Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy
Stem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES) cells provide a great promise for cell replacement...
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| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/9405204 |
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| author | Xinxin Han Liming Yu Jie Ren Min Wang Zhongliang Liu Xinyu Hu Daiyu Hu Yihong Chen Li Chen Ying Zhang Yuehua Liu Xiaoqing Zhang Hua He Zhengliang Gao |
| author_facet | Xinxin Han Liming Yu Jie Ren Min Wang Zhongliang Liu Xinyu Hu Daiyu Hu Yihong Chen Li Chen Ying Zhang Yuehua Liu Xiaoqing Zhang Hua He Zhengliang Gao |
| author_sort | Xinxin Han |
| collection | DOAJ |
| description | Stem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES) cells provide a great promise for cell replacement therapies. Optimized protocols for neural differentiation are necessary to produce functional human neural stem cells (hNSCs) for cell therapy. However, the qualified procedure is scarce and detailed features of hNSCs originated from hES cells are still unclear. In this study, we developed a method to obtain hNSCs from hES cells, by which we could harvest abundant hNSCs in a relatively short time. Then, we examined the expression of pluripotent and multipotent marker genes through immunostaining and confirmed differentiation potential of the differentiated hNSCs. Furthermore, we analyzed the mitotic activity of these hNSCs. In this report, we provided comprehensive features of hNSCs and delivered the knowledge about how to obtain more high-quality hNSCs from hES cells which may help to accelerate the NSC-based therapies in brain injury treatment. |
| format | Article |
| id | doaj-art-0c9a078200b34a18943b659f6047daa0 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-0c9a078200b34a18943b659f6047daa02025-02-03T06:13:48ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/94052049405204Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell TherapyXinxin Han0Liming Yu1Jie Ren2Min Wang3Zhongliang Liu4Xinyu Hu5Daiyu Hu6Yihong Chen7Li Chen8Ying Zhang9Yuehua Liu10Xiaoqing Zhang11Hua He12Zhengliang Gao13Shanghai Stomatological Hospital, Fudan University, Shanghai 200001, ChinaShanghai Stomatological Hospital, Fudan University, Shanghai 200001, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaSchool of Medicine, Jiaxing University, Zhejiang 314001, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaChangzheng Hospital, Second Affiliated Hospital of Second Military Medical University, Shanghai 200003, ChinaShanghai Stomatological Hospital, Fudan University, Shanghai 200001, ChinaShanghai Stomatological Hospital, Fudan University, Shanghai 200001, ChinaShanghai Stomatological Hospital, Fudan University, Shanghai 200001, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Tongji University Advanced Institute of Translational Medicine, Shanghai 200092, ChinaChangzheng Hospital, Second Affiliated Hospital of Second Military Medical University, Shanghai 200003, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Tongji University Advanced Institute of Translational Medicine, Shanghai 200092, ChinaStem cell-based therapies have been used for repairing damaged brain tissue and helping functional recovery after brain injury. Aberrance neurogenesis is related with brain injury, and multipotential neural stem cells from human embryonic stem (hES) cells provide a great promise for cell replacement therapies. Optimized protocols for neural differentiation are necessary to produce functional human neural stem cells (hNSCs) for cell therapy. However, the qualified procedure is scarce and detailed features of hNSCs originated from hES cells are still unclear. In this study, we developed a method to obtain hNSCs from hES cells, by which we could harvest abundant hNSCs in a relatively short time. Then, we examined the expression of pluripotent and multipotent marker genes through immunostaining and confirmed differentiation potential of the differentiated hNSCs. Furthermore, we analyzed the mitotic activity of these hNSCs. In this report, we provided comprehensive features of hNSCs and delivered the knowledge about how to obtain more high-quality hNSCs from hES cells which may help to accelerate the NSC-based therapies in brain injury treatment.http://dx.doi.org/10.1155/2017/9405204 |
| spellingShingle | Xinxin Han Liming Yu Jie Ren Min Wang Zhongliang Liu Xinyu Hu Daiyu Hu Yihong Chen Li Chen Ying Zhang Yuehua Liu Xiaoqing Zhang Hua He Zhengliang Gao Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy Stem Cells International |
| title | Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy |
| title_full | Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy |
| title_fullStr | Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy |
| title_full_unstemmed | Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy |
| title_short | Efficient and Fast Differentiation of Human Neural Stem Cells from Human Embryonic Stem Cells for Cell Therapy |
| title_sort | efficient and fast differentiation of human neural stem cells from human embryonic stem cells for cell therapy |
| url | http://dx.doi.org/10.1155/2017/9405204 |
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