Black Phosphorous-Based Surface Plasmon Resonance Biosensor for Malaria Diagnosis

This study presents a black phosphorus-based surface plasmon resonance (SPR) biosensor for malaria detection, integrating silicon nitride (Si<sub>3</sub>N<sub>4</sub>) and single-stranded DNA (ssDNA) to enhance sensitivity and molecular recognition. The biosensor configuratio...

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Main Authors: Talia Tene, Yesenia Cevallos, Paola Gabriela Vinueza-Naranjo, Deysi Inca, Cristian Vacacela Gomez
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Sensors
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Online Access:https://www.mdpi.com/1424-8220/25/7/2068
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author Talia Tene
Yesenia Cevallos
Paola Gabriela Vinueza-Naranjo
Deysi Inca
Cristian Vacacela Gomez
author_facet Talia Tene
Yesenia Cevallos
Paola Gabriela Vinueza-Naranjo
Deysi Inca
Cristian Vacacela Gomez
author_sort Talia Tene
collection DOAJ
description This study presents a black phosphorus-based surface plasmon resonance (SPR) biosensor for malaria detection, integrating silicon nitride (Si<sub>3</sub>N<sub>4</sub>) and single-stranded DNA (ssDNA) to enhance sensitivity and molecular recognition. The biosensor configurations were optimized through numerical simulations, evaluating metal thickness, dielectric layer thickness, and the number of black phosphorus layers to achieve maximum performance. The optimized system (Opt-Sys<sub>4</sub>) exhibited high sensitivity (464.4°/RIU for early-stage malaria) and improved detection accuracy, outperforming conventional SPR sensors. Performance was assessed across malaria progression stages, demonstrating a clear resonance shift, increased attenuation, and enhanced biomolecular interactions. Key metrics, including the figure of merit, limit of detection, and comprehensive sensitivity factor, confirmed the sensor’s superior performance. Comparative analysis against state-of-the-art SPR biosensors further validated their capability for highly sensitive and specific malaria detection. These findings establish a promising plasmonic biosensing platform for early malaria diagnosis, potentially improving disease management in resource-limited settings.
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series Sensors
spelling doaj-art-0c8e095075a941c48f305ad14dc043ed2025-08-20T02:15:46ZengMDPI AGSensors1424-82202025-03-01257206810.3390/s25072068Black Phosphorous-Based Surface Plasmon Resonance Biosensor for Malaria DiagnosisTalia Tene0Yesenia Cevallos1Paola Gabriela Vinueza-Naranjo2Deysi Inca3Cristian Vacacela Gomez4Department of Chemistry, Universidad Técnica Particular de Loja, Loja 110160, EcuadorUniversidad San Francisco de Quito IMNE, Diego de Robles s/n, Cumbayá, Quito 170901, EcuadorCollege of Engineering, Universidad Nacional de Chimborazo, Riobamba 060108, EcuadorCollege of Engineering, Universidad Nacional de Chimborazo, Riobamba 060108, EcuadorINFN-Laboratori Nazionali di Frascati, Via E. Fermi 54, 00044 Frascati, ItalyThis study presents a black phosphorus-based surface plasmon resonance (SPR) biosensor for malaria detection, integrating silicon nitride (Si<sub>3</sub>N<sub>4</sub>) and single-stranded DNA (ssDNA) to enhance sensitivity and molecular recognition. The biosensor configurations were optimized through numerical simulations, evaluating metal thickness, dielectric layer thickness, and the number of black phosphorus layers to achieve maximum performance. The optimized system (Opt-Sys<sub>4</sub>) exhibited high sensitivity (464.4°/RIU for early-stage malaria) and improved detection accuracy, outperforming conventional SPR sensors. Performance was assessed across malaria progression stages, demonstrating a clear resonance shift, increased attenuation, and enhanced biomolecular interactions. Key metrics, including the figure of merit, limit of detection, and comprehensive sensitivity factor, confirmed the sensor’s superior performance. Comparative analysis against state-of-the-art SPR biosensors further validated their capability for highly sensitive and specific malaria detection. These findings establish a promising plasmonic biosensing platform for early malaria diagnosis, potentially improving disease management in resource-limited settings.https://www.mdpi.com/1424-8220/25/7/2068surface plasmon resonanceKretschmann configurationtransfer matrix methodsilicon nitrideblack phosphorousbiosensors
spellingShingle Talia Tene
Yesenia Cevallos
Paola Gabriela Vinueza-Naranjo
Deysi Inca
Cristian Vacacela Gomez
Black Phosphorous-Based Surface Plasmon Resonance Biosensor for Malaria Diagnosis
Sensors
surface plasmon resonance
Kretschmann configuration
transfer matrix method
silicon nitride
black phosphorous
biosensors
title Black Phosphorous-Based Surface Plasmon Resonance Biosensor for Malaria Diagnosis
title_full Black Phosphorous-Based Surface Plasmon Resonance Biosensor for Malaria Diagnosis
title_fullStr Black Phosphorous-Based Surface Plasmon Resonance Biosensor for Malaria Diagnosis
title_full_unstemmed Black Phosphorous-Based Surface Plasmon Resonance Biosensor for Malaria Diagnosis
title_short Black Phosphorous-Based Surface Plasmon Resonance Biosensor for Malaria Diagnosis
title_sort black phosphorous based surface plasmon resonance biosensor for malaria diagnosis
topic surface plasmon resonance
Kretschmann configuration
transfer matrix method
silicon nitride
black phosphorous
biosensors
url https://www.mdpi.com/1424-8220/25/7/2068
work_keys_str_mv AT taliatene blackphosphorousbasedsurfaceplasmonresonancebiosensorformalariadiagnosis
AT yeseniacevallos blackphosphorousbasedsurfaceplasmonresonancebiosensorformalariadiagnosis
AT paolagabrielavinuezanaranjo blackphosphorousbasedsurfaceplasmonresonancebiosensorformalariadiagnosis
AT deysiinca blackphosphorousbasedsurfaceplasmonresonancebiosensorformalariadiagnosis
AT cristianvacacelagomez blackphosphorousbasedsurfaceplasmonresonancebiosensorformalariadiagnosis