Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1
Abstract Background This research aims to explore the anti-obesity potential of Wu-Mei-Wan (WMW), particularly its effects on adipose tissue regulation in obese mice induced by a high-fat diet (HFD). The study focuses on understanding the role of heat shock factor 1 (HSF1) in mediating these effects...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | Chinese Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13020-024-01053-2 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559116065013760 |
|---|---|
| author | Shen Chen Kexin Nie Hongzhan Wang Yang Gao Xinyue Jiang Hao Su Zhi Wang Yueheng Tang Fuer Lu Hui Dong Jingbin Li |
| author_facet | Shen Chen Kexin Nie Hongzhan Wang Yang Gao Xinyue Jiang Hao Su Zhi Wang Yueheng Tang Fuer Lu Hui Dong Jingbin Li |
| author_sort | Shen Chen |
| collection | DOAJ |
| description | Abstract Background This research aims to explore the anti-obesity potential of Wu-Mei-Wan (WMW), particularly its effects on adipose tissue regulation in obese mice induced by a high-fat diet (HFD). The study focuses on understanding the role of heat shock factor 1 (HSF1) in mediating these effects. Methods HFD-induced obese mice were treated with WMW. Body weight, food intake, and histopathological analysis of adipose tissue were conducted. Brown adipose tissue (BAT) activity was evaluated using Positron Emission Tomography, and ultrastructural changes were examined via transmission electron microscopy. Proteomic analysis identified targets of WMW in obesity treatment. HSF1 expression was inhibited to confirm its role. Molecular docking studied interactions between WMW and HSF1. Short-chain fatty acids (SCFAs) in the intestines were measured to determine if WMW’s effects on HSF1 are mediated through SCFAs. Protein expression was assessed using western blot, immunohistochemistry, immunofluorescence and RT-qPCR were employed to detect the mRNA levels. Statistical analyses included t-tests, ANOVA, and non-parametric tests like the Mann–Whitney U test or Kruskal–Wallis test. Results WMW significantly mitigates the adverse effects of a HFD on body weight and glucose metabolism in obese mice. Both low-dose WMW and high-dose WMW treatments led to reduced weight gain and improved glucose tolerance, with low-dose WMW showing more pronounced effects. WMW also reversed structural damage in BAT, enhancing mitochondrial integrity and thermogenic function, particularly at the low dose. Additionally, WMW treatment promoted the browning of WAT, evidenced by increased expression of key thermogenic proteins such as UCP1 and PGC-1α. The increase in HSF1 expression in both BAT and WAT, observed with WMW treatment, was crucial for these beneficial effects, as inhibition of HSF1 negated the positive outcomes. Furthermore, WMW treatment led to elevated levels of short-chain fatty acids SCFAs in the intestines, which are associated with increased HSF1 expression. Conclusions WMW represents a potent therapeutic strategy for obesity, promoting metabolic health and beneficial modulation of adipose tissue through an HSF1-dependent pathway. |
| format | Article |
| id | doaj-art-0bfae0e5f2bb44e68d0f7261de230bb9 |
| institution | Kabale University |
| issn | 1749-8546 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj-art-0bfae0e5f2bb44e68d0f7261de230bb92025-01-05T12:46:05ZengBMCChinese Medicine1749-85462025-01-0120112210.1186/s13020-024-01053-2Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1Shen Chen0Kexin Nie1Hongzhan Wang2Yang Gao3Xinyue Jiang4Hao Su5Zhi Wang6Yueheng Tang7Fuer Lu8Hui Dong9Jingbin Li10Institute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyInstitute of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyDepartment of Integrated Traditional Chinese and Western Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and TechnologyAbstract Background This research aims to explore the anti-obesity potential of Wu-Mei-Wan (WMW), particularly its effects on adipose tissue regulation in obese mice induced by a high-fat diet (HFD). The study focuses on understanding the role of heat shock factor 1 (HSF1) in mediating these effects. Methods HFD-induced obese mice