Fungi-Kcr: a language model for predicting lysine crotonylation in pathogenic fungal proteins

Fungi-Kcr: a language model for predicting lysine crotonylation in pathogenic fungal proteins

IntroductionLysine crotonylation (Kcr) is an important post-translational modification (PTM) of proteins, playing a key role in regulating various biological processes in pathogenic fungi. However, the experimental identification of Kcr sites remains challenging due to the high cost and time-consumi...

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Bibliographic Details
Main Authors: Yong-Zi Chen, Xiaofeng Wang, Zhuo-Zhi Wang, Haixin Li
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-07-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
lysine crotonylation
fungal proteins
language model
pathogen
post-translational modifications
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2025.1615443/full
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Summary:IntroductionLysine crotonylation (Kcr) is an important post-translational modification (PTM) of proteins, playing a key role in regulating various biological processes in pathogenic fungi. However, the experimental identification of Kcr sites remains challenging due to the high cost and time-consuming nature of mass spectrometry-based techniques.MethodsTo address this limitation, we developed Fungi-Kcr, a deep learning-based model designed to predict Kcr modification sites in fungal proteins. The model integrates convolutional neural networks (CNN), gated recurrent units (GRU), and word embedding to effectively capture both local and long-range sequence dependencies.ResultsComprehensive evaluations, including ten-fold cross-validation and independent testing, demonstrate that Fungi-Kcr achieves superior predictive performance compared to conventional machine learning models. Moreover, our results indicate that a general predictive model performs better than species-specific models. DiscussionThe proposed model provides a valuable computational tool for the large-scale identification of Kcr sites, contributing to a deeper understanding of fungal pathogenesis and potential therapeutic targets. The source code and dataset for Fungi-Kcr are available at https://github.com/zayra77/Fungi-Kcr.
ISSN:2235-2988

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