Screening and evaluation of specific blood MiRNAs as potential biomarkers in diagnostics of gastric Cancer
Abstract To provide a novel direction for the diagnosis of gastric cancer (GC). The differentially expressed blood microRNAs (miRNAs) in gastric carcinoma were screened through SangerBox using the datasets GSE113486, GSE112264, and GSE113740. The miRNA-target genes prediction, conduct Gene Ontology...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-06773-5 |
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| author | Xize Li Qingyu Wang Zhouhan Xu Xuesi Yang Ruiqi Zhao Haocheng Wang Xueling Li Jiping Zeng |
| author_facet | Xize Li Qingyu Wang Zhouhan Xu Xuesi Yang Ruiqi Zhao Haocheng Wang Xueling Li Jiping Zeng |
| author_sort | Xize Li |
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| description | Abstract To provide a novel direction for the diagnosis of gastric cancer (GC). The differentially expressed blood microRNAs (miRNAs) in gastric carcinoma were screened through SangerBox using the datasets GSE113486, GSE112264, and GSE113740. The miRNA-target genes prediction, conduct Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses were performed with the Database for Annotation, Visualization and Integrated Discovery (DAVID) 6.8. STRING analysis was further investigated with Cytoscape. The correlations among the expression levels of key miRNAs and prognosis/diagnostic value in GC patients were determined by survival prognosis and Receiver Operating Characteristic (ROC) curve analysis. Quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR) was employed to detect the different expression levels of key miRNAs in human blood samples. In the process, the influence of Helicobacter pylori (H. pylori) infection on expression levels of miRNAs was analyzed with Gene Expression Omnibus 2R (GEO2R) in dataset GSE108307. To evaluate these findings, the expression level of Cytotoxin-associated gene A (CagA), along with clinical markers used in the help of GC pathologic diagnosis, were measured and compared with the key miRNAs obtained in this study. The bioinformatics analysis identified five crucial blood miRNAs, including hsa-miR-124-3p, hsa-miR-125a-3p, hsa-miR-29b-3p, hsa-miR-4276, and hsa-miR-575. The detection in human blood samples combined with cross-analysis involving H. pylori infection, the expression levels of CagA and clinical markers underscored the significance and effectiveness of these specific miRNAs in early diagnosis and monitoring of gastric carcinoma. This study identified five potential blood miRNA biomarkers for GC through bioinformatics analysis coupled with detection in human blood samples, thus providing new possibilities for important biomarkers related to diagnosis and prognosis of GC. |
| format | Article |
| id | doaj-art-0c1c693381e44ab4bb5ad92d7d09e252 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-0c1c693381e44ab4bb5ad92d7d09e2522025-08-20T03:03:32ZengNature PortfolioScientific Reports2045-23222025-07-0115111310.1038/s41598-025-06773-5Screening and evaluation of specific blood MiRNAs as potential biomarkers in diagnostics of gastric CancerXize Li0Qingyu Wang1Zhouhan Xu2Xuesi Yang3Ruiqi Zhao4Haocheng Wang5Xueling Li6Jiping Zeng7Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong UniversityDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong UniversityClinical Five-Year Program, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong UniversityClinical Five-Year Program, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong UniversityClinical Five-Year Program, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong UniversityClinical Eight-Year Program, Shanghai Jiao Tong University School of MedicineDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong UniversityDepartment of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong UniversityAbstract To provide a novel direction for the diagnosis of gastric cancer (GC). The differentially expressed blood microRNAs (miRNAs) in gastric carcinoma were screened through SangerBox using the datasets GSE113486, GSE112264, and GSE113740. The miRNA-target genes prediction, conduct Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses were performed with the Database for Annotation, Visualization and Integrated Discovery (DAVID) 6.8. STRING analysis was further investigated with Cytoscape. The correlations among the expression levels of key miRNAs and prognosis/diagnostic value in GC patients were determined by survival prognosis and Receiver Operating Characteristic (ROC) curve analysis. Quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR) was employed to detect the different expression levels of key miRNAs in human blood samples. In the process, the influence of Helicobacter pylori (H. pylori) infection on expression levels of miRNAs was analyzed with Gene Expression Omnibus 2R (GEO2R) in dataset GSE108307. To evaluate these findings, the expression level of Cytotoxin-associated gene A (CagA), along with clinical markers used in the help of GC pathologic diagnosis, were measured and compared with the key miRNAs obtained in this study. The bioinformatics analysis identified five crucial blood miRNAs, including hsa-miR-124-3p, hsa-miR-125a-3p, hsa-miR-29b-3p, hsa-miR-4276, and hsa-miR-575. The detection in human blood samples combined with cross-analysis involving H. pylori infection, the expression levels of CagA and clinical markers underscored the significance and effectiveness of these specific miRNAs in early diagnosis and monitoring of gastric carcinoma. This study identified five potential blood miRNA biomarkers for GC through bioinformatics analysis coupled with detection in human blood samples, thus providing new possibilities for important biomarkers related to diagnosis and prognosis of GC.https://doi.org/10.1038/s41598-025-06773-5Gastric cancerBlood-based MiRNAsDifferentially expressed genesBiomarkersBioinformatics |
| spellingShingle | Xize Li Qingyu Wang Zhouhan Xu Xuesi Yang Ruiqi Zhao Haocheng Wang Xueling Li Jiping Zeng Screening and evaluation of specific blood MiRNAs as potential biomarkers in diagnostics of gastric Cancer Scientific Reports Gastric cancer Blood-based MiRNAs Differentially expressed genes Biomarkers Bioinformatics |
| title | Screening and evaluation of specific blood MiRNAs as potential biomarkers in diagnostics of gastric Cancer |
| title_full | Screening and evaluation of specific blood MiRNAs as potential biomarkers in diagnostics of gastric Cancer |
| title_fullStr | Screening and evaluation of specific blood MiRNAs as potential biomarkers in diagnostics of gastric Cancer |
| title_full_unstemmed | Screening and evaluation of specific blood MiRNAs as potential biomarkers in diagnostics of gastric Cancer |
| title_short | Screening and evaluation of specific blood MiRNAs as potential biomarkers in diagnostics of gastric Cancer |
| title_sort | screening and evaluation of specific blood mirnas as potential biomarkers in diagnostics of gastric cancer |
| topic | Gastric cancer Blood-based MiRNAs Differentially expressed genes Biomarkers Bioinformatics |
| url | https://doi.org/10.1038/s41598-025-06773-5 |
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