were treated with WMW. Body weight, food intake, and histopathological analysis of adipose tissue were conducted. Brown adipose tissue (BAT) activity was evaluated using Positron Emission Tomography, and ultrastructural changes were examined via transmission electron microscopy. Proteomic analysis identified targets of WMW in obesity treatment. HSF1 expression was inhibited to confirm its role. Molecular docking studied interactions between WMW and HSF1. Short-chain fatty acids (SCFAs) in the intestines were measured to determine if WMW’s effects on HSF1 are mediated through SCFAs. Protein expression was assessed using western blot, immunohistochemistry, immunofluorescence and RT-qPCR were employed to detect the mRNA levels. Statistical analyses included t-tests, ANOVA, and non-parametric tests like the Mann–Whitney U test or Kruskal–Wallis test. Results WMW significantly mitigates the adverse effects of a HFD on body weight and glucose metabolism in obese mice. Both low-dose WMW and high-dose WMW treatments led to reduced weight gain and improved glucose tolerance, with low-dose WMW showing more pronounced effects. WMW also reversed structural damage in BAT, enhancing mitochondrial integrity and thermogenic function, particularly at the low dose. Additionally, WMW treatment promoted the browning of WAT, evidenced by increased expression of key thermogenic proteins such as UCP1 and PGC-1α. The increase in HSF1 expression in both BAT and WAT, observed with WMW treatment, was crucial for these beneficial effects, as inhibition of HSF1 negated the positive outcomes. Furthermore, WMW treatment led to elevated levels of short-chain fatty acids SCFAs in the intestines, which are associated with increased HSF1 expression. Conclusions WMW represents a potent therapeutic strategy for obesity, promoting metabolic health and beneficial modulation of adipose tissue through an HSF1-dependent pathway.https://doi.org/10.1186/s13020-024-01053-2Wu-Mei-WanObesityHeat shock factor 1White adipose tissueBrown adipose tissue |
| spellingShingle | Shen Chen Kexin Nie Hongzhan Wang Yang Gao Xinyue Jiang Hao Su Zhi Wang Yueheng Tang Fuer Lu Hui Dong Jingbin Li Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1 Chinese Medicine Wu-Mei-Wan Obesity Heat shock factor 1 White adipose tissue Brown adipose tissue |
| title | Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1 |
| title_full | Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1 |
| title_fullStr | Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1 |
| title_full_unstemmed | Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1 |
| title_short | Wu-Mei-Wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of HSF1 |
| title_sort | wu mei wan enhances brown adipose tissue function and white adipose browning in obese mice via upregulation of hsf1 |
| topic | Wu-Mei-Wan Obesity Heat shock factor 1 White adipose tissue Brown adipose tissue |
| url | https://doi.org/10.1186/s13020-024-01053-2 |
| work_keys_str_mv | AT shenchen wumeiwanenhancesbrownadiposetissuefunctionandwhiteadiposebrowninginobesemiceviaupregulationofhsf1 AT kexinnie wumeiwanenhancesbrownadiposetissuefunctionandwhiteadiposebrowninginobesemiceviaupregulationofhsf1 AT hongzhanwang wumeiwanenhancesbrownadiposetissuefunctionandwhiteadiposebrowninginobesemiceviaupregulationofhsf1 AT yanggao wumeiwanenhancesbrownadiposetissuefunctionandwhiteadiposebrowninginobesemiceviaupregulationofhsf1 AT xinyuejiang wumeiwanenhancesbrownadiposetissuefunctionandwhiteadiposebrowninginobesemiceviaupregulationofhsf1 AT haosu wumeiwanenhancesbrownadiposetissuefunctionandwhiteadiposebrowninginobesemiceviaupregulationofhsf1 AT zhiwang wumeiwanenhancesbrownadiposetissuefunctionandwhiteadiposebrowninginobesemiceviaupregulationofhsf1 AT yuehengtang wumeiwanenhancesbrownadiposetissuefunctionandwhiteadiposebrowninginobesemiceviaupregulationofhsf1 AT fuerlu wumeiwanenhancesbrownadiposetissuefunctionandwhiteadiposebrowninginobesemiceviaupregulationofhsf1 AT huidong wumeiwanenhancesbrownadiposetissuefunctionandwhiteadiposebrowninginobesemiceviaupregulationofhsf1 AT jingbinli wumeiwanenhancesbrownadiposetissuefunctionandwhiteadiposebrowninginobesemiceviaupregulationofhsf1 |