Unaltered maximal power and submaximal performance correlates with an oxidative vastus lateralis proteome phenotype during tapering in male cyclists

Abstract Little is known on how a short‐term reduction of training volume changes muscle proteome and physiological parameters. We investigated the impact of halving training volume during regular training of cyclists on physiological parameters in relation to vastus lateralis protein profiles and f...

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Main Authors: Pieter deLange, Giuseppe Petito, Hannah L. Notbohm, Antonia Giacco, Giovanni Renzone, Elena Silvestri, Arianna Cuomo, Frank Suhr, Thorsten Schiffer, Jonas Zacher, Federica Cioffi, Rosalba Senese, Andrea Scaloni, Moritz Schumann, Wilhelm Bloch
Format: Article
Language:English
Published: Wiley 2025-04-01
Series:Physiological Reports
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Online Access:https://doi.org/10.14814/phy2.70302
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author Pieter deLange
Giuseppe Petito
Hannah L. Notbohm
Antonia Giacco
Giovanni Renzone
Elena Silvestri
Arianna Cuomo
Frank Suhr
Thorsten Schiffer
Jonas Zacher
Federica Cioffi
Rosalba Senese
Andrea Scaloni
Moritz Schumann
Wilhelm Bloch
author_facet Pieter deLange
Giuseppe Petito
Hannah L. Notbohm
Antonia Giacco
Giovanni Renzone
Elena Silvestri
Arianna Cuomo
Frank Suhr
Thorsten Schiffer
Jonas Zacher
Federica Cioffi
Rosalba Senese
Andrea Scaloni
Moritz Schumann
Wilhelm Bloch
author_sort Pieter deLange
collection DOAJ
description Abstract Little is known on how a short‐term reduction of training volume changes muscle proteome and physiological parameters. We investigated the impact of halving training volume during regular training of cyclists on physiological parameters in relation to vastus lateralis protein profiles and fiber percentage ratios. Fifteen male cyclists (age: 30.1 ± 9.6 yrs.; VO2max: 59.4 ± 4.4 mL∙kg−1∙min−1; weekly training volume: 8.7 ± 2.3 h) participated in an 11‐week training intervention. During 2 weeks after a shared training programme for 9 weeks, a control group continued training and a taper group reduced training volume by 50%. No end‐point differences were found for peak power output, maximal oxygen uptake, or peak and mean power in a sprint test (p > 0.05), although in the taper group, muscle proteins involved in mitochondrial aerobic respiration increased whereas those involved in translation, protein catabolism, and actin organization decreased, without between‐group differences in type I/type II fiber percentage ratios. Tapering did not decrease power at the first (LT1) and second lactate threshold (LT2) compared to t0, whereas power increased in the control group (LT1: 216 ± 28 W vs. 238 ± 11 W, p = 0.042, LT2: 290 ± 42 W vs. 318 ± 13 W, p = 0.005). Our data indicate that transient 50% training volume reductions may be beneficial for oxidative metabolism in muscle.
