GRASPs link Reelin to the Golgi during neocortical development to control neuronal migration and dendritogenesis

Abstract Reelin serves as a crucial regulator of brain organogenesis, playing a significant role in neuronal positioning and dendritogenesis. At subcellular level, it influences the translocation and remodeling of the Golgi apparatus. Despite its importance, the mechanisms by which Reelin governs th...

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Main Authors: Elisa Calvo-Jiménez, Kirsten Stam, Angélique Jossi, Yves Jossin
Format: Article
Language:English
Published: Nature Portfolio 2025-04-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-08014-x
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author Elisa Calvo-Jiménez
Kirsten Stam
Angélique Jossi
Yves Jossin
author_facet Elisa Calvo-Jiménez
Kirsten Stam
Angélique Jossi
Yves Jossin
author_sort Elisa Calvo-Jiménez
collection DOAJ
description Abstract Reelin serves as a crucial regulator of brain organogenesis, playing a significant role in neuronal positioning and dendritogenesis. At subcellular level, it influences the translocation and remodeling of the Golgi apparatus. Despite its importance, the mechanisms by which Reelin governs the Golgi during neuronal migration and dendrite formation remain largely unknown. This study reveals that Reelin promotes de novo translation of Golgi Re-Assembly Stacking Proteins (GRASPs), which are essential for the functions of Reelin on cortical neurons. Downregulation of GRASPs in migrating excitatory neurons of the embryonic neocortex leads to disoriented cells during the multipolar phase of migration and an aberrant leading process length during locomotion. Postnatally, it results in mislocalized neurons displaying a disorganized Golgi structure and an improperly oriented, underdeveloped apical dendrite. Our findings position GRASPs and their role in Golgi morphology modulation as novel contributors to the Reelin-mediated processes during embryonic development of the mammalian neocortex.
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spelling doaj-art-0ccd24f90739436fa1d75cbf1f1a37f42025-08-20T03:07:44ZengNature PortfolioCommunications Biology2399-36422025-04-018111410.1038/s42003-025-08014-xGRASPs link Reelin to the Golgi during neocortical development to control neuronal migration and dendritogenesisElisa Calvo-Jiménez0Kirsten Stam1Angélique Jossi2Yves Jossin3Laboratory of Mammalian Development & Cell Biology, Institute of Neuroscience, Université Catholique de LouvainLaboratory of Mammalian Development & Cell Biology, Institute of Neuroscience, Université Catholique de LouvainLaboratory of Mammalian Development & Cell Biology, Institute of Neuroscience, Université Catholique de LouvainLaboratory of Mammalian Development & Cell Biology, Institute of Neuroscience, Université Catholique de LouvainAbstract Reelin serves as a crucial regulator of brain organogenesis, playing a significant role in neuronal positioning and dendritogenesis. At subcellular level, it influences the translocation and remodeling of the Golgi apparatus. Despite its importance, the mechanisms by which Reelin governs the Golgi during neuronal migration and dendrite formation remain largely unknown. This study reveals that Reelin promotes de novo translation of Golgi Re-Assembly Stacking Proteins (GRASPs), which are essential for the functions of Reelin on cortical neurons. Downregulation of GRASPs in migrating excitatory neurons of the embryonic neocortex leads to disoriented cells during the multipolar phase of migration and an aberrant leading process length during locomotion. Postnatally, it results in mislocalized neurons displaying a disorganized Golgi structure and an improperly oriented, underdeveloped apical dendrite. Our findings position GRASPs and their role in Golgi morphology modulation as novel contributors to the Reelin-mediated processes during embryonic development of the mammalian neocortex.https://doi.org/10.1038/s42003-025-08014-x
spellingShingle Elisa Calvo-Jiménez
Kirsten Stam
Angélique Jossi
Yves Jossin
GRASPs link Reelin to the Golgi during neocortical development to control neuronal migration and dendritogenesis
Communications Biology
title GRASPs link Reelin to the Golgi during neocortical development to control neuronal migration and dendritogenesis
title_full GRASPs link Reelin to the Golgi during neocortical development to control neuronal migration and dendritogenesis
title_fullStr GRASPs link Reelin to the Golgi during neocortical development to control neuronal migration and dendritogenesis
title_full_unstemmed GRASPs link Reelin to the Golgi during neocortical development to control neuronal migration and dendritogenesis
title_short GRASPs link Reelin to the Golgi during neocortical development to control neuronal migration and dendritogenesis
title_sort grasps link reelin to the golgi during neocortical development to control neuronal migration and dendritogenesis
url https://doi.org/10.1038/s42003-025-08014-x
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AT angeliquejossi graspslinkreelintothegolgiduringneocorticaldevelopmenttocontrolneuronalmigrationanddendritogenesis
AT yvesjossin graspslinkreelintothegolgiduringneocorticaldevelopmenttocontrolneuronalmigrationanddendritogenesis