Screening key genes and miRNAs in early-stage colon adenocarcinoma by RNA-sequencing
Colon adenocarcinoma is the third leading cause of cancer-related deaths across the world, developing novel and non-invasive diagnostic and prognostic biomarkers for the early-stage colon adenocarcinoma at molecular level is essential. In our study, RNA-sequencing was performed to identify the diffe...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SAGE Publishing
2017-07-01
|
| Series: | Tumor Biology |
| Online Access: | https://doi.org/10.1177/1010428317714899 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849416440718819328 |
|---|---|
| author | Jixi Liu Fang Liu Xiaoou Li Xin Song Lei Zhou Jianzheng Jie |
| author_facet | Jixi Liu Fang Liu Xiaoou Li Xin Song Lei Zhou Jianzheng Jie |
| author_sort | Jixi Liu |
| collection | DOAJ |
| description | Colon adenocarcinoma is the third leading cause of cancer-related deaths across the world, developing novel and non-invasive diagnostic and prognostic biomarkers for the early-stage colon adenocarcinoma at molecular level is essential. In our study, RNA-sequencing was performed to identify the differentially expressed genes and miRNAs (DEmiRNAs) in early-stage colon adenocarcinoma compared to tissues of precancerous lesions, colonic intraepithelial neoplasia. The DEmiRNA-target interaction network was constructed and functional annotation of targets of DEmiRNAs was performed. The Cancer Genome Atlas was used to verify the expression of selected differentially expressed genes. The receiver operating characteristic analyses of selected differentially expressed genes was performed. In total, 865 differentially expressed genes, 26 DEmiRNAs, and 329 DEmiRNA-target pairs were obtained. Based on the early-stage colon adenocarcinoma network, miR-548c-5p, miR-548i, and miR-548am-5p were the top three DEmiRNAs that covered most differentially expressed genes. NTRK2, DTNA, and BTG2 were the top three differentially expressed genes regulated by most DEmiRNAs. Cancer and colorectal cancer pathways were two significantly enriched pathways in early-stage colon adenocarcinoma. The common differentially expressed genes in both the pathways were AXIN2, Smad2, Smad4, PIK3R1, and BCL2. The expression levels of eight differentially expressed genes (NTRK2, DTNA, BTG2, COL11A1, Smad2, Smad4, PIK3R1, and BCL2) in The Cancer Genome Atlas database were compatible with our RNA-sequencing. All these eight differentially expressed genes and AXIN2 had the potential diagnosis value for Colon adenocarcinoma. In conclusion, a total of ten differentially expressed genes (NTRK2, DTNA, BTG2, COLCA1, COL11A1, AXIN2, Smad2, Smad4, PIK3R1, and BCL2) and four DEmiRNAs (miR-548c-5p, miR-548i, mir-424-5p, and miR-548am-5p) may be involved in the pathogenesis of early-stage colon adenocarcinoma which may make a contribution for developing new diagnostic and therapeutic strategies for early-stage colon adenocarcinoma. |
| format | Article |
| id | doaj-art-0c5faa15e95e4dbcbe5402d1c7a5e604 |
| institution | Kabale University |
| issn | 1423-0380 |
| language | English |
| publishDate | 2017-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Tumor Biology |
| spelling | doaj-art-0c5faa15e95e4dbcbe5402d1c7a5e6042025-08-20T03:33:11ZengSAGE PublishingTumor Biology1423-03802017-07-013910.1177/1010428317714899Screening key genes and miRNAs in early-stage colon adenocarcinoma by RNA-sequencingJixi LiuFang LiuXiaoou LiXin SongLei ZhouJianzheng JieColon adenocarcinoma is the third leading cause of cancer-related deaths across the world, developing novel and non-invasive diagnostic and prognostic biomarkers for the early-stage colon adenocarcinoma at molecular level is essential. In our study, RNA-sequencing was performed to identify the differentially expressed genes and miRNAs (DEmiRNAs) in early-stage colon adenocarcinoma compared to tissues of precancerous lesions, colonic intraepithelial neoplasia. The DEmiRNA-target interaction network was constructed and functional annotation of targets of DEmiRNAs was performed. The Cancer Genome Atlas was used to verify the expression of selected differentially expressed genes. The receiver operating characteristic analyses of selected differentially expressed genes was performed. In total, 865 differentially expressed genes, 26 DEmiRNAs, and 329 DEmiRNA-target pairs were obtained. Based on the early-stage colon adenocarcinoma network, miR-548c-5p, miR-548i, and miR-548am-5p were the top three DEmiRNAs that covered most differentially expressed genes. NTRK2, DTNA, and BTG2 were the top three differentially expressed genes regulated by most DEmiRNAs. Cancer and colorectal cancer pathways were two significantly enriched pathways in early-stage colon adenocarcinoma. The common differentially expressed genes in both the pathways were AXIN2, Smad2, Smad4, PIK3R1, and BCL2. The expression levels of eight differentially expressed genes (NTRK2, DTNA, BTG2, COL11A1, Smad2, Smad4, PIK3R1, and BCL2) in The Cancer Genome Atlas database were compatible with our RNA-sequencing. All these eight differentially expressed genes and AXIN2 had the potential diagnosis value for Colon adenocarcinoma. In conclusion, a total of ten differentially expressed genes (NTRK2, DTNA, BTG2, COLCA1, COL11A1, AXIN2, Smad2, Smad4, PIK3R1, and BCL2) and four DEmiRNAs (miR-548c-5p, miR-548i, mir-424-5p, and miR-548am-5p) may be involved in the pathogenesis of early-stage colon adenocarcinoma which may make a contribution for developing new diagnostic and therapeutic strategies for early-stage colon adenocarcinoma.https://doi.org/10.1177/1010428317714899 |
| spellingShingle | Jixi Liu Fang Liu Xiaoou Li Xin Song Lei Zhou Jianzheng Jie Screening key genes and miRNAs in early-stage colon adenocarcinoma by RNA-sequencing Tumor Biology |
| title | Screening key genes and miRNAs in early-stage colon adenocarcinoma by RNA-sequencing |
| title_full | Screening key genes and miRNAs in early-stage colon adenocarcinoma by RNA-sequencing |
| title_fullStr | Screening key genes and miRNAs in early-stage colon adenocarcinoma by RNA-sequencing |
| title_full_unstemmed | Screening key genes and miRNAs in early-stage colon adenocarcinoma by RNA-sequencing |
| title_short | Screening key genes and miRNAs in early-stage colon adenocarcinoma by RNA-sequencing |
| title_sort | screening key genes and mirnas in early stage colon adenocarcinoma by rna sequencing |
| url | https://doi.org/10.1177/1010428317714899 |
| work_keys_str_mv | AT jixiliu screeningkeygenesandmirnasinearlystagecolonadenocarcinomabyrnasequencing AT fangliu screeningkeygenesandmirnasinearlystagecolonadenocarcinomabyrnasequencing AT xiaoouli screeningkeygenesandmirnasinearlystagecolonadenocarcinomabyrnasequencing AT xinsong screeningkeygenesandmirnasinearlystagecolonadenocarcinomabyrnasequencing AT leizhou screeningkeygenesandmirnasinearlystagecolonadenocarcinomabyrnasequencing AT jianzhengjie screeningkeygenesandmirnasinearlystagecolonadenocarcinomabyrnasequencing |