Frequency of BK virus genotypes in patients with colorectal cancer
Background and Objectives: BK polyomavirus infection is prevalent and primarily asymptomatic, except for complications in kidney transplant recipients. Furthermore, its involvement in a tumorigenic family necessitates consideration in various malignancies such as urogenital tumors, prostate cancer,...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2025-02-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4947 |
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| author | Mahsa Javadi Gholamabbas Kaydani Roya Pirmoradi Abdolhassan Talaiezadeh Azadeh Haghi Navand Mohammad Karimi Baba Ahmadi Manoochehr Makvandi |
| author_facet | Mahsa Javadi Gholamabbas Kaydani Roya Pirmoradi Abdolhassan Talaiezadeh Azadeh Haghi Navand Mohammad Karimi Baba Ahmadi Manoochehr Makvandi |
| author_sort | Mahsa Javadi |
| collection | DOAJ |
| description |
Background and Objectives: BK polyomavirus infection is prevalent and primarily asymptomatic, except for complications in kidney transplant recipients. Furthermore, its involvement in a tumorigenic family necessitates consideration in various malignancies such as urogenital tumors, prostate cancer, colorectal cancer (CRC), and brain cancer.
Materials and Methods: This investigation encompassed 50 specimens of colorectal adenocarcinoma tumors, 50 adjacent tissues, and 40 urine samples, with patients having a mean age of 61 years ± 12.4 years. The detection of BK virus DNA VP1 gene and genotyping were carried out through nested-PCR and sequencing techniques.
Results: Through the utilization of nested-PCR, BK virus DNA was identified in 15/50 (30%) colorectal tumor samples and 3/50 (6%) adjacent tissues (p-value = 0.008). Additionally, 6/40 (15%) urine samples exhibited positive results for BK virus DNA. Notably, among these findings, 9/15 BK virus positive tumor tissues (60%) and 3/6 BK virus positive urine samples (50%) were confirmed to be positive for BK virus subtype 4 (p-value < 0.001), whereas 2 tumor samples and 3 urine samples were attributed to BK virus type 1b2.
Conclusion: It is imperative to enhance one's understanding of the etiological and risk factors pertaining to cancers. The present findings offer substantiation of a potential correlation between BK virus infection and colorectal cancer. BK virus genotype 4 was found to be dominant among the CRC patients in this study.
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| format | Article |
| id | doaj-art-0c3b171c29a2402994ed37d1e10e0af4 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-0c3b171c29a2402994ed37d1e10e0af42025-02-09T09:01:24ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472025-02-01171Frequency of BK virus genotypes in patients with colorectal cancerMahsa Javadi0Gholamabbas Kaydani1Roya Pirmoradi2Abdolhassan Talaiezadeh3Azadeh Haghi Navand4Mohammad Karimi Baba Ahmadi5Manoochehr Makvandi6Cancer Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Laboratory Sciences, School of Allied Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCancer Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCancer Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCancer Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; Department Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Medical Biotechnology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCancer Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Background and Objectives: BK polyomavirus infection is prevalent and primarily asymptomatic, except for complications in kidney transplant recipients. Furthermore, its involvement in a tumorigenic family necessitates consideration in various malignancies such as urogenital tumors, prostate cancer, colorectal cancer (CRC), and brain cancer. Materials and Methods: This investigation encompassed 50 specimens of colorectal adenocarcinoma tumors, 50 adjacent tissues, and 40 urine samples, with patients having a mean age of 61 years ± 12.4 years. The detection of BK virus DNA VP1 gene and genotyping were carried out through nested-PCR and sequencing techniques. Results: Through the utilization of nested-PCR, BK virus DNA was identified in 15/50 (30%) colorectal tumor samples and 3/50 (6%) adjacent tissues (p-value = 0.008). Additionally, 6/40 (15%) urine samples exhibited positive results for BK virus DNA. Notably, among these findings, 9/15 BK virus positive tumor tissues (60%) and 3/6 BK virus positive urine samples (50%) were confirmed to be positive for BK virus subtype 4 (p-value < 0.001), whereas 2 tumor samples and 3 urine samples were attributed to BK virus type 1b2. Conclusion: It is imperative to enhance one's understanding of the etiological and risk factors pertaining to cancers. The present findings offer substantiation of a potential correlation between BK virus infection and colorectal cancer. BK virus genotype 4 was found to be dominant among the CRC patients in this study. https://ijm.tums.ac.ir/index.php/ijm/article/view/4947BK polyomavirus;Colorectal cancer;Urine;Genotypes |
| spellingShingle | Mahsa Javadi Gholamabbas Kaydani Roya Pirmoradi Abdolhassan Talaiezadeh Azadeh Haghi Navand Mohammad Karimi Baba Ahmadi Manoochehr Makvandi Frequency of BK virus genotypes in patients with colorectal cancer Iranian Journal of Microbiology BK polyomavirus; Colorectal cancer; Urine; Genotypes |
| title | Frequency of BK virus genotypes in patients with colorectal cancer |
| title_full | Frequency of BK virus genotypes in patients with colorectal cancer |
| title_fullStr | Frequency of BK virus genotypes in patients with colorectal cancer |
| title_full_unstemmed | Frequency of BK virus genotypes in patients with colorectal cancer |
| title_short | Frequency of BK virus genotypes in patients with colorectal cancer |
| title_sort | frequency of bk virus genotypes in patients with colorectal cancer |
| topic | BK polyomavirus; Colorectal cancer; Urine; Genotypes |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4947 |
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