Comparison Between the Wong Incision and Stromal Hydration of Corneal Incisions in Phacoemulsification
Objective: To determine the efficacy of the Wong incision in providing wound seal compared to stromal wall hydration in clear cornea phacoemulsification in cadaveric porcine eyes. Method: This was an in vitro comparative experimental study using ten porcine eyes. All eyes were randomly assigned t...
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Philippine Academy of Ophthalmology
2012-12-01
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| Series: | Philippine Journal of Ophthalmology |
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| Online Access: | https://paojournal.com/index.php/pjo/article/view/243 |
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| author | Raymond Nelson Regalado, MD Jay Marianito Vicencio, MD Ruben Lim Bon Siong, MD Lee Verzosa, MD |
| author_facet | Raymond Nelson Regalado, MD Jay Marianito Vicencio, MD Ruben Lim Bon Siong, MD Lee Verzosa, MD |
| author_sort | Raymond Nelson Regalado, MD |
| collection | DOAJ |
| description | Objective: To determine the efficacy of the Wong incision in providing wound seal compared to stromal wall hydration in clear cornea phacoemulsification in cadaveric porcine eyes.
Method: This was an in vitro comparative experimental study using ten porcine eyes. All eyes were randomly assigned to the stromal wall hydration (control) or the Wong incision group (experimental). A side port was made and the anterior chamber formed with viscoelastic device. The main incision was made 180 degrees away. In the experimental group, a Wong incision was made first anterior to the main incision. Phacoemulsification surgery with IOL insertion was simulated. The main incision was sealed by hydration. The anterior chamber (AC) was infused with balanced salt solution (BSS) through an AC maintainer and leakage of fluid from the main incision was assessed. Samples from the AC were taken before and after tryphan blue drip and were sent for analysis by UV spectrophotometry. Trypan blue was dripped over the main incision and the whole eyeball was sent for histopathology.
Results: There was a significant increase in density from the pre-dye to the post-dye AC samples of the control (0.0052 to 0.0074, p=0.01) and the experimental groups (0.0076 to 0.0094, p=0.02), although the final samples showed an optical density comparable to pure BSS, indicating that there was no significant amount of trypan blue detected in both groups. On histology, trypan blue staining was not seen in the incision tracts of both groups. After infusing the AC with BSS, there was outward wound leakage in all eyes of the control group and none in the experimental group.
Conclusion: The Wong incision was as effective as the lateral stromal wall hydration in preventing fluid influx. Furthermore, the Wong incision showed a more stable wound seal against outward wound leakage in an in-vitro porcine model of clear corneal phacoemulsification. |
| format | Article |
| id | doaj-art-0cc18fb75c334c9d8fe5b10f7814a8bb |
| institution | Kabale University |
| issn | 0031-7659 |
| language | English |
| publishDate | 2012-12-01 |
| publisher | Philippine Academy of Ophthalmology |
| record_format | Article |
| series | Philippine Journal of Ophthalmology |
| spelling | doaj-art-0cc18fb75c334c9d8fe5b10f7814a8bb2025-08-20T03:41:56ZengPhilippine Academy of OphthalmologyPhilippine Journal of Ophthalmology0031-76592012-12-0137297103243Comparison Between the Wong Incision and Stromal Hydration of Corneal Incisions in PhacoemulsificationRaymond Nelson Regalado, MD0Jay Marianito Vicencio, MD1Ruben Lim Bon Siong, MD2Lee Verzosa, MD3International Eye Institute, St. Luke’s Medical Center Quezon City, 1102, PhilippinesInternational Eye Institute, St. Luke’s Medical Center Quezon City, 1102, PhilippinesInternational Eye Institute, St. Luke’s Medical Center Quezon City, 1102, PhilippinesInternational Eye Institute, St. Luke’s Medical Center Quezon City, 1102, PhilippinesObjective: To determine the efficacy of the Wong incision in providing wound seal compared to stromal wall hydration in clear cornea phacoemulsification in cadaveric porcine eyes. Method: This was an in vitro comparative experimental study using ten porcine eyes. All eyes were randomly assigned to the stromal wall hydration (control) or the Wong incision group (experimental). A side port was made and the anterior chamber formed with viscoelastic device. The main incision was made 180 degrees away. In the experimental group, a Wong incision was made first anterior to the main incision. Phacoemulsification surgery with IOL insertion was simulated. The main incision was sealed by hydration. The anterior chamber (AC) was infused with balanced salt solution (BSS) through an AC maintainer and leakage of fluid from the main incision was assessed. Samples from the AC were taken before and after tryphan blue drip and were sent for analysis by UV spectrophotometry. Trypan blue was dripped over the main incision and the whole eyeball was sent for histopathology. Results: There was a significant increase in density from the pre-dye to the post-dye AC samples of the control (0.0052 to 0.0074, p=0.01) and the experimental groups (0.0076 to 0.0094, p=0.02), although the final samples showed an optical density comparable to pure BSS, indicating that there was no significant amount of trypan blue detected in both groups. On histology, trypan blue staining was not seen in the incision tracts of both groups. After infusing the AC with BSS, there was outward wound leakage in all eyes of the control group and none in the experimental group. Conclusion: The Wong incision was as effective as the lateral stromal wall hydration in preventing fluid influx. Furthermore, the Wong incision showed a more stable wound seal against outward wound leakage in an in-vitro porcine model of clear corneal phacoemulsification.https://paojournal.com/index.php/pjo/article/view/243wong incisionsupraincisional hydration pocketstromal hydrationclear corneal incisionphaco-emulsificationwound leak |
| spellingShingle | Raymond Nelson Regalado, MD Jay Marianito Vicencio, MD Ruben Lim Bon Siong, MD Lee Verzosa, MD Comparison Between the Wong Incision and Stromal Hydration of Corneal Incisions in Phacoemulsification Philippine Journal of Ophthalmology wong incision supraincisional hydration pocket stromal hydration clear corneal incision phaco-emulsification wound leak |
| title | Comparison Between the Wong Incision and Stromal Hydration of Corneal Incisions in Phacoemulsification |
| title_full | Comparison Between the Wong Incision and Stromal Hydration of Corneal Incisions in Phacoemulsification |
| title_fullStr | Comparison Between the Wong Incision and Stromal Hydration of Corneal Incisions in Phacoemulsification |
| title_full_unstemmed | Comparison Between the Wong Incision and Stromal Hydration of Corneal Incisions in Phacoemulsification |
| title_short | Comparison Between the Wong Incision and Stromal Hydration of Corneal Incisions in Phacoemulsification |
| title_sort | comparison between the wong incision and stromal hydration of corneal incisions in phacoemulsification |
| topic | wong incision supraincisional hydration pocket stromal hydration clear corneal incision phaco-emulsification wound leak |
| url | https://paojournal.com/index.php/pjo/article/view/243 |
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