Mycorrhizal fungi and Fusarium species associated with vanilla in traditional management systems in Papantla, Veracruz, Mexico
Background: The Totonacapan region is recognized as the origin of vanilla cultivation (Vanilla planifolia Andrews), an orchid used for its gastronomic and medicinal values in rituals from pre-Hispanic times. Since that time, locals have cultivated vanilla using several traditional systems with diff...
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Sociedad Botánica de México, A. C.
2025-07-01
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| Series: | Botanical Sciences |
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| Online Access: | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/3704 |
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| author | Amanda Alejo-Viderique María Del Pilar Ortega-Larrocea Marcelo Rojas-Oropeza Nathalie Cabirol |
| author_facet | Amanda Alejo-Viderique María Del Pilar Ortega-Larrocea Marcelo Rojas-Oropeza Nathalie Cabirol |
| author_sort | Amanda Alejo-Viderique |
| collection | DOAJ |
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Background: The Totonacapan region is recognized as the origin of vanilla cultivation (Vanilla planifolia Andrews), an orchid used for its gastronomic and medicinal values in rituals from pre-Hispanic times. Since that time, locals have cultivated vanilla using several traditional systems with different management practices. This plant forms symbiotic associations with mycorrhizal fungi inside its roots, which serve as a source of nutrients.
Question: The objective of this study was to isolate and identify mycorrhizal fungi from traditional systems to search potential bioinoculants that enhance the organic and traditional cultivation among local communities.
Studied species: Vanilla planifolia Andrews.
Study site and dates: We sampled four management systems: Acahual (Traditional management, forest composed mainly of secondary vegetation), shade house (semi-technified cultivation system), under orange (Citrus sinensis L.) and pichoco (Erythrina spp.) trees in the region of Papantla, Veracruz, Mexico in 2022-2023.
Methods: The isolation was made from terrestrial roots, after the morphological and molecular characteristics of isolates by amplifying the ITS region.
Results: A total of ten strains were obtained from the four systems, which all correspond to the genus Tulasnella. At the same time, isolates of the genus Fusarium (F. falciforme and F. oxysporum f. sp. vanillae) were identified as endophytes and pathogenic species of vanilla.
Conclusions: The genus Tulasnella is the main mycorrhizal partner associated to vanilla in the Totonacapan cultivars; according to the phylogenetic analysis, seven of the isolated strains form a separate clade, suggesting that these strains are from the region of Papantla, Veracruz.
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| format | Article |
| id | doaj-art-0cd62d3b2d3c4bf487cab39ca2b8430b |
| institution | Kabale University |
| issn | 2007-4298 2007-4476 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Sociedad Botánica de México, A. C. |
| record_format | Article |
| series | Botanical Sciences |
| spelling | doaj-art-0cd62d3b2d3c4bf487cab39ca2b8430b2025-08-20T03:28:22ZengSociedad Botánica de México, A. C.Botanical Sciences2007-42982007-44762025-07-01103310.17129/botsci.3704Mycorrhizal fungi and Fusarium species associated with vanilla in traditional management systems in Papantla, Veracruz, MexicoAmanda Alejo-Viderique0https://orcid.org/0009-0006-6684-1672María Del Pilar Ortega-Larrocea1https://orcid.org/0000-0002-5062-8686Marcelo Rojas-Oropeza2https://orcid.org/0000-0002-8357-2674Nathalie Cabirol3https://orcid.org/0000-0001-7949-5904Grupo Ecología Microbiana Funcional del Suelo y Protección Ambiental, Departamento de Ecología y Recursos Naturales, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico CityDepartamento de Ciencias Ambientales y del Suelo, Instituto de Geología, Universidad Nacional Autónoma de México, Mexico CityGrupo Ecología Microbiana Funcional del Suelo y Protección Ambiental, Departamento de Ecología y Recursos Naturales, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico CityGrupo Ecología Microbiana Funcional del Suelo y Protección Ambiental, Departamento de Ecología y Recursos Naturales, Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City Background: The Totonacapan region is recognized as the origin of vanilla cultivation (Vanilla planifolia Andrews), an orchid used for its gastronomic and medicinal values in rituals from pre-Hispanic times. Since that time, locals have cultivated vanilla using several traditional systems with different management practices. This plant forms symbiotic associations with mycorrhizal fungi inside its roots, which serve as a source of nutrients. Question: The objective of this study was to isolate and identify mycorrhizal fungi from traditional systems to search potential bioinoculants that enhance the organic and traditional cultivation among local communities. Studied species: Vanilla planifolia Andrews. Study site and dates: We sampled four management systems: Acahual (Traditional management, forest composed mainly of secondary vegetation), shade house (semi-technified cultivation system), under orange (Citrus sinensis L.) and pichoco (Erythrina spp.) trees in the region of Papantla, Veracruz, Mexico in 2022-2023. Methods: The isolation was made from terrestrial roots, after the morphological and molecular characteristics of isolates by amplifying the ITS region. Results: A total of ten strains were obtained from the four systems, which all correspond to the genus Tulasnella. At the same time, isolates of the genus Fusarium (F. falciforme and F. oxysporum f. sp. vanillae) were identified as endophytes and pathogenic species of vanilla. Conclusions: The genus Tulasnella is the main mycorrhizal partner associated to vanilla in the Totonacapan cultivars; according to the phylogenetic analysis, seven of the isolated strains form a separate clade, suggesting that these strains are from the region of Papantla, Veracruz. https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/3704agroforestrybioinoculantsEpulorhizaFusariumTulasnella |
| spellingShingle | Amanda Alejo-Viderique María Del Pilar Ortega-Larrocea Marcelo Rojas-Oropeza Nathalie Cabirol Mycorrhizal fungi and Fusarium species associated with vanilla in traditional management systems in Papantla, Veracruz, Mexico Botanical Sciences agroforestry bioinoculants Epulorhiza Fusarium Tulasnella |
| title | Mycorrhizal fungi and Fusarium species associated with vanilla in traditional management systems in Papantla, Veracruz, Mexico |
| title_full | Mycorrhizal fungi and Fusarium species associated with vanilla in traditional management systems in Papantla, Veracruz, Mexico |
| title_fullStr | Mycorrhizal fungi and Fusarium species associated with vanilla in traditional management systems in Papantla, Veracruz, Mexico |
| title_full_unstemmed | Mycorrhizal fungi and Fusarium species associated with vanilla in traditional management systems in Papantla, Veracruz, Mexico |
| title_short | Mycorrhizal fungi and Fusarium species associated with vanilla in traditional management systems in Papantla, Veracruz, Mexico |
| title_sort | mycorrhizal fungi and fusarium species associated with vanilla in traditional management systems in papantla veracruz mexico |
| topic | agroforestry bioinoculants Epulorhiza Fusarium Tulasnella |
| url | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/3704 |
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