Early Prediction of Nephropathy in Iraqi Patient with Diabetes Type II by Evaluating Some Relevant Biochemical Factors

    Type 2 diabetes mellitus (T2DM) is a complex disease, which affects many organs besides the pancreas such as the kidney, liver, brain and eye. Due to hyperglycemia at long periods and uncontrolled on diabetes with presence of other risk factors, diabetes complications could occurr. Diabetes com...

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Main Authors: Reham Khaldoon Ibrahim, Kadhim K. Ghudhaib, Ali Abdulmajid Dyab Allawi
Format: Article
Language:English
Published: University of Baghdad, College of Science for Women 2024-05-01
Series:مجلة بغداد للعلوم
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Online Access:https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8008
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author Reham Khaldoon Ibrahim
Kadhim K. Ghudhaib
Ali Abdulmajid Dyab Allawi
author_facet Reham Khaldoon Ibrahim
Kadhim K. Ghudhaib
Ali Abdulmajid Dyab Allawi
author_sort Reham Khaldoon Ibrahim
collection DOAJ
description     Type 2 diabetes mellitus (T2DM) is a complex disease, which affects many organs besides the pancreas such as the kidney, liver, brain and eye. Due to hyperglycemia at long periods and uncontrolled on diabetes with presence of other risk factors, diabetes complications could occurr. Diabetes complications include microvascular and macrovascular complications that target the kidneys. The aim of this study is to predict early fibrosis of the renal glomeruli and tubules by evaluating the levels of angiotensin-converting enzyme -2(ACE-2), connective tissue growth factor (CTGF) and some relevant biochemical factors in patients with type 2 diabetes. The study included 120 male and female ranging in age 30-65 years old, they were subdivided into three groups according to ACR criteria include normoalbuminuria, microalbuminuria, macroalbuminuria (30 patients for each group) and 30 healthy people served as the control group, who visited Baghdad Teaching Hospital / Medical City and Al-Yarmouk Teaching Hospital, at the period between December 2021 and May 2022.  Lipid profile, FBS, urea levels were estimated using calorimetric techniques. ACE-2, CTGF levels were determined using the ELISA technique. The results showed significant differences between groups of patients and control group for (CTGF), (ACE-2) levels were found to be significant increase in patients’ groups than healthy control. Also, the results showed that both fasting blood sugar (FBS) and hemoglobin A1c (HbA1C) were significantly increased in patients’ groups compared to healthy group. Furthermore, estimated glomerular filtration rate (eGFR) revealed high significant differences among all the studied groups, as well as   albumin to creatinine ratio (ACR) which showed high significant differences among the three patients’ groups  which represents the basic criteria for classification of patient groups. On the basis of the obtained results in this study, it can be concluded that each of ACE-2 and CTGF markers can be applied as early reliable prognosticated factors for detection disease progression.
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spelling doaj-art-0c897a205afa49e2abb10a2d4afaf0c12025-08-20T03:15:47ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862024-05-0121510.21123/bsj.2023.8008Early Prediction of Nephropathy in Iraqi Patient with Diabetes Type II by Evaluating Some Relevant Biochemical FactorsReham Khaldoon Ibrahim0https://orcid.org/0000-0001-8472-3502Kadhim K. Ghudhaib1https://orcid.org/0000-0003-4966-4070Ali Abdulmajid Dyab Allawi2https://orcid.org/0000-0002-1282-1813Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq.Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq.Department of Medicine, College of Medicine, University of Baghdad, Baghdad, Iraq.     Type 2 diabetes mellitus (T2DM) is a complex disease, which affects many organs besides the pancreas such as the kidney, liver, brain and eye. Due to hyperglycemia at long periods and uncontrolled on diabetes with presence of other risk factors, diabetes complications could occurr. Diabetes complications include microvascular and macrovascular complications that target the kidneys. The aim of this study is to predict early fibrosis of the renal glomeruli and tubules by evaluating the levels of angiotensin-converting enzyme -2(ACE-2), connective tissue growth factor (CTGF) and some relevant biochemical factors in patients with type 2 diabetes. The study included 120 male and female ranging in age 30-65 years old, they were subdivided into three groups according to ACR criteria include normoalbuminuria, microalbuminuria, macroalbuminuria (30 patients for each group) and 30 healthy people served as the control group, who visited Baghdad Teaching Hospital / Medical City and Al-Yarmouk Teaching Hospital, at the period between December 2021 and May 2022.  Lipid profile, FBS, urea levels were estimated using calorimetric techniques. ACE-2, CTGF levels were determined using the ELISA technique. The results showed significant differences between groups of patients and control group for (CTGF), (ACE-2) levels were found to be significant increase in patients’ groups than healthy control. Also, the results showed that both fasting blood sugar (FBS) and hemoglobin A1c (HbA1C) were significantly increased in patients’ groups compared to healthy group. Furthermore, estimated glomerular filtration rate (eGFR) revealed high significant differences among all the studied groups, as well as   albumin to creatinine ratio (ACR) which showed high significant differences among the three patients’ groups  which represents the basic criteria for classification of patient groups. On the basis of the obtained results in this study, it can be concluded that each of ACE-2 and CTGF markers can be applied as early reliable prognosticated factors for detection disease progression. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8008Angiotensin converting enzyme-2, albumin to creatinine ratio, connective tissue growth factor, Diabetes Mellitus. Diabetic Nephropathy
spellingShingle Reham Khaldoon Ibrahim
Kadhim K. Ghudhaib
Ali Abdulmajid Dyab Allawi
Early Prediction of Nephropathy in Iraqi Patient with Diabetes Type II by Evaluating Some Relevant Biochemical Factors
مجلة بغداد للعلوم
Angiotensin converting enzyme-2, albumin to creatinine ratio, connective tissue growth factor, Diabetes Mellitus. Diabetic Nephropathy
title Early Prediction of Nephropathy in Iraqi Patient with Diabetes Type II by Evaluating Some Relevant Biochemical Factors
title_full Early Prediction of Nephropathy in Iraqi Patient with Diabetes Type II by Evaluating Some Relevant Biochemical Factors
title_fullStr Early Prediction of Nephropathy in Iraqi Patient with Diabetes Type II by Evaluating Some Relevant Biochemical Factors
title_full_unstemmed Early Prediction of Nephropathy in Iraqi Patient with Diabetes Type II by Evaluating Some Relevant Biochemical Factors
title_short Early Prediction of Nephropathy in Iraqi Patient with Diabetes Type II by Evaluating Some Relevant Biochemical Factors
title_sort early prediction of nephropathy in iraqi patient with diabetes type ii by evaluating some relevant biochemical factors
topic Angiotensin converting enzyme-2, albumin to creatinine ratio, connective tissue growth factor, Diabetes Mellitus. Diabetic Nephropathy
url https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8008
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