Anthocyanin attenuates disturbance of intestinal barrier in high fat-high cholesterol diet-challenged mice through regulating the response of T helper 17 cells
Anthocyanin, as a typical food bioactive molecule, is capable of reversing inflammatory, oxidative and allergic condition thus contributes to intestinal health. We were wondering whether anthocyanin has influence on the infiltration of inflammatory cells into the intestinal mucosa and thus help enha...
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Tsinghua University Press
2025-01-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2024.9250024 |
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| author | Qiannan Liu Juan Pang Yi Tang Yiran You Jiaxin Mi Jinghe Xiao Yu Chen Wenhua Ling |
| author_facet | Qiannan Liu Juan Pang Yi Tang Yiran You Jiaxin Mi Jinghe Xiao Yu Chen Wenhua Ling |
| author_sort | Qiannan Liu |
| collection | DOAJ |
| description | Anthocyanin, as a typical food bioactive molecule, is capable of reversing inflammatory, oxidative and allergic condition thus contributes to intestinal health. We were wondering whether anthocyanin has influence on the infiltration of inflammatory cells into the intestinal mucosa and thus help enhancing intestinal barrier which could be damaged in some metabolic diseases. In this study, the influence of anthocyanin (administered orally) on the alterations (including structure and permeability) of the intestinal mucosa in mice in response to a high fat-high cholesterol (HFHC) diet was investigated. Primary T helper 17 (Th17) cells were isolated from mouse intestine tissues to observe the modulatory role of anthocyanin through the transcription phosphorylated STAT3 (p-STAT3). The results indicated that anthocyanin significantly alleviated HFHC-induced impairment in the intestinal structures and permeability in a dose-dependent manner; moreover, anthocyanin appeared to inhibit HFHC induced the expression of p-STAT3, thereby disturbing Th17 cell differentiation. In high-fat diet (HFD, cholesterol level non-modified)-challenged mice selective p-STAT3 inhibitor significantly reversed the effects of anthocyanin, which were decreased amount of interleukin (IL)-17A (produced and released from Th17 cells) and the protected intestinal structure/function. In summary, the results of this study suggest that anthocyanin may attenuate the damage of intestinal barrier in HFHC mice through regulating intestinal STAT3-Th17-IL-17A signal transduction pathway. |
| format | Article |
| id | doaj-art-0c8d3fbd7ec84608b21f2212baf0681c |
| institution | DOAJ |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-0c8d3fbd7ec84608b21f2212baf0681c2025-08-20T02:47:52ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302025-01-01141925002410.26599/FSHW.2024.9250024Anthocyanin attenuates disturbance of intestinal barrier in high fat-high cholesterol diet-challenged mice through regulating the response of T helper 17 cellsQiannan Liu0Juan Pang1Yi Tang2Yiran You3Jiaxin Mi4Jinghe Xiao5Yu Chen6Wenhua Ling7Department of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510000, ChinaLaboratory of Clinical Pharmacy and Adverse Drug Reaction, West China Hospital, Sichuan University, Chengdu 610000, ChinaDepartment of Nutrition, The First People's Hospital of Zhaoqing, Zhaoqing 526000, ChinaDepartment of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510000, ChinaDepartment of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510000, ChinaDepartment of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510000, ChinaDepartment of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510000, ChinaDepartment of Nutrition, School of Public Health, Sun Yat-sen University, Guangzhou 510000, ChinaAnthocyanin, as a typical food bioactive molecule, is capable of reversing inflammatory, oxidative and allergic condition thus contributes to intestinal health. We were wondering whether anthocyanin has influence on the infiltration of inflammatory cells into the intestinal mucosa and thus help enhancing intestinal barrier which could be damaged in some metabolic diseases. In this study, the influence of anthocyanin (administered orally) on the alterations (including structure and permeability) of the intestinal mucosa in mice in response to a high fat-high cholesterol (HFHC) diet was investigated. Primary T helper 17 (Th17) cells were isolated from mouse intestine tissues to observe the modulatory role of anthocyanin through the transcription phosphorylated STAT3 (p-STAT3). The results indicated that anthocyanin significantly alleviated HFHC-induced impairment in the intestinal structures and permeability in a dose-dependent manner; moreover, anthocyanin appeared to inhibit HFHC induced the expression of p-STAT3, thereby disturbing Th17 cell differentiation. In high-fat diet (HFD, cholesterol level non-modified)-challenged mice selective p-STAT3 inhibitor significantly reversed the effects of anthocyanin, which were decreased amount of interleukin (IL)-17A (produced and released from Th17 cells) and the protected intestinal structure/function. In summary, the results of this study suggest that anthocyanin may attenuate the damage of intestinal barrier in HFHC mice through regulating intestinal STAT3-Th17-IL-17A signal transduction pathway.https://www.sciopen.com/article/10.26599/FSHW.2024.9250024anthocyaninintestinal barrier dysfunctionstat3t helper 17 (th17) cellinterleukin (il)-17a |
| spellingShingle | Qiannan Liu Juan Pang Yi Tang Yiran You Jiaxin Mi Jinghe Xiao Yu Chen Wenhua Ling Anthocyanin attenuates disturbance of intestinal barrier in high fat-high cholesterol diet-challenged mice through regulating the response of T helper 17 cells Food Science and Human Wellness anthocyanin intestinal barrier dysfunction stat3 t helper 17 (th17) cell interleukin (il)-17a |
| title | Anthocyanin attenuates disturbance of intestinal barrier in high fat-high cholesterol diet-challenged mice through regulating the response of T helper 17 cells |
| title_full | Anthocyanin attenuates disturbance of intestinal barrier in high fat-high cholesterol diet-challenged mice through regulating the response of T helper 17 cells |
| title_fullStr | Anthocyanin attenuates disturbance of intestinal barrier in high fat-high cholesterol diet-challenged mice through regulating the response of T helper 17 cells |
| title_full_unstemmed | Anthocyanin attenuates disturbance of intestinal barrier in high fat-high cholesterol diet-challenged mice through regulating the response of T helper 17 cells |
| title_short | Anthocyanin attenuates disturbance of intestinal barrier in high fat-high cholesterol diet-challenged mice through regulating the response of T helper 17 cells |
| title_sort | anthocyanin attenuates disturbance of intestinal barrier in high fat high cholesterol diet challenged mice through regulating the response of t helper 17 cells |
| topic | anthocyanin intestinal barrier dysfunction stat3 t helper 17 (th17) cell interleukin (il)-17a |
| url | https://www.sciopen.com/article/10.26599/FSHW.2024.9250024 |
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