Agarwood extract improves psoriatic-like inflammation in HaCaT cells by regulating NF-κB pathway
Objective To investigate the effect and mechanism of agarwood extract (AE) on tumor necrosis factor-α (TNF-α)-induced psoriatic-like inflammation model of keratinocytes (HaCaT). Methods The psoriatic-like inflammation model of HaCaT cells was induced by 40 ng/mL TNF-α, and then the cells were treate...
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Editorial Office of Journal of Guangxi Medical University
2025-04-01
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| Series: | Guangxi Yike Daxue xuebao |
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| Online Access: | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2025.02.012 |
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| author | HUANG Weiwei ZHANG Qi ZHAO Binbin LI Wenyu GUO Ting WEN Sijian CAO Cunwei LIN Youkun |
| author_facet | HUANG Weiwei ZHANG Qi ZHAO Binbin LI Wenyu GUO Ting WEN Sijian CAO Cunwei LIN Youkun |
| author_sort | HUANG Weiwei |
| collection | DOAJ |
| description | Objective To investigate the effect and mechanism of agarwood extract (AE) on tumor necrosis factor-α (TNF-α)-induced psoriatic-like inflammation model of keratinocytes (HaCaT). Methods The psoriatic-like inflammation model of HaCaT cells was induced by 40 ng/mL TNF-α, and then the cells were treated with AE at concentrations of 16 μg/mL (low concentration), 24 μg/mL (medium concentration), 32 μg/mL (high concentration). Cell counting kit-8 (CCK-8) assay was used to detect the viability of HaCaT cells. Flow cytometry was applied to detect the apoptosis of HaCaT cells. Reverse transcription-quantitative polymerase chain reaction (RTqPCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the mRNA expression and secretion levels of various inflammatory mediators, as well as that of inhibitor kappa B alpha (IκBα). Western blotting was used to detect the expression of key proteins in the NF-κB pathway. Results After 40 ng/mL TNF-α stimulation, the viability of HaCaT cells was increased (P < 0.01), the early apoptosis rate and IκBα mRNA level were decreased (P < 0.05), and the mRNA expression levels and release of interleukin-6 (IL-6), interleukin-1β (IL-1β), interleukin17A (IL-17A), TNF-α, chemokine ligand 20 (CCL20) in cells were increased (P < 0.05). The expres‐sion level of phosphorylated nuclear factor-κB p65 (p-p65) protein was increased (P < 0.01). After a certain concentration of AE intervention, the viability of HaCaT cells was decreased (P < 0.01). Treatment with low, medium and high concentrations of AE in TNF-α pretreated HaCaT cells increased the early apoptosis rate and the mRNA-expression level of IκBα (P < 0.01). Meanwhile, it decreased the mRNA expression and release of IL-6, IL-1β, IL-17A, TNF-α and CCL20 (P < 0.05), as well as the p-p65 protein level (P < 0.01). Conclusion AE can inhibit the phosphorylation of p65 protein in the NF-κB pathway, thereby reducing the release of inflammatory factors and chemokines in the psoriatic-like inflammatory model of HaCaT cells, inducing apoptosis, and improving the inflammatory response. |
| format | Article |
| id | doaj-art-0cbef2a6dfaf4fe3be92a3401935f7f2 |
| institution | DOAJ |
| issn | 1005-930X |
| language | zho |
| publishDate | 2025-04-01 |
| publisher | Editorial Office of Journal of Guangxi Medical University |
| record_format | Article |
| series | Guangxi Yike Daxue xuebao |
