Detection of Babesia spp by using of Acridine Orange and Giemsa Stain with Study of its Effect on Blood Picture in Local Breed Cows
This study was conducted to diagnose of Babesia spp by using fluorescent microscope and light microscope, the study also included evaluating of Hemogram (RBC, HB, PCV, MCV, MCHC, Total Leukocyte Count, Differential Leukocyte Count) in cows infected with Babesia spp which cause piroplasmosis. The stu...
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| Format: | Article |
| Language: | English |
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College of Veterinary Medicine, University of Fallujah
2019-12-01
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| Series: | مجلة الانبار للعلوم البيطرية |
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| Online Access: | https://www.anbarjvs.edu.iq/wp-content/uploads/2020/04/6.pdf |
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| author | Dhiyaa A. Moosa, Ahmed N. Flayyih Haneen B. Mohammed Nouri Omar M. Meshal |
| author_facet | Dhiyaa A. Moosa, Ahmed N. Flayyih Haneen B. Mohammed Nouri Omar M. Meshal |
| author_sort | Dhiyaa A. Moosa, Ahmed N. Flayyih |
| collection | DOAJ |
| description | This study was conducted to diagnose of Babesia spp by using fluorescent microscope and light microscope, the study also included evaluating of Hemogram (RBC, HB, PCV, MCV, MCHC, Total Leukocyte Count, Differential Leukocyte Count) in cows infected with Babesia spp which cause piroplasmosis. The study included examination of 60 local breed Cows from many districts of Mosul city from the beginning of February 2018 until the end of July 2018, 17 were clinically infected with piroplasmosis and the infection was confirmed by using Giemsa and Acridine orange staining blood smears, and 10 cows were clinically intact and served as control. the results indicated that the percentage of the infection with Babesia spp was 28.33%, and the percentage of parasitemia ranged between (5-23%) with mean of (12.57%). The Results showed a statistical significant decrease (P<0.05) in RBC, Hb, and PCV values of diseased animals, Normocytic Normochromic type of anemia was found, the results also indicated a significant decrease in total Leukocyte count and neutrophil, with significant increase in lymphocytes number. the results also indicated non-significant changes in Eosinophil, Basophil and Monocyte numbers |
| format | Article |
| id | doaj-art-0c199b90f7d84b689eadda01b7e9e5c2 |
| institution | Kabale University |
| issn | 1999-6527 2707-0603 |
| language | English |
| publishDate | 2019-12-01 |
| publisher | College of Veterinary Medicine, University of Fallujah |
| record_format | Article |
| series | مجلة الانبار للعلوم البيطرية |
| spelling | doaj-art-0c199b90f7d84b689eadda01b7e9e5c22025-08-20T03:58:07ZengCollege of Veterinary Medicine, University of Fallujahمجلة الانبار للعلوم البيطرية1999-65272707-06032019-12-0112210.37940/AJVS.2019.12.2.6Detection of Babesia spp by using of Acridine Orange and Giemsa Stain with Study of its Effect on Blood Picture in Local Breed CowsDhiyaa A. Moosa, Ahmed N. FlayyihHaneen B. Mohammed NouriOmar M. Meshal This study was conducted to diagnose of Babesia spp by using fluorescent microscope and light microscope, the study also included evaluating of Hemogram (RBC, HB, PCV, MCV, MCHC, Total Leukocyte Count, Differential Leukocyte Count) in cows infected with Babesia spp which cause piroplasmosis. The study included examination of 60 local breed Cows from many districts of Mosul city from the beginning of February 2018 until the end of July 2018, 17 were clinically infected with piroplasmosis and the infection was confirmed by using Giemsa and Acridine orange staining blood smears, and 10 cows were clinically intact and served as control. the results indicated that the percentage of the infection with Babesia spp was 28.33%, and the percentage of parasitemia ranged between (5-23%) with mean of (12.57%). The Results showed a statistical significant decrease (P<0.05) in RBC, Hb, and PCV values of diseased animals, Normocytic Normochromic type of anemia was found, the results also indicated a significant decrease in total Leukocyte count and neutrophil, with significant increase in lymphocytes number. the results also indicated non-significant changes in Eosinophil, Basophil and Monocyte numbershttps://www.anbarjvs.edu.iq/wp-content/uploads/2020/04/6.pdfbabesiaheamatologycow |
| spellingShingle | Dhiyaa A. Moosa, Ahmed N. Flayyih Haneen B. Mohammed Nouri Omar M. Meshal Detection of Babesia spp by using of Acridine Orange and Giemsa Stain with Study of its Effect on Blood Picture in Local Breed Cows مجلة الانبار للعلوم البيطرية babesia heamatology cow |
| title | Detection of Babesia spp by using of Acridine Orange and Giemsa Stain with Study of its Effect on Blood Picture in Local Breed Cows |
| title_full | Detection of Babesia spp by using of Acridine Orange and Giemsa Stain with Study of its Effect on Blood Picture in Local Breed Cows |
| title_fullStr | Detection of Babesia spp by using of Acridine Orange and Giemsa Stain with Study of its Effect on Blood Picture in Local Breed Cows |
| title_full_unstemmed | Detection of Babesia spp by using of Acridine Orange and Giemsa Stain with Study of its Effect on Blood Picture in Local Breed Cows |
| title_short | Detection of Babesia spp by using of Acridine Orange and Giemsa Stain with Study of its Effect on Blood Picture in Local Breed Cows |
| title_sort | detection of babesia spp by using of acridine orange and giemsa stain with study of its effect on blood picture in local breed cows |
| topic | babesia heamatology cow |
| url | https://www.anbarjvs.edu.iq/wp-content/uploads/2020/04/6.pdf |
| work_keys_str_mv | AT dhiyaaamoosaahmednflayyih detectionofbabesiasppbyusingofacridineorangeandgiemsastainwithstudyofitseffectonbloodpictureinlocalbreedcows AT haneenbmohammednouri detectionofbabesiasppbyusingofacridineorangeandgiemsastainwithstudyofitseffectonbloodpictureinlocalbreedcows AT omarmmeshal detectionofbabesiasppbyusingofacridineorangeandgiemsastainwithstudyofitseffectonbloodpictureinlocalbreedcows |