Detection of Endocarditis Associated Pili Genes in Enterococcus Faecalis Clinical Isolates
Out of 207 specimens,118 Enterococcus faecalis isolates were obtained from different sources (urine, root canal, wound, vagina, and blood). The commonest sites of infections were the root canal (50.8%), followed by the urinary tract (38.1%), wound (8.4%), and the vagina (2.5%). Urine, root canal, a...
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University of Baghdad
2025-01-01
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| Series: | Ibn Al-Haitham Journal for Pure and Applied Sciences |
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| Online Access: | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3539 |
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| author | Ayat Ahmed Najm May Talib Flayyih |
| author_facet | Ayat Ahmed Najm May Talib Flayyih |
| author_sort | Ayat Ahmed Najm |
| collection | DOAJ |
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Out of 207 specimens,118 Enterococcus faecalis isolates were obtained from different sources (urine, root canal, wound, vagina, and blood). The commonest sites of infections were the root canal (50.8%), followed by the urinary tract (38.1%), wound (8.4%), and the vagina (2.5%). Urine, root canal, and wound samples from individuals aged 20–40 years old showed the highest prevalence of Enterococcus faecalis isolates, except for the vagina, where those over 40 years old recorded the highest rates of isolates. According to gender, females had the highest prevalence of Enterococcus faecalis isolates in urine, root canal, and vaginal samples, with the exception of the wound source, where males had a higher rate of isolates than females. The antibiotic susceptibility test results indicated that all Enterococcus isolates were resistant to these antibiotics. All Enterococcus isolates (100%) demonstrated resistance to Azithromycin, Clarithromycin, Erythromycin, Doxycycline, Minocycline, Tetracycline, and 64.7% to Gentamicin. On the other hand, 100% of the isolates showed susceptibility to Linezolid, Daptomycin, Teicoplanin, Tigecycline, and Vancomycin, while 58.8% showed susceptibility to Streptomycin, 76.4% to Ciprofloxacin, and 94.1% to Ampicillin. The PCR technique identified the presence of epbA and epbC genes in 28 Enterococcus faecalis isolates. The results of PCR showed that all of the isolates had genes epbA (111bp) and epbC (85bp).
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| format | Article |
| id | doaj-art-0c9a21fa293a46fb9911f915a3269061 |
| institution | Kabale University |
| issn | 1609-4042 2521-3407 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | University of Baghdad |
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| series | Ibn Al-Haitham Journal for Pure and Applied Sciences |
| spelling | doaj-art-0c9a21fa293a46fb9911f915a32690612025-01-22T01:21:08ZengUniversity of BaghdadIbn Al-Haitham Journal for Pure and Applied Sciences1609-40422521-34072025-01-0138110.30526/38.1.3539Detection of Endocarditis Associated Pili Genes in Enterococcus Faecalis Clinical IsolatesAyat Ahmed Najm0https://orcid.org/0009-0004-8398-7978May Talib Flayyih 1https://orcid.org/0000-0001-8213-4691Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq.Department of Biology, College of Sciences, University of Baghdad, Baghdad, Iraq. Out of 207 specimens,118 Enterococcus faecalis isolates were obtained from different sources (urine, root canal, wound, vagina, and blood). The commonest sites of infections were the root canal (50.8%), followed by the urinary tract (38.1%), wound (8.4%), and the vagina (2.5%). Urine, root canal, and wound samples from individuals aged 20–40 years old showed the highest prevalence of Enterococcus faecalis isolates, except for the vagina, where those over 40 years old recorded the highest rates of isolates. According to gender, females had the highest prevalence of Enterococcus faecalis isolates in urine, root canal, and vaginal samples, with the exception of the wound source, where males had a higher rate of isolates than females. The antibiotic susceptibility test results indicated that all Enterococcus isolates were resistant to these antibiotics. All Enterococcus isolates (100%) demonstrated resistance to Azithromycin, Clarithromycin, Erythromycin, Doxycycline, Minocycline, Tetracycline, and 64.7% to Gentamicin. On the other hand, 100% of the isolates showed susceptibility to Linezolid, Daptomycin, Teicoplanin, Tigecycline, and Vancomycin, while 58.8% showed susceptibility to Streptomycin, 76.4% to Ciprofloxacin, and 94.1% to Ampicillin. The PCR technique identified the presence of epbA and epbC genes in 28 Enterococcus faecalis isolates. The results of PCR showed that all of the isolates had genes epbA (111bp) and epbC (85bp). https://jih.uobaghdad.edu.iq/index.php/j/article/view/3539EndocarditisPili related to biofilmEnterococcus faecalisEbpAEbpBEbpC |
| spellingShingle | Ayat Ahmed Najm May Talib Flayyih Detection of Endocarditis Associated Pili Genes in Enterococcus Faecalis Clinical Isolates Ibn Al-Haitham Journal for Pure and Applied Sciences Endocarditis Pili related to biofilm Enterococcus faecalis EbpA EbpB EbpC |
| title | Detection of Endocarditis Associated Pili Genes in Enterococcus Faecalis Clinical Isolates |
| title_full | Detection of Endocarditis Associated Pili Genes in Enterococcus Faecalis Clinical Isolates |
| title_fullStr | Detection of Endocarditis Associated Pili Genes in Enterococcus Faecalis Clinical Isolates |
| title_full_unstemmed | Detection of Endocarditis Associated Pili Genes in Enterococcus Faecalis Clinical Isolates |
| title_short | Detection of Endocarditis Associated Pili Genes in Enterococcus Faecalis Clinical Isolates |
| title_sort | detection of endocarditis associated pili genes in enterococcus faecalis clinical isolates |
| topic | Endocarditis Pili related to biofilm Enterococcus faecalis EbpA EbpB EbpC |
| url | https://jih.uobaghdad.edu.iq/index.php/j/article/view/3539 |
| work_keys_str_mv | AT ayatahmednajm detectionofendocarditisassociatedpiligenesinenterococcusfaecalisclinicalisolates AT maytalibflayyih detectionofendocarditisassociatedpiligenesinenterococcusfaecalisclinicalisolates |