| spelling | doaj-art-0cbef2a6dfaf4fe3be92a3401935f7f22025-08-20T02:57:36ZzhoEditorial Office of Journal of Guangxi Medical UniversityGuangxi Yike Daxue xuebao1005-930X2025-04-0142224725410.16190/j.cnki.45-1211/r.2025.02.012gxykdxxb-42-2-247Agarwood extract improves psoriatic-like inflammation in HaCaT cells by regulating NF-κB pathwayHUANG Weiwei0ZHANG Qi1ZHAO Binbin2LI Wenyu3GUO Ting4WEN Sijian5CAO Cunwei6LIN Youkun7Department of Dermatology and Venereology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Dermatology and Venereology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaGuangxi Helanmo Biotechnology Co., Ltd., Nanning 530219, ChinaDepartment of Dermatology and Venereology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Dermatology and Venereology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Dermatology and Venereology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Dermatology and Venereology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Dermatology and Venereology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaObjective To investigate the effect and mechanism of agarwood extract (AE) on tumor necrosis factor-α (TNF-α)-induced psoriatic-like inflammation model of keratinocytes (HaCaT). Methods The psoriatic-like inflammation model of HaCaT cells was induced by 40 ng/mL TNF-α, and then the cells were treated with AE at concentrations of 16 μg/mL (low concentration), 24 μg/mL (medium concentration), 32 μg/mL (high concentration). Cell counting kit-8 (CCK-8) assay was used to detect the viability of HaCaT cells. Flow cytometry was applied to detect the apoptosis of HaCaT cells. Reverse transcription-quantitative polymerase chain reaction (RTqPCR) and enzyme-linked immunosorbent assay (ELISA) were used to detect the mRNA expression and secretion levels of various inflammatory mediators, as well as that of inhibitor kappa B alpha (IκBα). Western blotting was used to detect the expression of key proteins in the NF-κB pathway. Results After 40 ng/mL TNF-α stimulation, the viability of HaCaT cells was increased (P < 0.01), the early apoptosis rate and IκBα mRNA level were decreased (P < 0.05), and the mRNA expression levels and release of interleukin-6 (IL-6), interleukin-1β (IL-1β), interleukin17A (IL-17A), TNF-α, chemokine ligand 20 (CCL20) in cells were increased (P < 0.05). The expres‐sion level of phosphorylated nuclear factor-κB p65 (p-p65) protein was increased (P < 0.01). After a certain concentration of AE intervention, the viability of HaCaT cells was decreased (P < 0.01). Treatment with low, medium and high concentrations of AE in TNF-α pretreated HaCaT cells increased the early apoptosis rate and the mRNA-expression level of IκBα (P < 0.01). Meanwhile, it decreased the mRNA expression and release of IL-6, IL-1β, IL-17A, TNF-α and CCL20 (P < 0.05), as well as the p-p65 protein level (P < 0.01). Conclusion AE can inhibit the phosphorylation of p65 protein in the NF-κB pathway, thereby reducing the release of inflammatory factors and chemokines in the psoriatic-like inflammatory model of HaCaT cells, inducing apoptosis, and improving the inflammatory response.https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2025.02.012agarwood extractpsoriasishacat cellsnf-κb signaling pathwayinflammatory factorsapoptosis |
| spellingShingle | HUANG Weiwei ZHANG Qi ZHAO Binbin LI Wenyu GUO Ting WEN Sijian CAO Cunwei LIN Youkun Agarwood extract improves psoriatic-like inflammation in HaCaT cells by regulating NF-κB pathway Guangxi Yike Daxue xuebao agarwood extract psoriasis hacat cells nf-κb signaling pathway inflammatory factors apoptosis |
| title | Agarwood extract improves psoriatic-like inflammation in HaCaT cells by regulating NF-κB pathway |
| title_full | Agarwood extract improves psoriatic-like inflammation in HaCaT cells by regulating NF-κB pathway |
| title_fullStr | Agarwood extract improves psoriatic-like inflammation in HaCaT cells by regulating NF-κB pathway |
| title_full_unstemmed | Agarwood extract improves psoriatic-like inflammation in HaCaT cells by regulating NF-κB pathway |
| title_short | Agarwood extract improves psoriatic-like inflammation in HaCaT cells by regulating NF-κB pathway |
| title_sort | agarwood extract improves psoriatic like inflammation in hacat cells by regulating nf κb pathway |
| topic | agarwood extract psoriasis hacat cells nf-κb signaling pathway inflammatory factors apoptosis |
| url | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2025.02.012 |
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