Acinetobacter indicus Coharboring Tet(X6) and blaNDM-1 Isolated From Slaughterhouse Waste
Objectives: Acinetobacter indicus is an important pathogen of nosocomial infection. The purpose of this study was to analyze the resistance and transmission of A. indicus strain AIBD14 isolated from slaughterhouse environment. Methods: A total of 96 environmental samples were collected from slaughte...
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Elsevier
2025-03-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716524004636 |
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| author | Xiaoqian Long Feng Lin Biao Tang Fengcheng Miao Zhiyu Li Yao Shen Hua Yang Jiangang Ma |
| author_facet | Xiaoqian Long Feng Lin Biao Tang Fengcheng Miao Zhiyu Li Yao Shen Hua Yang Jiangang Ma |
| author_sort | Xiaoqian Long |
| collection | DOAJ |
| description | Objectives: Acinetobacter indicus is an important pathogen of nosocomial infection. The purpose of this study was to analyze the resistance and transmission of A. indicus strain AIBD14 isolated from slaughterhouse environment. Methods: A total of 96 environmental samples were collected from slaughterhouse. The antimicrobial susceptibility test was carried out by microbroth dilution method and E-test. Whole genome sequencing and bioinformatics analysis of the AIBD14 were performed, then S1-PFGE and southern blot verified the location of blaNDM-1 and tet(X6). Results: The AIBD14 is resistant to meropenem but susceptibility to tigecycline, and coharboring blaNDM-1 and tet(X6). The blaNDM-1 is located on the pAIBD14-NDM-1 that cannot be transferred by conjugation. Specifically, blaNDM-1 is located on the transposon Tn125, and blaNDM-1 can be transferred to other species with the help of transposon. The genetic background of blaNDM-1 is ''ISAba125-blaNDM-1-bleMEL-dsbD-cutA-groES-groEL-insE-ISAba125''. pAIBD14-NDM-1 is classified into the GR31 plasmid based on the homology of the repB. Meanwhile, there are two XerC/D-like binding sites on the plasmid, which can mediate the transfer of resistance genes. The tet(X6) gene is located on the chromosome of AIBD14, its downstream is accompanied by the neglected macrolide resistance gene estT, and there is a single copy of the insertion element ISCR2 around tet(X6) as the genetic background ''ISAba4-IS3-hp-hp-tet(X6)-estT-guaA-ISCR2''. Conclusions: This is the first report of the coexistence of tet(X6) and blaNDM-1 in the A. indicus, and it has the risk of horizontal transfer across multiple species. So strict monitoring the multiple-resistant bacteria in the industrial chain is necessary based on the ''One Heath''. |
| format | Article |
| id | doaj-art-0c460fb1c09a44269d0d7dd2277bb56a |
| institution | OA Journals |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-0c460fb1c09a44269d0d7dd2277bb56a2025-08-20T01:57:35ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-03-01411710.1016/j.jgar.2024.12.004Acinetobacter indicus Coharboring Tet(X6) and blaNDM-1 Isolated From Slaughterhouse WasteXiaoqian Long0Feng Lin1Biao Tang2Fengcheng Miao3Zhiyu Li4Yao Shen5Hua Yang6Jiangang Ma7State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products & Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China; Xianghu Laboratory, Hangzhou, ChinaKey Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou, Zhejiang, ChinaKey Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, ChinaXianghu Laboratory, Hangzhou, ChinaXianghu Laboratory, Hangzhou, ChinaXianghu Laboratory, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products & Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China; Xianghu Laboratory, Hangzhou, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products & Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, China; Xianghu Laboratory, Hangzhou, China; Corresponding author. Jiangang Ma, Xianghu Laboratory, No.168 Gengwen Road, Hangzhou, 311200, China.Objectives: Acinetobacter indicus is an important pathogen of nosocomial infection. The purpose of this study was to analyze the resistance and transmission of A. indicus strain AIBD14 isolated from slaughterhouse environment. Methods: A total of 96 environmental samples were collected from slaughterhouse. The antimicrobial susceptibility test was carried out by microbroth dilution method and E-test. Whole genome sequencing and bioinformatics analysis of the AIBD14 were performed, then S1-PFGE and southern blot verified the location of blaNDM-1 and tet(X6). Results: The AIBD14 is resistant to meropenem but susceptibility to tigecycline, and coharboring blaNDM-1 and tet(X6). The blaNDM-1 is located on the pAIBD14-NDM-1 that cannot be transferred by conjugation. Specifically, blaNDM-1 is located on the transposon Tn125, and blaNDM-1 can be transferred to other species with the help of transposon. The genetic background of blaNDM-1 is ''ISAba125-blaNDM-1-bleMEL-dsbD-cutA-groES-groEL-insE-ISAba125''. pAIBD14-NDM-1 is classified into the GR31 plasmid based on the homology of the repB. Meanwhile, there are two XerC/D-like binding sites on the plasmid, which can mediate the transfer of resistance genes. The tet(X6) gene is located on the chromosome of AIBD14, its downstream is accompanied by the neglected macrolide resistance gene estT, and there is a single copy of the insertion element ISCR2 around tet(X6) as the genetic background ''ISAba4-IS3-hp-hp-tet(X6)-estT-guaA-ISCR2''. Conclusions: This is the first report of the coexistence of tet(X6) and blaNDM-1 in the A. indicus, and it has the risk of horizontal transfer across multiple species. So strict monitoring the multiple-resistant bacteria in the industrial chain is necessary based on the ''One Heath''.http://www.sciencedirect.com/science/article/pii/S2213716524004636Acinetobacter indicusTet(X6)BlaNDM-1Plasmid-mediated |
| spellingShingle | Xiaoqian Long Feng Lin Biao Tang Fengcheng Miao Zhiyu Li Yao Shen Hua Yang Jiangang Ma Acinetobacter indicus Coharboring Tet(X6) and blaNDM-1 Isolated From Slaughterhouse Waste Journal of Global Antimicrobial Resistance Acinetobacter indicus Tet(X6) BlaNDM-1 Plasmid-mediated |
| title | Acinetobacter indicus Coharboring Tet(X6) and blaNDM-1 Isolated From Slaughterhouse Waste |
| title_full | Acinetobacter indicus Coharboring Tet(X6) and blaNDM-1 Isolated From Slaughterhouse Waste |
| title_fullStr | Acinetobacter indicus Coharboring Tet(X6) and blaNDM-1 Isolated From Slaughterhouse Waste |
| title_full_unstemmed | Acinetobacter indicus Coharboring Tet(X6) and blaNDM-1 Isolated From Slaughterhouse Waste |
| title_short | Acinetobacter indicus Coharboring Tet(X6) and blaNDM-1 Isolated From Slaughterhouse Waste |
| title_sort | acinetobacter indicus coharboring tet x6 and blandm 1 isolated from slaughterhouse waste |
| topic | Acinetobacter indicus Tet(X6) BlaNDM-1 Plasmid-mediated |
| url | http://www.sciencedirect.com/science/article/pii/S2213716524004636 |
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