Intracortical Administration of the Complement C3 Receptor Antagonist Trifluoroacetate Modulates Microglia Reaction after Brain Injury
Worldwide, millions of individuals suffer an ischemic stroke each year, causing major disability, especially in the elderly, where stroke is the number one cause of disability. However, to date, no effective therapy exists that targets the functional recovery after stroke. After necrosis, neuroinfla...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2019/1071036 |
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| author | Roxana Surugiu Bogdan Catalin Danut Dumbrava Andrei Gresita Denisa Greta Olaru Dirk M. Hermann Aurel Popa-Wagner |
| author_facet | Roxana Surugiu Bogdan Catalin Danut Dumbrava Andrei Gresita Denisa Greta Olaru Dirk M. Hermann Aurel Popa-Wagner |
| author_sort | Roxana Surugiu |
| collection | DOAJ |
| description | Worldwide, millions of individuals suffer an ischemic stroke each year, causing major disability, especially in the elderly, where stroke is the number one cause of disability. However, to date, no effective therapy exists that targets the functional recovery after stroke. After necrosis, neuroinflammation is a common feature of the acute stroke and a major obstacle to tissue restoration. In the lesioned area, the dying neurons release chemotactic signals, such as fractalkine/CX3CL1, which evoke “eat-me” signals that are recognized by microglia expressing complement C3a receptor (C3aR), resulting in phagocytosis of the dying but still viable neurons, known as secondary phagocytosis. Using a mouse model of stroke and two-photon microscopy, we aimed to attenuate poststroke phagocytosis of the dying but still viable neurons by using SB 290157, an antagonist of C3aR. We found that intracortical administration of SB 290157 reduced the number of inflammatory microglial cells expressing ED1 and Iba1 antigens at the lesion site. We could show, in vivo, that two days after a needle-induced cortical lesion there were less microglial cells present around the injury site, displaying less high-order branches and an increase in the lower order ones, suggesting an attenuated phagocytic phenotype in treated animals as compared with controls. We conclude that the C3aR antagonist, SB 290157, may be used in the future to limit the neuronal death by limiting secondary phagocytosis after stroke. |
| format | Article |
| id | doaj-art-0ca882c8d3004c24bfbbd8525749cf65 |
| institution | OA Journals |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-0ca882c8d3004c24bfbbd8525749cf652025-08-20T02:03:04ZengWileyNeural Plasticity2090-59041687-54432019-01-01201910.1155/2019/10710361071036Intracortical Administration of the Complement C3 Receptor Antagonist Trifluoroacetate Modulates Microglia Reaction after Brain InjuryRoxana Surugiu0Bogdan Catalin1Danut Dumbrava2Andrei Gresita3Denisa Greta Olaru4Dirk M. Hermann5Aurel Popa-Wagner6Center of Clinical and Experimental Medicine, University of Medicine and Pharmacy of Craiova, Craiova, RomaniaExperimental Research Centre for Normal and Pathological Aging, University of Medicine and Pharmacy of Craiova, RomaniaCenter of Clinical and Experimental Medicine, University of Medicine and Pharmacy of Craiova, Craiova, RomaniaCenter of Clinical and Experimental Medicine, University of Medicine and Pharmacy of Craiova, Craiova, RomaniaDepartment of Anatomy, University of Medicine and Pharmacy of Craiova, Craiova, RomaniaChair of Vascular Neurology, Dementia and Ageing Research, Department of Neurology, University of Duisburg-Essen, University Hospital Essen, GermanyCenter of Clinical and Experimental Medicine, University of Medicine and Pharmacy of Craiova, Craiova, RomaniaWorldwide, millions of individuals suffer an ischemic stroke each year, causing major disability, especially in the elderly, where stroke is the number one cause of disability. However, to date, no effective therapy exists that targets the functional recovery after stroke. After necrosis, neuroinflammation is a common feature of the acute stroke and a major obstacle to tissue restoration. In the lesioned area, the dying neurons release chemotactic signals, such as fractalkine/CX3CL1, which evoke “eat-me” signals that are recognized by microglia expressing complement C3a receptor (C3aR), resulting in phagocytosis of the dying but still viable neurons, known as secondary phagocytosis. Using a mouse model of stroke and two-photon microscopy, we aimed to attenuate poststroke phagocytosis of the dying but still viable neurons by using SB 290157, an antagonist of C3aR. We found that intracortical administration of SB 290157 reduced the number of inflammatory microglial cells expressing ED1 and Iba1 antigens at the lesion site. We could show, in vivo, that two days after a needle-induced cortical lesion there were less microglial cells present around the injury site, displaying less high-order branches and an increase in the lower order ones, suggesting an attenuated phagocytic phenotype in treated animals as compared with controls. We conclude that the C3aR antagonist, SB 290157, may be used in the future to limit the neuronal death by limiting secondary phagocytosis after stroke.http://dx.doi.org/10.1155/2019/1071036 |
| spellingShingle | Roxana Surugiu Bogdan Catalin Danut Dumbrava Andrei Gresita Denisa Greta Olaru Dirk M. Hermann Aurel Popa-Wagner Intracortical Administration of the Complement C3 Receptor Antagonist Trifluoroacetate Modulates Microglia Reaction after Brain Injury Neural Plasticity |
| title | Intracortical Administration of the Complement C3 Receptor Antagonist Trifluoroacetate Modulates Microglia Reaction after Brain Injury |
| title_full | Intracortical Administration of the Complement C3 Receptor Antagonist Trifluoroacetate Modulates Microglia Reaction after Brain Injury |
| title_fullStr | Intracortical Administration of the Complement C3 Receptor Antagonist Trifluoroacetate Modulates Microglia Reaction after Brain Injury |
| title_full_unstemmed | Intracortical Administration of the Complement C3 Receptor Antagonist Trifluoroacetate Modulates Microglia Reaction after Brain Injury |
| title_short | Intracortical Administration of the Complement C3 Receptor Antagonist Trifluoroacetate Modulates Microglia Reaction after Brain Injury |
| title_sort | intracortical administration of the complement c3 receptor antagonist trifluoroacetate modulates microglia reaction after brain injury |
| url | http://dx.doi.org/10.1155/2019/1071036 |
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