi Nasser |
| author_sort | AH Hameed |
| collection | DOAJ |
| description | Background and Objective: The use of chemical drugs results in substantial adverse effects and the development of drug resistance. Onion Allium cepa (O. Allium cepa) is one of the most well-known herbal treatments due to its ability to reduce inflammation and strengthen the body's immune system. The aim of this study is to investigate the effect of alcoholic extract as an antimicrobial, antioxidant and anticancer drug biosynthesized from Onion Allium.
Methods: In this laboratory study, the ethanolic extract of the onion Allium cepa plant was used to prepare the biosynthesis using a soxhlet with 75% ethanol. The UV/Vis spectrophotometry (UV-vis) and Fourier transmission infrared analysis (FTIR) were employed for characterization of ethanolic extract. Antibacterial activities of ethanolic extract against Escherichia coli (E. coli) and Staphylococcus aurous (S. aurous) were investigated. The cytotoxic effect of alcoholic extract on AMJ-13 breast cancer cell line at different concentrations (6.125, 12.5, 25, 50 and 100) micrograms/ml was evaluated by MTT method and DPPH was used to evaluate the antioxidant activity.
Findings: According to the results of Fourier transmission infrared analysis and phytochemical screening, numerous active substances, including polysaccharides, saponins, phenolic compounds, glycosides, and flavonoids were found in this extract. The antimicrobial activity test results revealed the largest inhibition zone in Escherichia coli as compared to Staphylococcus aureus. The AMJ-13 cell line's proliferation was greatly reduced by this extract in a concentration-dependent way when compared to the control, whereas this extract's cytotoxic activity increased with concentration. The findings demonstrated that Onion Allium cepa is a principally valuable source of powerful anti-oxidant.
Conclusion: According to the results of this study, ethanolic extract of the onion Allium cepa plant has antibacterial activity and reduced malignant cell lines' ability to proliferate and showed a strong antioxidant impact. |
| format | Article |
| id | doaj-art-0cd8ad4e26144716ab912731be510ffd |
| institution | OA Journals |
| issn | 1561-4107 2251-7170 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Babol University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul |
| spelling | doaj-art-0cd8ad4e26144716ab912731be510ffd2025-08-20T01:54:50ZengBabol University of Medical SciencesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul1561-41072251-71702024-03-012600Investigating the Effect of Alcoholic Extract as Antimicrobial, Antioxidant, and Anticancer Drug Biosynthesized from Onion Allium PlantAH Hameed0EA Mohamed1RH Sami2BA Hasoon3KF Al-Azawi4NG Abdul Kareem Alawsi5Z Abbas Fadhil6S Mahdi Nasser7 1.Department of Applied Sciences, School of Biotechnology, University of Technology, Baghdad, Iraq. 1.Department of Applied Sciences, School of Biotechnology, University of Technology, Baghdad, Iraq. 2.Departmentof Quality Assurance, University of Technology, Baghdad, Iraq. 1.Department of Applied Sciences, School of Biotechnology, University of Technology, Baghdad, Iraq. 1.Department of Applied Sciences, School of Biotechnology, University of Technology, Baghdad, Iraq. 1.Department of Applied Sciences, School of Biotechnology, University of Technology, Baghdad, Iraq. 1.Department of Applied Sciences, School of Biotechnology, University of Technology, Baghdad, Iraq. 1.Department of Applied Sciences, School of Biotechnology, University of Technology, Baghdad, Iraq. Background and Objective: The use of chemical drugs results in substantial adverse effects and the development of drug resistance. Onion Allium cepa (O. Allium cepa) is one of the most well-known herbal treatments due to its ability to reduce inflammation and strengthen the body's immune system. The aim of this study is to investigate the effect of alcoholic extract as an antimicrobial, antioxidant and anticancer drug biosynthesized from Onion Allium. Methods: In this laboratory study, the ethanolic extract of the onion Allium cepa plant was used to prepare the biosynthesis using a soxhlet with 75% ethanol. The UV/Vis spectrophotometry (UV-vis) and Fourier transmission infrared analysis (FTIR) were employed for characterization of ethanolic extract. Antibacterial activities of ethanolic extract against Escherichia coli (E. coli) and Staphylococcus aurous (S. aurous) were investigated. The cytotoxic effect of alcoholic extract on AMJ-13 breast cancer cell line at different concentrations (6.125, 12.5, 25, 50 and 100) micrograms/ml was evaluated by MTT method and DPPH was used to evaluate the antioxidant activity. Findings: According to the results of Fourier transmission infrared analysis and phytochemical screening, numerous active substances, including polysaccharides, saponins, phenolic compounds, glycosides, and flavonoids were found in this extract. The antimicrobial activity test results revealed the largest inhibition zone in Escherichia coli as compared to Staphylococcus aureus. The AMJ-13 cell line's proliferation was greatly reduced by this extract in a concentration-dependent way when compared to the control, whereas this extract's cytotoxic activity increased with concentration. The findings demonstrated that Onion Allium cepa is a principally valuable source of powerful anti-oxidant. Conclusion: According to the results of this study, ethanolic extract of the onion Allium cepa plant has antibacterial activity and reduced malignant cell lines' ability to proliferate and showed a strong antioxidant impact.http://jbums.org/article-1-11432-en.pdfonion alliumfourier transmission infrared analysisbiology activity. |
| spellingShingle | AH Hameed EA Mohamed RH Sami BA Hasoon KF Al-Azawi NG Abdul Kareem Alawsi Z Abbas Fadhil S Mahdi Nasser Investigating the Effect of Alcoholic Extract as Antimicrobial, Antioxidant, and Anticancer Drug Biosynthesized from Onion Allium Plant Majallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul onion allium fourier transmission infrared analysis biology activity. |
| title | Investigating the Effect of Alcoholic Extract as Antimicrobial, Antioxidant, and Anticancer Drug Biosynthesized from Onion Allium Plant |
| title_full | Investigating the Effect of Alcoholic Extract as Antimicrobial, Antioxidant, and Anticancer Drug Biosynthesized from Onion Allium Plant |
| title_fullStr | Investigating the Effect of Alcoholic Extract as Antimicrobial, Antioxidant, and Anticancer Drug Biosynthesized from Onion Allium Plant |
| title_full_unstemmed | Investigating the Effect of Alcoholic Extract as Antimicrobial, Antioxidant, and Anticancer Drug Biosynthesized from Onion Allium Plant |
| title_short | Investigating the Effect of Alcoholic Extract as Antimicrobial, Antioxidant, and Anticancer Drug Biosynthesized from Onion Allium Plant |
| title_sort | investigating the effect of alcoholic extract as antimicrobial antioxidant and anticancer drug biosynthesized from onion allium plant |
| topic | onion allium fourier transmission infrared analysis biology activity. |
| url | http://jbums.org/article-1-11432-en.pdf |
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