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spelling doaj-art-0c59285de55c4be09a9c4a52abd67dc02025-08-20T03:53:23ZengWileyPhysiological Reports2051-817X2025-04-01138n/an/a10.14814/phy2.70302Unaltered maximal power and submaximal performance correlates with an oxidative vastus lateralis proteome phenotype during tapering in male cyclistsPieter deLange0Giuseppe Petito1Hannah L. Notbohm2Antonia Giacco3Giovanni Renzone4Elena Silvestri5Arianna Cuomo6Frank Suhr7Thorsten Schiffer8Jonas Zacher9Federica Cioffi10Rosalba Senese11Andrea Scaloni12Moritz Schumann13Wilhelm Bloch14Department of Environmental, Biological, and Pharmaceutical Sciences and Technologies University of Campania “Luigi Vanvitelli” Caserta ItalyDepartment of Environmental, Biological, and Pharmaceutical Sciences and Technologies University of Campania “Luigi Vanvitelli” Caserta ItalyDepartment of Molecular and Cellular Sport Medicine German Sports University Cologne GermanyDepartment of Sciences and Technologies University of Sannio Benevento ItalyProteomics, Metabolomics and Mass Spectrometry Laboratory ISPAAM, National Research Council Portici ItalyDepartment of Sciences and Technologies University of Sannio Benevento ItalyDepartment of Environmental, Biological, and Pharmaceutical Sciences and Technologies University of Campania “Luigi Vanvitelli” Caserta ItalyDivision of Molecular Exercise Physiology, Faculty of Life Sciences: Food, Nutrition and Health University of Bayreuth Kulmbach GermanyOutpatient Clinic for Sports Traumatology and Public Health Consultation German Sports University Cologne GermanyDepartment of Preventative and Rehabilitative Sports and Performance Medicine, Institute of Cardiovascular Research and Sports Medicine German Sports University Cologne GermanyDepartment of Sciences and Technologies University of Sannio Benevento ItalyDepartment of Environmental, Biological, and Pharmaceutical Sciences and Technologies University of Campania “Luigi Vanvitelli” Caserta ItalyProteomics, Metabolomics and Mass Spectrometry Laboratory ISPAAM, National Research Council Portici ItalyDepartment of Sports Medicine and Exercise Therapy Chemnitz University of Technology Chemnitz GermanyDepartment of Molecular and Cellular Sport Medicine German Sports University Cologne GermanyAbstract Little is known on how a short‐term reduction of training volume changes muscle proteome and physiological parameters. We investigated the impact of halving training volume during regular training of cyclists on physiological parameters in relation to vastus lateralis protein profiles and fiber percentage ratios. Fifteen male cyclists (age: 30.1 ± 9.6 yrs.; VO2max: 59.4 ± 4.4 mL∙kg−1∙min−1; weekly training volume: 8.7 ± 2.3 h) participated in an 11‐week training intervention. During 2 weeks after a shared training programme for 9 weeks, a control group continued training and a taper group reduced training volume by 50%. No end‐point differences were found for peak power output, maximal oxygen uptake, or peak and mean power in a sprint test (p > 0.05), although in the taper group, muscle proteins involved in mitochondrial aerobic respiration increased whereas those involved in translation, protein catabolism, and actin organization decreased, without between‐group differences in type I/type II fiber percentage ratios. Tapering did not decrease power at the first (LT1) and second lactate threshold (LT2) compared to t0, whereas power increased in the control group (LT1: 216 ± 28 W vs. 238 ± 11 W, p = 0.042, LT2: 290 ± 42 W vs. 318 ± 13 W, p = 0.005). Our data indicate that transient 50% training volume reductions may be beneficial for oxidative metabolism in muscle.https://doi.org/10.14814/phy2.70302aerobic capacityfiber percentage ratioprotein profilethreshold powertraining intensitytraining volume
spellingShingle Pieter deLange
Giuseppe Petito
Hannah L. Notbohm
Antonia Giacco
Giovanni Renzone
Elena Silvestri
Arianna Cuomo
Frank Suhr
Thorsten Schiffer
Jonas Zacher
Federica Cioffi
Rosalba Senese
Andrea Scaloni
Moritz Schumann
Wilhelm Bloch
Unaltered maximal power and submaximal performance correlates with an oxidative vastus lateralis proteome phenotype during tapering in male cyclists
Physiological Reports
aerobic capacity
fiber percentage ratio
protein profile
threshold power
training intensity
training volume
title Unaltered maximal power and submaximal performance correlates with an oxidative vastus lateralis proteome phenotype during tapering in male cyclists
title_full Unaltered maximal power and submaximal performance correlates with an oxidative vastus lateralis proteome phenotype during tapering in male cyclists
title_fullStr Unaltered maximal power and submaximal performance correlates with an oxidative vastus lateralis proteome phenotype during tapering in male cyclists
title_full_unstemmed Unaltered maximal power and submaximal performance correlates with an oxidative vastus lateralis proteome phenotype during tapering in male cyclists
title_short Unaltered maximal power and submaximal performance correlates with an oxidative vastus lateralis proteome phenotype during tapering in male cyclists
title_sort unaltered maximal power and submaximal performance correlates with an oxidative vastus lateralis proteome phenotype during tapering in male cyclists
topic aerobic capacity
fiber percentage ratio
protein profile
threshold power
training intensity
training volume
url https://doi.org/10.14814/phy2.70302
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