Generation of a new Paneth cell–specific Cre-recombinase transgenic mouse line
Paneth cells are located in the crypts of Lieberkühn in mammalian small intestines and are producing antimicrobial peptides to keep the microbiome under control. The genetic manipulation of Paneth cells and their tracking and depletion depend on a solid Paneth cell–specific Cre-transgenic line. Here...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-06-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1576995/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850169941430370304 |
|---|---|
| author | Natalia Garcia-Gonzalez Natalia Garcia-Gonzalez Charlotte Wallaeys Charlotte Wallaeys Wendy Toussaint Wendy Toussaint Tino Hochepied Tino Hochepied Sylviane Dewaele Sylviane Dewaele Somara De Beul Somara De Beul Robin Van Loke Robin Van Loke Jolien Vandewalle Jolien Vandewalle Steven Timmermans Steven Timmermans Claude Libert Claude Libert |
| author_facet | Natalia Garcia-Gonzalez Natalia Garcia-Gonzalez Charlotte Wallaeys Charlotte Wallaeys Wendy Toussaint Wendy Toussaint Tino Hochepied Tino Hochepied Sylviane Dewaele Sylviane Dewaele Somara De Beul Somara De Beul Robin Van Loke Robin Van Loke Jolien Vandewalle Jolien Vandewalle Steven Timmermans Steven Timmermans Claude Libert Claude Libert |
| author_sort | Natalia Garcia-Gonzalez |
| collection | DOAJ |
| description | Paneth cells are located in the crypts of Lieberkühn in mammalian small intestines and are producing antimicrobial peptides to keep the microbiome under control. The genetic manipulation of Paneth cells and their tracking and depletion depend on a solid Paneth cell–specific Cre-transgenic line. Here, we describe bulk RNA sequencing (RNA-seq)–based expression data from pure, sorted Paneth cells of C57BL/6J mice and identify several strongly expressed Paneth cell–specific genes, the expression of which is stable under pathophysiological conditions, as well as in the duodenum, jejunum, and ileum. We selected the Defa24 gene regulatory sequences and generated a new Defa24iCre transgenic line using BAC technology, Tg(Defa24-icre)Cli. The resulting transgenic line provides robust expression and allows for the complete depletion of Paneth cells by cell ablation, yielding mice without any detectable lysozyme biological activity in the small intestines. |
| format | Article |
| id | doaj-art-0be5dcfb99e643e6b6f1487b1951d957 |
| institution | OA Journals |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-0be5dcfb99e643e6b6f1487b1951d9572025-08-20T02:20:37ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-06-011610.3389/fimmu.2025.15769951576995Generation of a new Paneth cell–specific Cre-recombinase transgenic mouse lineNatalia Garcia-Gonzalez0Natalia Garcia-Gonzalez1Charlotte Wallaeys2Charlotte Wallaeys3Wendy Toussaint4Wendy Toussaint5Tino Hochepied6Tino Hochepied7Sylviane Dewaele8Sylviane Dewaele9Somara De Beul10Somara De Beul11Robin Van Loke12Robin Van Loke13Jolien Vandewalle14Jolien Vandewalle15Steven Timmermans16Steven Timmermans17Claude Libert18Claude Libert19Flanders Institute for Biotechnology (VIB) Center for Inflammation Research, Ghent, BelgiumDepartment of Biomedical Molecular Biology, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology (VIB) Center for Inflammation Research, Ghent, BelgiumDepartment of Biomedical Molecular Biology, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology (VIB) Center for Inflammation Research, Ghent, BelgiumDepartment of Biomedical Molecular Biology, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology (VIB) Center for Inflammation Research, Ghent, BelgiumDepartment of Biomedical Molecular Biology, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology (VIB) Center for Inflammation Research, Ghent, BelgiumDepartment of Biomedical Molecular Biology, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology (VIB) Center for Inflammation Research, Ghent, BelgiumDepartment of Biomedical Molecular Biology, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology (VIB) Center for Inflammation Research, Ghent, BelgiumDepartment of Biomedical Molecular Biology, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology (VIB) Center for Inflammation Research, Ghent, BelgiumDepartment of Biomedical Molecular Biology, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology (VIB) Center for Inflammation Research, Ghent, BelgiumDepartment of Biomedical Molecular Biology, Ghent University, Ghent, BelgiumFlanders Institute for Biotechnology (VIB) Center for Inflammation Research, Ghent, BelgiumDepartment of Biomedical Molecular Biology, Ghent University, Ghent, BelgiumPaneth cells are located in the crypts of Lieberkühn in mammalian small intestines and are producing antimicrobial peptides to keep the microbiome under control. The genetic manipulation of Paneth cells and their tracking and depletion depend on a solid Paneth cell–specific Cre-transgenic line. Here, we describe bulk RNA sequencing (RNA-seq)–based expression data from pure, sorted Paneth cells of C57BL/6J mice and identify several strongly expressed Paneth cell–specific genes, the expression of which is stable under pathophysiological conditions, as well as in the duodenum, jejunum, and ileum. We selected the Defa24 gene regulatory sequences and generated a new Defa24iCre transgenic line using BAC technology, Tg(Defa24-icre)Cli. The resulting transgenic line provides robust expression and allows for the complete depletion of Paneth cells by cell ablation, yielding mice without any detectable lysozyme biological activity in the small intestines.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1576995/fullPaneth cellsDefa24iCredefensinsCre-recombinase transgenic mouse modelCre/loxP |
| spellingShingle | Natalia Garcia-Gonzalez Natalia Garcia-Gonzalez Charlotte Wallaeys Charlotte Wallaeys Wendy Toussaint Wendy Toussaint Tino Hochepied Tino Hochepied Sylviane Dewaele Sylviane Dewaele Somara De Beul Somara De Beul Robin Van Loke Robin Van Loke Jolien Vandewalle Jolien Vandewalle Steven Timmermans Steven Timmermans Claude Libert Claude Libert Generation of a new Paneth cell–specific Cre-recombinase transgenic mouse line Frontiers in Immunology Paneth cells Defa24iCre defensins Cre-recombinase transgenic mouse model Cre/loxP |
| title | Generation of a new Paneth cell–specific Cre-recombinase transgenic mouse line |
| title_full | Generation of a new Paneth cell–specific Cre-recombinase transgenic mouse line |
| title_fullStr | Generation of a new Paneth cell–specific Cre-recombinase transgenic mouse line |
| title_full_unstemmed | Generation of a new Paneth cell–specific Cre-recombinase transgenic mouse line |
| title_short | Generation of a new Paneth cell–specific Cre-recombinase transgenic mouse line |
| title_sort | generation of a new paneth cell specific cre recombinase transgenic mouse line |
| topic | Paneth cells Defa24iCre defensins Cre-recombinase transgenic mouse model Cre/loxP |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1576995/full |
| work_keys_str_mv | AT nataliagarciagonzalez generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT nataliagarciagonzalez generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT charlottewallaeys generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT charlottewallaeys generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT wendytoussaint generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT wendytoussaint generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT tinohochepied generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT tinohochepied generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT sylvianedewaele generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT sylvianedewaele generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT somaradebeul generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT somaradebeul generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT robinvanloke generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT robinvanloke generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT jolienvandewalle generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT jolienvandewalle generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT steventimmermans generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT steventimmermans generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT claudelibert generationofanewpanethcellspecificcrerecombinasetransgenicmouseline AT claudelibert generationofanewpanethcellspecificcrerecombinasetransgenicmouseline |