Emerging multi-drug resistant and extended-spectrum β-lactamase (ESBL)-positive enterotoxigenic E. coli (ETEC) clones circulating in aquatic environments and in patients

Diarrheal disease pathogens often spread through water-borne routes. Enterotoxigenic Escherichia coli (ETEC) is a major bacterial agent causing diarrheal disease in children, adults, and travelers in endemic areas. In addition, ETEC is responsible for outbreaks of water and food-borne gasteroenterit...

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Main Authors: Enrique Joffré, Alberto J. Martín-Rodríguez, Annie Justh de Neczpal, Astrid von Mentzer, Åsa Sjöling
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:One Health
Online Access:http://www.sciencedirect.com/science/article/pii/S2352771425000047
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author Enrique Joffré
Alberto J. Martín-Rodríguez
Annie Justh de Neczpal
Astrid von Mentzer
Åsa Sjöling
author_facet Enrique Joffré
Alberto J. Martín-Rodríguez
Annie Justh de Neczpal
Astrid von Mentzer
Åsa Sjöling
author_sort Enrique Joffré
collection DOAJ
description Diarrheal disease pathogens often spread through water-borne routes. Enterotoxigenic Escherichia coli (ETEC) is a major bacterial agent causing diarrheal disease in children, adults, and travelers in endemic areas. In addition, ETEC is responsible for outbreaks of water and food-borne gasteroenteritis globally, ETEC isolates also show robust survival capacity in various environmental settings, including aquatic environments.During the last decade, studies of ETEC isolates have indicated a rapid increase in multi-drug resistant and extended-spectrum β-lactamase (ESBL)-positive human-specific ETEC strains. These have been found in both environmental water sources and human patients, warranting the urgent need for focused monitoring of antibiotic resistance development in ETEC.Whole genome sequencing (WGS) of isolates from environmental, animal, and human sources enables in silico surveillance of emerging pathogenic and multi-drug resistant strains. This method allows for re-analysis of genomic data, aiding in identification of new variants of pathogenic clones.By integrating data from diverse sources inclusing sequenced isolates, we found that certain ETEC clonal lineages e.g., those expressing certain toxin-colonization factor profiles including STp/CS6, LT STh/CS2 + CS3, and LT STh/CFA/I are more at risk to develop multi-drug resistance than other ETEC lineages. Comparizon of multi-locus sequence types from papers with WGS data indicated ST182, ST4, ST2332 and new ST types to be emerging multi-drug resistant ETEC.We conclude that further studies on sequenced ETEC/E. coli genomes are needed to enhance our understanding of the dynamics of ETEC evolution, and the relation of virulence and resistance profiles in both environmental and clinical isolates.
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spelling doaj-art-0c19bff03c1e4ef698e7a75be52ec9cb2025-08-20T03:47:20ZengElsevierOne Health2352-77142025-06-012010096810.1016/j.onehlt.2025.100968Emerging multi-drug resistant and extended-spectrum β-lactamase (ESBL)-positive enterotoxigenic E. coli (ETEC) clones circulating in aquatic environments and in patientsEnrique Joffré0Alberto J. Martín-Rodríguez1Annie Justh de Neczpal2Astrid von Mentzer3Åsa Sjöling4Department of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden; Department of Microbiology and Tumor Biology, Karolinska Institutet, Stockholm, SwedenDepartment of Microbiology and Tumor Biology, Karolinska Institutet, Stockholm, Sweden; Department of Clinical Sciences, Universtiy of Las Palmas de Gran Canaria, Las Palmas de Gran Canaria, SpainDepartment of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, SwedenDepartment of Microbiology and Immunology, University of Gothenburg, Gothenburg, SwedenDepartment of Chemistry and Molecular Biology, University of Gothenburg, Gothenburg, Sweden; Department of Microbiology and Tumor Biology, Karolinska Institutet, Stockholm, Sweden; Corresponding author at: Department of Chemistry and Molecular Biology, University of Gothenburg, Box 462 40530, Gothenburg, Sweden.Diarrheal disease pathogens often spread through water-borne routes. Enterotoxigenic Escherichia coli (ETEC) is a major bacterial agent causing diarrheal disease in children, adults, and travelers in endemic areas. In addition, ETEC is responsible for outbreaks of water and food-borne gasteroenteritis globally, ETEC isolates also show robust survival capacity in various environmental settings, including aquatic environments.During the last decade, studies of ETEC isolates have indicated a rapid increase in multi-drug resistant and extended-spectrum β-lactamase (ESBL)-positive human-specific ETEC strains. These have been found in both environmental water sources and human patients, warranting the urgent need for focused monitoring of antibiotic resistance development in ETEC.Whole genome sequencing (WGS) of isolates from environmental, animal, and human sources enables in silico surveillance of emerging pathogenic and multi-drug resistant strains. This method allows for re-analysis of genomic data, aiding in identification of new variants of pathogenic clones.By integrating data from diverse sources inclusing sequenced isolates, we found that certain ETEC clonal lineages e.g., those expressing certain toxin-colonization factor profiles including STp/CS6, LT STh/CS2 + CS3, and LT STh/CFA/I are more at risk to develop multi-drug resistance than other ETEC lineages. Comparizon of multi-locus sequence types from papers with WGS data indicated ST182, ST4, ST2332 and new ST types to be emerging multi-drug resistant ETEC.We conclude that further studies on sequenced ETEC/E. coli genomes are needed to enhance our understanding of the dynamics of ETEC evolution, and the relation of virulence and resistance profiles in both environmental and clinical isolates.http://www.sciencedirect.com/science/article/pii/S2352771425000047
spellingShingle Enrique Joffré
Alberto J. Martín-Rodríguez
Annie Justh de Neczpal
Astrid von Mentzer
Åsa Sjöling
Emerging multi-drug resistant and extended-spectrum β-lactamase (ESBL)-positive enterotoxigenic E. coli (ETEC) clones circulating in aquatic environments and in patients
One Health
title Emerging multi-drug resistant and extended-spectrum β-lactamase (ESBL)-positive enterotoxigenic E. coli (ETEC) clones circulating in aquatic environments and in patients
title_full Emerging multi-drug resistant and extended-spectrum β-lactamase (ESBL)-positive enterotoxigenic E. coli (ETEC) clones circulating in aquatic environments and in patients
title_fullStr Emerging multi-drug resistant and extended-spectrum β-lactamase (ESBL)-positive enterotoxigenic E. coli (ETEC) clones circulating in aquatic environments and in patients
title_full_unstemmed Emerging multi-drug resistant and extended-spectrum β-lactamase (ESBL)-positive enterotoxigenic E. coli (ETEC) clones circulating in aquatic environments and in patients
title_short Emerging multi-drug resistant and extended-spectrum β-lactamase (ESBL)-positive enterotoxigenic E. coli (ETEC) clones circulating in aquatic environments and in patients
title_sort emerging multi drug resistant and extended spectrum β lactamase esbl positive enterotoxigenic e coli etec clones circulating in aquatic environments and in patients
url http://www.sciencedirect.com/science/article/pii/S2352771425000047
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