Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids.
The cytochrome P450 CYP168A1 from Pseudomonas aeruginosa was cloned and expressed in Escherichia coli followed by purification and characterization of function. CYP168A1 is a fatty acid hydroxylase that hydroxylates saturated fatty acids, including myristic (0.30 min-1), palmitic (1.61 min-1) and st...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0265227&type=printable |
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| author | Claire L Price Andrew G S Warrilow Nicola J Rolley Josie E Parker Vera Thoss Diane E Kelly Nicolae Corcionivoschi Steven L Kelly |
| author_facet | Claire L Price Andrew G S Warrilow Nicola J Rolley Josie E Parker Vera Thoss Diane E Kelly Nicolae Corcionivoschi Steven L Kelly |
| author_sort | Claire L Price |
| collection | DOAJ |
| description | The cytochrome P450 CYP168A1 from Pseudomonas aeruginosa was cloned and expressed in Escherichia coli followed by purification and characterization of function. CYP168A1 is a fatty acid hydroxylase that hydroxylates saturated fatty acids, including myristic (0.30 min-1), palmitic (1.61 min-1) and stearic acids (1.24 min-1), at both the ω-1- and ω-2-positions. However, CYP168A1 only hydroxylates unsaturated fatty acids, including palmitoleic (0.38 min-1), oleic (1.28 min-1) and linoleic acids (0.35 min-1), at the ω-1-position. CYP168A1 exhibited a catalytic preference for palmitic, oleic and stearic acids as substrates in keeping with the phosphatidylcholine-rich environment deep in the lung that is colonized by P. aeruginosa. |
| format | Article |
| id | doaj-art-0c90bb703fce45e4b14eb43700b54656 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-0c90bb703fce45e4b14eb43700b546562025-08-20T02:02:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01173e026522710.1371/journal.pone.0265227Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids.Claire L PriceAndrew G S WarrilowNicola J RolleyJosie E ParkerVera ThossDiane E KellyNicolae CorcionivoschiSteven L KellyThe cytochrome P450 CYP168A1 from Pseudomonas aeruginosa was cloned and expressed in Escherichia coli followed by purification and characterization of function. CYP168A1 is a fatty acid hydroxylase that hydroxylates saturated fatty acids, including myristic (0.30 min-1), palmitic (1.61 min-1) and stearic acids (1.24 min-1), at both the ω-1- and ω-2-positions. However, CYP168A1 only hydroxylates unsaturated fatty acids, including palmitoleic (0.38 min-1), oleic (1.28 min-1) and linoleic acids (0.35 min-1), at the ω-1-position. CYP168A1 exhibited a catalytic preference for palmitic, oleic and stearic acids as substrates in keeping with the phosphatidylcholine-rich environment deep in the lung that is colonized by P. aeruginosa.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0265227&type=printable |
| spellingShingle | Claire L Price Andrew G S Warrilow Nicola J Rolley Josie E Parker Vera Thoss Diane E Kelly Nicolae Corcionivoschi Steven L Kelly Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids. PLoS ONE |
| title | Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids. |
| title_full | Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids. |
| title_fullStr | Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids. |
| title_full_unstemmed | Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids. |
| title_short | Cytochrome P450 168A1 from Pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids. |
| title_sort | cytochrome p450 168a1 from pseudomonas aeruginosa is involved in the hydroxylation of biologically relevant fatty acids |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0265227&type=printable |
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