Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy
Abstract Conventional histology, as well as immunohistochemistry or immunofluorescence, enables the study of morphological and phenotypical changes during tissue inflammation with single-cell accuracy. However, although highly specific, such techniques require multiple time-consuming steps to apply...
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Nature Portfolio
2023-12-01
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| Series: | npj Imaging |
| Online Access: | https://doi.org/10.1038/s44303-023-00003-1 |
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| author | Vito Ko Marie C. Goess Lukas Scheel-Platz Tao Yuan Andriy Chmyrov Dominik Jüstel Jürgen Ruland Vasilis Ntziachristos Selina J. Keppler Miguel A. Pleitez |
| author_facet | Vito Ko Marie C. Goess Lukas Scheel-Platz Tao Yuan Andriy Chmyrov Dominik Jüstel Jürgen Ruland Vasilis Ntziachristos Selina J. Keppler Miguel A. Pleitez |
| author_sort | Vito Ko |
| collection | DOAJ |
| description | Abstract Conventional histology, as well as immunohistochemistry or immunofluorescence, enables the study of morphological and phenotypical changes during tissue inflammation with single-cell accuracy. However, although highly specific, such techniques require multiple time-consuming steps to apply exogenous labels, which might result in morphological deviations from native tissue structures. Unlike these techniques, mid-infrared (mid-IR) microspectroscopy is a label-free optical imaging method that retrieves endogenous biomolecular contrast without altering the native composition of the samples. Nevertheless, due to the strong optical absorption of water in biological tissues, conventional mid-IR microspectroscopy has been limited to dried thin (5–10 µm) tissue preparations and, thus, it also requires time-consuming steps—comparable to conventional imaging techniques. Here, as a step towards label-free analytical histology of unprocessed tissues, we applied mid-IR optoacoustic microscopy (MiROM) to retrieve intrinsic molecular contrast by vibrational excitation and, simultaneously, to overcome water-tissue opacity of conventional mid-IR imaging in thick (mm range) tissues. In this proof-of-concept study, we demonstrated application of MiROM for the fast, label-free, non-destructive assessment of the hallmarks of inflammation in excised white adipose tissue; i.e., formation of crown-like structures and changes in adipocyte morphology. |
| format | Article |
| id | doaj-art-0c3b814ec0b3401c85f397ee980a5db3 |
| institution | Kabale University |
| issn | 2948-197X |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Imaging |
| spelling | doaj-art-0c3b814ec0b3401c85f397ee980a5db32025-08-20T04:02:55ZengNature Portfolionpj Imaging2948-197X2023-12-011111210.1038/s44303-023-00003-1Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopyVito Ko0Marie C. Goess1Lukas Scheel-Platz2Tao Yuan3Andriy Chmyrov4Dominik Jüstel5Jürgen Ruland6Vasilis Ntziachristos7Selina J. Keppler8Miguel A. Pleitez9Chair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of MunichInstitute for Clinical Chemistry and Pathobiochemistry, Technical University of Munich, School of MedicineChair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of MunichChair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of MunichChair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of MunichChair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of MunichInstitute for Clinical Chemistry and Pathobiochemistry, Technical University of Munich, School of MedicineChair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of MunichInstitute for Clinical Chemistry and Pathobiochemistry, Technical University of Munich, School of MedicineChair of Biological Imaging at the Central Institute for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of MunichAbstract Conventional histology, as well as immunohistochemistry or immunofluorescence, enables the study of morphological and phenotypical changes during tissue inflammation with single-cell accuracy. However, although highly specific, such techniques require multiple time-consuming steps to apply exogenous labels, which might result in morphological deviations from native tissue structures. Unlike these techniques, mid-infrared (mid-IR) microspectroscopy is a label-free optical imaging method that retrieves endogenous biomolecular contrast without altering the native composition of the samples. Nevertheless, due to the strong optical absorption of water in biological tissues, conventional mid-IR microspectroscopy has been limited to dried thin (5–10 µm) tissue preparations and, thus, it also requires time-consuming steps—comparable to conventional imaging techniques. Here, as a step towards label-free analytical histology of unprocessed tissues, we applied mid-IR optoacoustic microscopy (MiROM) to retrieve intrinsic molecular contrast by vibrational excitation and, simultaneously, to overcome water-tissue opacity of conventional mid-IR imaging in thick (mm range) tissues. In this proof-of-concept study, we demonstrated application of MiROM for the fast, label-free, non-destructive assessment of the hallmarks of inflammation in excised white adipose tissue; i.e., formation of crown-like structures and changes in adipocyte morphology.https://doi.org/10.1038/s44303-023-00003-1 |
| spellingShingle | Vito Ko Marie C. Goess Lukas Scheel-Platz Tao Yuan Andriy Chmyrov Dominik Jüstel Jürgen Ruland Vasilis Ntziachristos Selina J. Keppler Miguel A. Pleitez Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy npj Imaging |
| title | Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy |
| title_full | Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy |
| title_fullStr | Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy |
| title_full_unstemmed | Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy |
| title_short | Fast histological assessment of adipose tissue inflammation by label-free mid-infrared optoacoustic microscopy |
| title_sort | fast histological assessment of adipose tissue inflammation by label free mid infrared optoacoustic microscopy |
| url | https://doi.org/10.1038/s44303-023-00003-1 |
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