Equity in protection: bridging global data gaps for an EBV vaccine—a systematic review and meta-analysis

Introduction Epstein–Barr virus (EBV) is linked to multiple malignancies and autoimmune conditions, with different disease burdens globally. Pharmaceutical companies and researchers are placing substantial investment in the development of EBV vaccines. To ensure optimal vaccine roll-out, particularl...

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Main Authors: Helen R Stagg, Ting Shi, Simran Kapoor, Marisa D Muckian, Vesa Qarkaxhija, Tomos Morgan
Format: Article
Language:English
Published: BMJ Publishing Group 2025-08-01
Series:BMJ Global Health
Online Access:https://gh.bmj.com/content/10/8/e015534.full
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author Helen R Stagg
Ting Shi
Simran Kapoor
Marisa D Muckian
Vesa Qarkaxhija
Tomos Morgan
author_facet Helen R Stagg
Ting Shi
Simran Kapoor
Marisa D Muckian
Vesa Qarkaxhija
Tomos Morgan
author_sort Helen R Stagg
collection DOAJ
description Introduction Epstein–Barr virus (EBV) is linked to multiple malignancies and autoimmune conditions, with different disease burdens globally. Pharmaceutical companies and researchers are placing substantial investment in the development of EBV vaccines. To ensure optimal vaccine roll-out, particularly in resource-limited settings, it is essential to have data on the age at acquisition of EBV. This study aimed to systematically review and meta-analyse seroprevalence by age and country, WHO region and country income level, identify knowledge gaps, and determine an approach to bridge these gaps.Methods MEDLINE, Embase and Web of Science were searched on 22 March 2022 for studies that measured EBV seroprevalence by age. An updated search was conducted on 22 October 2022. There were no language restrictions. Papers were assessed for quality using an adapted version of the Downs and Black checklist. Seroprevalence by age was estimated using a fixed-effect (country) or random-effects (WHO region and income) meta-analysis. This review has been registered on PROSPERO (CRD42022349900).Results Only one country (USA) had enough data for a country meta-analysis. WHO regional analyses revealed the Western Pacific region to have a higher seroprevalence in younger age groups than other WHO regions. Country income level better explained seroprevalence trends per age. Middle-income countries displayed a quicker rise to balance seroprevalence than high-income countries, with a 30% absolute increase in 0- to 4-year-olds in middle-income than in high-income countries (59% [95% CI 28 to 91%, I2=99%] vs 29% [95% CI 16 to 41%, I2=99%]).Conclusion This first meta-analysis producing estimates of EBV seroprevalence by age provides crucial information to guide governments when using a vaccine for EBV. However, data variability and limited consistency of methodologies and EBV seroprevalence measurements hindered comprehensive meta-analyses across all WHO regions and countries. This study provides an interim framework for the extrapolation of seroprevalence using country-specific income levels to aid vaccine roll-out decisions.PROSPERO registration number CRD42022349900
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spelling doaj-art-0caea8ff7263401aa00e898101545bda2025-08-20T03:41:34ZengBMJ Publishing GroupBMJ Global Health2059-79082025-08-0110810.1136/bmjgh-2024-015534Equity in protection: bridging global data gaps for an EBV vaccine—a systematic review and meta-analysisHelen R Stagg0Ting Shi1Simran Kapoor2Marisa D Muckian3Vesa Qarkaxhija4Tomos Morgan5Department of Infectious Disease Epidemiology, London School of Hygiene & Tropical Medicine, London, UKPopulation Health Sciences and Informatics, Usher Institute, The University of Edinburgh, Edinburgh, UKDivision of Reproductive and Developmental Sciences, The University of Edinburgh, Edinburgh, UKDepartment of Infectious Disease Epidemiology, London School of Hygiene & Tropical Medicine, London, UKOld Medical School, The University of Edinburgh, UK, Edinburgh, UKOld Medical School, The University of Edinburgh, UK, Edinburgh, UKIntroduction Epstein–Barr virus (EBV) is linked to multiple malignancies and autoimmune conditions, with different disease burdens globally. Pharmaceutical companies and researchers are placing substantial investment in the development of EBV vaccines. To ensure optimal vaccine roll-out, particularly in resource-limited settings, it is essential to have data on the age at acquisition of EBV. This study aimed to systematically review and meta-analyse seroprevalence by age and country, WHO region and country income level, identify knowledge gaps, and determine an approach to bridge these gaps.Methods MEDLINE, Embase and Web of Science were searched on 22 March 2022 for studies that measured EBV seroprevalence by age. An updated search was conducted on 22 October 2022. There were no language restrictions. Papers were assessed for quality using an adapted version of the Downs and Black checklist. Seroprevalence by age was estimated using a fixed-effect (country) or random-effects (WHO region and income) meta-analysis. This review has been registered on PROSPERO (CRD42022349900).Results Only one country (USA) had enough data for a country meta-analysis. WHO regional analyses revealed the Western Pacific region to have a higher seroprevalence in younger age groups than other WHO regions. Country income level better explained seroprevalence trends per age. Middle-income countries displayed a quicker rise to balance seroprevalence than high-income countries, with a 30% absolute increase in 0- to 4-year-olds in middle-income than in high-income countries (59% [95% CI 28 to 91%, I2=99%] vs 29% [95% CI 16 to 41%, I2=99%]).Conclusion This first meta-analysis producing estimates of EBV seroprevalence by age provides crucial information to guide governments when using a vaccine for EBV. However, data variability and limited consistency of methodologies and EBV seroprevalence measurements hindered comprehensive meta-analyses across all WHO regions and countries. This study provides an interim framework for the extrapolation of seroprevalence using country-specific income levels to aid vaccine roll-out decisions.PROSPERO registration number CRD42022349900https://gh.bmj.com/content/10/8/e015534.full
spellingShingle Helen R Stagg
Ting Shi
Simran Kapoor
Marisa D Muckian
Vesa Qarkaxhija
Tomos Morgan
Equity in protection: bridging global data gaps for an EBV vaccine—a systematic review and meta-analysis
BMJ Global Health
title Equity in protection: bridging global data gaps for an EBV vaccine—a systematic review and meta-analysis
title_full Equity in protection: bridging global data gaps for an EBV vaccine—a systematic review and meta-analysis
title_fullStr Equity in protection: bridging global data gaps for an EBV vaccine—a systematic review and meta-analysis
title_full_unstemmed Equity in protection: bridging global data gaps for an EBV vaccine—a systematic review and meta-analysis
title_short Equity in protection: bridging global data gaps for an EBV vaccine—a systematic review and meta-analysis
title_sort equity in protection bridging global data gaps for an ebv vaccine a systematic review and meta analysis
url https://gh.bmj.com/content/10/8/e015534.full
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