Molecular Mechanism of VSV-Vectored ASFV Vaccine Activating Immune Response in DCs
The vesicular stomatitis virus (VSV)-vectored African swine fever virus (ASFV) vaccine can induce efficient immune response, but the potential mechanism remains unsolved. In order to investigate the efficacy of recombinant viruses (VSV-p35, VSV-p72)-mediated dendritic cells (DCs) maturation and the...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/12/1/36 |
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| author | Yunyun Ma Junjun Shao Wei Liu Shandian Gao Guangqing Zhou Xuefeng Qi Huiyun Chang |
| author_facet | Yunyun Ma Junjun Shao Wei Liu Shandian Gao Guangqing Zhou Xuefeng Qi Huiyun Chang |
| author_sort | Yunyun Ma |
| collection | DOAJ |
| description | The vesicular stomatitis virus (VSV)-vectored African swine fever virus (ASFV) vaccine can induce efficient immune response, but the potential mechanism remains unsolved. In order to investigate the efficacy of recombinant viruses (VSV-p35, VSV-p72)-mediated dendritic cells (DCs) maturation and the mechanism of inducing T-cell immune response, the functional effects of recombinant viruses on DC activation and target antigens presentation were explored in this study. The results showed that surface-marked molecules (CD80, CD86, CD40, and MHC-II) and secreted cytokines (IL-4, TNF-α, IFN-γ) were highly expressed in the recombinant virus-infected DCs. In addition, the co-culture results of recombinant virus-treated DCs with naive T cells showed that the Th1- and Th17-type responses were effectively activated. Taken together, the study indicated that the VSV-vectored ASFV vaccine activated the maturation of DCs and the Th1- and Th17-type immune response, which provided a theoretical basis for the development of novel ASF vaccines. |
| format | Article |
| id | doaj-art-0c9b70139024499aba6a51ce3694f78f |
| institution | Kabale University |
| issn | 2306-7381 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj-art-0c9b70139024499aba6a51ce3694f78f2025-01-24T13:52:04ZengMDPI AGVeterinary Sciences2306-73812025-01-011213610.3390/vetsci12010036Molecular Mechanism of VSV-Vectored ASFV Vaccine Activating Immune Response in DCsYunyun Ma0Junjun Shao1Wei Liu2Shandian Gao3Guangqing Zhou4Xuefeng Qi5Huiyun Chang6State Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730030, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730030, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730030, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730030, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730030, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaState Key Laboratory for Animal Disease Control and Prevention, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730030, ChinaThe vesicular stomatitis virus (VSV)-vectored African swine fever virus (ASFV) vaccine can induce efficient immune response, but the potential mechanism remains unsolved. In order to investigate the efficacy of recombinant viruses (VSV-p35, VSV-p72)-mediated dendritic cells (DCs) maturation and the mechanism of inducing T-cell immune response, the functional effects of recombinant viruses on DC activation and target antigens presentation were explored in this study. The results showed that surface-marked molecules (CD80, CD86, CD40, and MHC-II) and secreted cytokines (IL-4, TNF-α, IFN-γ) were highly expressed in the recombinant virus-infected DCs. In addition, the co-culture results of recombinant virus-treated DCs with naive T cells showed that the Th1- and Th17-type responses were effectively activated. Taken together, the study indicated that the VSV-vectored ASFV vaccine activated the maturation of DCs and the Th1- and Th17-type immune response, which provided a theoretical basis for the development of novel ASF vaccines.https://www.mdpi.com/2306-7381/12/1/36dendritic cellsantigen presentationrecombinant virusesimmune responseASFV |
| spellingShingle | Yunyun Ma Junjun Shao Wei Liu Shandian Gao Guangqing Zhou Xuefeng Qi Huiyun Chang Molecular Mechanism of VSV-Vectored ASFV Vaccine Activating Immune Response in DCs Veterinary Sciences dendritic cells antigen presentation recombinant viruses immune response ASFV |
| title | Molecular Mechanism of VSV-Vectored ASFV Vaccine Activating Immune Response in DCs |
| title_full | Molecular Mechanism of VSV-Vectored ASFV Vaccine Activating Immune Response in DCs |
| title_fullStr | Molecular Mechanism of VSV-Vectored ASFV Vaccine Activating Immune Response in DCs |
| title_full_unstemmed | Molecular Mechanism of VSV-Vectored ASFV Vaccine Activating Immune Response in DCs |
| title_short | Molecular Mechanism of VSV-Vectored ASFV Vaccine Activating Immune Response in DCs |
| title_sort | molecular mechanism of vsv vectored asfv vaccine activating immune response in dcs |
| topic | dendritic cells antigen presentation recombinant viruses immune response ASFV |
| url | https://www.mdpi.com/2306-7381/12/1/36 |
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